Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 84 for “"Midgut"”.
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A Metagenomic Study of The Tick Midgut
<strong>A Metagenomic Study of the Tick Midgut<br></strong> Daniel Yuan, B.S.<br> Supervisory Professor : Steven J. Norris, Ph.D.<br> Southern tick–associated rash illness (STARI) or Master’s disease is a Lyme-like illness that occurs following bites by <em>Amblyomma americanum</em>, the lone-star …
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The role of midgut serine proteases in Aedes aegypti vector competence
… tract may exploit serine proteases in the midgut to enhance vector infection. Recent genetic and biochemical experiments have demonstrated that dengue virus type 2 (DENV-2) may require proteolytic processing by midgut trypsins to efficiently infect Aedes aegypti. These results suggest …
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Linking mosquito midgut and virus population biology at the molecular and cellular level
… arbovirus emergence and evolution. The mosquito midgut functions as a key interface between virus and vector. However, studies of midgut physiology and associated virus infection dynamics are scarce, and in Culex tarsalis - the primary vector of West Nile virus (WNV) in the contiguous United …
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Sodium and glucose transport across the in vitro perfused midgut of the blue crab, Callinectes sapidus Rathbun
… (1) to investigate sodium transport across the midgut as well as the hindgut of Callinectes sapidus and the functioning of these two regions of the gut in osmotic and ionic regulation of the animal, and (2) to elucidate the mechanism of glucose absorption across the midgut of C. sapidus and to …
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Characterization of the molecular and biological determinants of viral dissemination from the mosquito midgut: Yellow feber virus in Aedes aegypti
… host, virus must disseminate from the mosquito midgut- the initial site of infection following uptake of an infectious bloodmeal- to infect the salivary glands and enter the saliva. The genetic determinants of viral dissemination from the midgut were characterized using the flavivirus yellow …
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Changes in midgut gland morphology and digestive enzyme activities associated with development in early stages of the American lobster Homarus americanus
… system, specifically in relation to the midgut gland (hepatopancreas). in early life history stages of the American lobster Homarus americanus is examined in the present study by means of two separate but complementary approaches. A histological study on the light microscope level details …
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A comparison of the pathological effects on the midgut of the European corn borer treated with Bacillus thuringiensis and Bacillus subtilis
… spores; (2) light microscopic examination of midgut tissues of larvae treated with various concentrations of bacterial spores, and (3) examination of midgut tissues of treated larvae with transmission electron microscopy.The results of the mortality study indicated that B. subtilis did not …
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The Expression of the Transcription Factor Broad and RNA-Binding Factors in the Midgut of the Mosquito Aedes Aegypti During Metamorphosis
… expression in the <em>Ae. aegypti</em> midgut by qPCR, and correlated its expression with that of nine miRNAs and three RNA-binding factors. During midgut metamorphosis the expression of these factors was dynamic and reproducible.</p> <p>To better understand the changes in expression …
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Mechanisms and Genetic Determinants of Alphavirus Infection of Mosquito Vectors
… the particular characteristics of initial midgut infection and dissemination by IE VEEV in Cx. taeniopus. Given previous findings suggesting that the epizootic mosquito vector has a limited number of susceptible midgut cells, I hypothesized that the enzootic vector would not have a …
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<i>Plasmodium</i>-Induced Nitrosative Stress in <i>Anopheles stephensi</i>: The Cost of Host Defense
… ·NO synthase (<i>AsNOS</i>) is induced in the midgut epithelium beginning at 6 h following a <i>Plasmodium berghei</i>-infected blood meal. ·NO reacts readily with other biocompounds forming a variety of reactive nitrogen intermediates (RNIs) that may impose a nitrosative stress. These RNIs are …
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A digestive phospholipase a2 in Midguts of worker honey bees, Apis mellifera : significance in lipid nutrition and health
… addressed by characterizing a digestive PLA2 in midgut preparations of honey bee workers. The honey bee midgut PLA2 is sensitive to reaction conditions, including incubation time, substrate and enzyme concentrations, reaction temperature and pH. It is Ca2+- independent and susceptible to specific …
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Evaluation of Anion Transporters as Potential Target Sites for Insect and Nematode Control: Toxicological, Electrophysiological, and Molecular Studies
… mixed with 100 ppm of DIDS for 6 h decreased the midgut pH by 2 units approximately. All the AT blockers inhibited the growth of larvae (in weight), increased the developmental time, and decreased survival when <i>Ostrinia nubilalis</i> (Hübner 1796) second-instar larvae were fed for seven days. …
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Immunological Crosstalk between Human Transforming Growth Factor-β1 and the Malaria Vector Anopheles stephensi
… active human TGF-β1 (hTGF-β1) persists in the midgut of A. stephensi for up to 48 hours. My data demonstrate that the midgut epithelium recognizes hTGF-β1 as an immunomodulatory cytokine. Specifically, induction of AsNOS by hTGF-β1 occurs in the midgut within minutes of bloodfeeding. Moreover, …
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Induction of Anopheles stephensi nitric oxide synthase by Plasmodium-derived factor(s)
… stephensi nitric oxide synthase (AsNOS) in the midgut epithelium as early as 6 h post-infection and intermittently thereafter. This induction results in the synthesis of inflammatory levels of nitric oxide (NO) in the blood-filled midgut that limit parasite development. However, the …
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Evaluation of Chikungunya Virus Infectious Events in Aedes Albopictus LR and Aedes Aegypti WE Mosquito Vectors
… cells, in ex vivo Ae. albopictus LR mosquito midguts and in vivo in Ae. albopictus mosquitoes. As observed in mammalian cells, we demonstrate that CHIKV infection of mosquito cells depends on acidification of the endosome as indicated by significant inhibition following prophylactic treatment …
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Lyme disease in Indiana : isolation of Borrelia burgdorferi-like organisms from Dermacentor variabilis
… sites in Indiana, from March to October, 1986. Midgut diverticula were examined for the etiological agent, Borrelia burgdorferi, of Lyme disease (LD), using dark-field and immunofluorescence microscopy, and tissue samples cultured in BSK II medium for 7 to 21 days. Spirochetes were isolated in …
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Investigating how Notch, and JAK/STAT Signaling Synergistically Regulate Intestinal Stem Cell Homeostasis Using Engineered Optogenetic Alleles
… I utilize the Drosophila melanogaster midgut as a model system to study stem cell dynamics and more specifically, how Notch and JAK/STAT signaling cooperatively regulate intestinal stem cell turnover. In order to accomplish this, I employed both traditional and optogenetic methods to …
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Xenobiotic detoxification in the navel orangeworm Amyelois transitella (Lepidoptera: Pyralidae)
… the cytochrome P450 genes transcribed in the midgut of the caterpillar. A total of 319 million paired-end reads were sequenced from larval midgut tissue, and these were assembled de novo into 186,000 contigs. Identification of putative P450s in this assembly was carried out using BLAST …
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Proteomic studies of the pheromone biosynthetic pathway in the bark beetle, Ips pini, and molecular modeling of an insect-specific aldehyde oxidative decarbonylase, CYP4G2
… ipdienol. Ipsdienol is produced de novo in midgut tissue and is released through the frass to signal other beetles to initiate a mass attack and colonize a tree. Both feeding and juvenile hormone III (JHIII) treatment stimulate pheromone production. Studies of the genes involved in the …
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Pathogenesis and transmission of Venezuelan equine encephalitis virus
… interactions of VEEV with receptors on midgut epithelial cells determine the ability of a representative epidemic versus a representative enzootic strain to infect this mosquito. In support of this hypothesis, I found that significantly more epidemic VEEV bound to and infected mosquito …
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