Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Microwell array"”.
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Accelerated Engineering of Optogenetic Tools with a High-throughput Microwell Array Platform
Optogenetics has shifted from a cutting-edge technology to a mainstay experimental technique. At its core, optogenetics is the use of light sensitive proteins to report or control biological activity. The unique spatiotemporal precision afforded by optogenetic tools has propelled the technique to …
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A microwell array cytometry system for high throughput single cell biology and bioinformatics
… PDMS device consisting of one or more microwell arrays, each with associated alignment and identification features, and 2) a suite of custom software tools for automated image processing and data analysis. The system has a number of significant advantages over competing technologies …
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Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… detect them with the help of a digital microarray and a smart actuation device for sealing the microwells. The chip achieves the EV quantification by digital Enzyme-Linked Immunosorbent Assay (ELISA) and enzymatic signal amplification. Specific areas of glass slide beneath the detection area …
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Microfluidic cell culture chambers with nanoporous walls for chemical communication
… dimethacrylate) (HEMA-EDMA), to fabricate a microwell array with 105 individual culture chambers. Pore size of HEMA-EDMA was confirmed by ESEM imaging to be less than 200 nm, adequate for isolating all identified bacteria. We have video-recorded fluorescence labeled Escherichia coli swimming …
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Cell-cell and cell-medium interactions in the growth of mouse embryonic stem cells
… for studying intercellular signaling - the nDEP microwell array and the Bio Flip Chip (BFC). We used the BFC to show that cell-cell interaction improves the colony-forming efficiency and the self-renewal of mouse ESCs. Further, we demonstrate that the interaction is at least partly diffusible. In …
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Spatially mapped gene expression analysis from tissue
… technique uses a micro-fabricated chip with an array of micro-wells with knife-like sharp well edges to assist in division of a tissue cryosection into small pieces in a process we call ""tissue pixelation"". The array consists of over five thousand 100um (side length) pyramidal wells with a …
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Engineered 3D microenvironments to understand and direct liver progenitor and cancer cell fate
… fate. The other system utilized a tunable microarray system to create geometrically controlled hepatocellular carcinoma spheroids and 3D cancer co-culture spheroids with relevant niche cells, showing how manipulating the aggregate size, shape and potential co-culture changed cell behavior and …