Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Microwell"”.
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Accelerated Engineering of Optogenetic Tools with a High-throughput Microwell Array Platform
Optogenetics has shifted from a cutting-edge technology to a mainstay experimental technique. At its core, optogenetics is the use of light sensitive proteins to report or control biological activity. The unique spatiotemporal precision afforded by optogenetic tools has propelled the technique to …
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A microwell array cytometry system for high throughput single cell biology and bioinformatics
… PDMS device consisting of one or more microwell arrays, each with associated alignment and identification features, and 2) a suite of custom software tools for automated image processing and data analysis. The system has a number of significant advantages over competing technologies …
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Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… and a smart actuation device for sealing the microwells. The chip achieves the EV quantification by digital Enzyme-Linked Immunosorbent Assay (ELISA) and enzymatic signal amplification. Specific areas of glass slide beneath the detection area of the chip coated with anti-CD81 antibodies are …
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Microfluidic processes to create structured microparticle arrangements and their applications
… of a new microfluidic platform, porous microwell arrays, to create structured large-scale microparticle arrays using a scaling theory, which is a function of particles' characteristics. Lastly, we demonstrate the potential of generated particle arrays in various bioengineering …
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The Influence of Human Umbilical Vein Endothelial Cells in the Formation of Glioblastoma Spheroids in Three-Dimensional Microwells
… GBM cells with endothelial cells in the hydrogel microwells would mimic the in vivo microenvironment of tumor cells. Thus, we could provide beneficial 3D in vitro co-culture models for GBM treatment with oncolytic adenovirus, Delta-24-RGD (Δ24RGD). Therefore, in the first part of this study, we …
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Development of High-Throughput Platforms for Single-Cell Analysis
… tools that rely on microfabricated arrays of microwells. The first platform merges fluorescence cytometry with label-free profiling of the small molecule composition of tens of thousands of cells based on matrix assisted laser desorption/ionization (MALDI) mass spectrometry. We evaluated …
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A noninvasive screening tool for cell fate determination using raman spectroscopy and machine learning in tissue engineering and microbiology applications
… be noninvasively tracked. We developed a novel microwell platform that facilitates repeatedly relocating the same cells, and noninvasively tracking their differentiation over time in response to 3D interactions with bioinspired hydrogels. We first established that Raman spectroscopy accurately …
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Investigating the role of geometry, mechanics, and microenvironmental cues on liver progenitor cell differentiation
… describe the development of a hydrogel-based microwell platform, which I used to produce arrays of 3D, multicellular liver progenitor cell microtissues in constrained geometries. These generated distinct mechanical profiles which I modeled with finite element method software, which was …
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Microfluidic cell culture chambers with nanoporous walls for chemical communication
… dimethacrylate) (HEMA-EDMA), to fabricate a microwell array with 105 individual culture chambers. Pore size of HEMA-EDMA was confirmed by ESEM imaging to be less than 200 nm, adequate for isolating all identified bacteria. We have video-recorded fluorescence labeled Escherichia coli swimming …
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Developing electrochemical methodologies towards addressing challenges in flow energy conversion technologies
… diagnostic capabilities, a modified flooded microwell SECCM approach was developed that overcame meniscus instability issues in alkaline media to enable screening of bimetallic catalyst arrays, demonstrating superior performance of Au-Pd systems over other bimetallic combinations for glycerol …
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Optimization of Microfluidic, Point of Care, Flow-Through, Bead-Based Microarrays: Towards Affordable Healthcare
… times are short, the geometry of the bead microwell design is optimized to improve the fractional capture efficiency of analytes by the beads in flow through microcontainers. Finally, to improve the imprecision performance in bead-to-bead signal variation within the microarray, exploration …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
… mixture to create substrates with circular microwells. Finite element modeling (FEM) was used to show that microwell topography was a result of backfill contraction during exposure. The FE model was then used to make predictions for further substrate design. The techniques presented in this …
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Single-electron transport and electron-phonon interactions in graphene heterostructured self-assembled molecular solid-state devices
… single-electron phenomena in finger-design and microwell devices, such as the Coulomb staircase, accompanied by three distinct types of negative differential resistance, hysteresis, and random telegraph noise. Devices were fabricated using 5 nm and 2 nm PbS nanocrystals attached to SAMs derived …
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Cell-cell and cell-medium interactions in the growth of mouse embryonic stem cells
… for studying intercellular signaling - the nDEP microwell array and the Bio Flip Chip (BFC). We used the BFC to show that cell-cell interaction improves the colony-forming efficiency and the self-renewal of mouse ESCs. Further, we demonstrate that the interaction is at least partly diffusible. In …
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A Time-Gated Fluorescence Instrument and Its Applications Using Mn-Doped I(II)-III-VI Nanocrystal Probes
… 354 pg/mL and CuZnInS 2.9 ng/mL) and a simple microwell surface-based biotin-avidin binding assay (316 ng/mL and 1025 ng/mL). Based on these results, Mn:AgZnInS/ZnS nanocrystals alone were utilized in a magnetic bead-based sandwich immunoassay for the Burkholderia pseudomallei capsular …
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Determination of Pancreatic and Salivary Amylase By Enzyme Immunoassay and Their Prevalence in Hyperamylasemic Patients
… each isoenzyme.</p> <p>A sensitive and specific microwell assay based on sandwich technique for the quantitative determination of the p-amylase and s-amylase was developed. Microwells were utilized for the solid-phase immobilization of the amylase inhibitor cycloheptaamylose (CHA) which was …
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Physiological response of bacillus licheniformis NCIMB 8874to oligosaccharide elicitors
… cells with oligosaccharide elicitors in microwell plates resulted in changes in Ca2+ influx. The highest influx was 9.2 % greater in samples incubated with MO and OG compared to the control. Addition of ionomycin (positive control) and verapamil (Ca+2 channel blocker) to the cultures of …
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Single-molecule diagnostics to support curative interventions for tuberculosis and HIV
… is limited because it relies heavily on advanced microwell and imaging technology. We developed a new digital ELISA platform, called Molecular On-bead Signal Amplification for Individual Counting (MOSAIC), that performs the final readout step with a flow cytometer, bringing digital ELISA within …
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Microbial dynamics of bioleaching consortia
… included batch small-scale tests in 12-well Microwell plates (MWPs) with only an iron source, tests in MWPs with both an iron and sulfur source, and a semi-continuous test in 1 L bioreactors with a pyritic tailings material. In all studies, increasing concentrations of Ni2+ and Cr3+ were …
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Active cellular flows: biological networks, cytoplasmic streaming and swimming bacteria
… above rigid surfaces, but in a cylindrical microwell with top-bottom asymmetric boundary conditions, they segregate into two populations of different sizes at opposite flat boundaries, and the smaller population reverses rotational direction. This motivates a flow singularities model for the …
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