Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"Microtubule Dynamics"”.
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Microtubule Dynamics in Neuronal Morphogenesis
Microtubules in neurons provide structural stability, and also provide a transport route for cellular cargo carried by motor proteins. Exactly how microtubules are organized and maintained in neurons is not clear, but microtubule organizing centers (MTOCs) in neurons are not required as in …
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Mechanistic Analysis of Microtubule Dynamics and Regulation
Microtubules are critical components in a cells cytoskeletal network, known to form the mitotic spindle which allows for chromosome segregation and cell division and organizing the cytoplasm of non-dividing cells. The quick reorganization of the cytoskeleton relies heavily on the underlying …
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Modelling and image processing of microtubule dynamics and organisation
Microtubules, dynamic protein polymers, form networks that are essential for intracellular organisation. Involved in many cellular processes that are vital in development and homeostasis, improper regulation of the microtubule network is implicated in various diseases. This work addresses the …
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DECIPHERING THE FUNCTION OF HURP IN REGULATING MICROTUBULE DYNAMICS AND CHROMOSOME MOVEMENT
The regulation of mitotic spindle dynamics is important for faithful chromosome segregation. HURP is an important microtubule-associated protein for stabilizing and bundling the mitotic spindle. Depletion of HURP delays the mitotic progression, but the underlying mechanism remains unknown. Our …
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INVESTIGATION OF ALPHA-SYNUCLEIN/TUBULIN INTERACTION IN PRESYNAPTIC MICROTUBULE DYNAMICS IN DOPAMINERGIC NEURONS
… pathology, PD lacks these findings. Nonetheless, microtubule (MT) dysfunction has recently emerged as a putative cause of the selective damage of the striatal DA synapses and consequent reduction in dopamine release that characterize PD clinical symptoms. It was only recently when dynamic MTs were …
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The identification of the Tao-1 kinase as a key regulator of microtubule dynamics
… protein leads to the stabilisation of microtubules and to the formation of long microtubule-based protrusions, whereas the overexpression of Tao-1 destabilizes microtubules. I also showed in this study that Tao-1 acts independently of Par-1 and Tau in this process. Instead, Tao-1 …
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Recombinant αβ-Tubulin and a Simple Computational Model Shed Light on the Molecular Mechanisms of Microtubule Dynamics
Microtubules (MTs) are essential to all eukaryotic organisms. They help segregate chromosomes and organize the cytoplasm. MTs are hollow barrels of the protein αβ-tubulin that exhibit a non-equilibrium behavior called dynamic instability: the stochastic switching of single polymers from a state of …
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Poxvirus A51R Proteins Directly Bind to Microtubules to Antagonize Microtubule Dynamics, Intracellular Transport, and a Cell-intrinsic Antiviral Response
Viruses often manipulate the microtubule (MT) network to facilitate their replication inside host cells. Poxvirus A51R proteins have been shown to colocalize with, and bundle, MTs, but the mechanisms of their MT interactions remained unclear. Additionally, vaccinia virus (VV), a model poxvirus, …
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A NOVEL, RAS-INDEPENDENT ROLE FOR NF1 IN MICROTUBULE DYNAMICS AND DAMAGE REPAIR DICTATES SENSITIVITY TO T-DM1 IN HER2 POSITIVE BREAST CANCER.
… co-purify with tubulin and to co-localize with microtubules (MTs); two regions in NF1 have been proposed to mediate this interaction. The physiological relevance of this interaction remains uncertain, and NF1 is not generally included among bona fide Microtubule-Associated Proteins (MAPs). Our …
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Kinetochore Structural Plasticity and its Regulation in <i>Saccharomyces cerevisiae</i>
… complex that connects the chromosome with microtubule and pulls the chromosome during chromosome segregation. In yeast, a single kinetochore assembles on a Cse4 nucleosome and attaches to a single microtubule, making it an ideal system for studying the architecture of …
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Xenopus DPPA2 is a Direct Inhibitor of Microtubule Polymerization Required for Nuclear Assembly
… We show that Dppa2 directly depolymerizes microtubules, and must be localized on DNA to tune local microtubule dynamics for proper nuclear assembly. In the absence of Dppa2, excess microtubules compromise nuclear shape, while ectopically abolishing microtubules retards nuclear expansion. …
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Targeting the Colchicine Binding Site on Tubulin to Overcome Multidrug Resistance and Anticancer Efficacy of Selective Survivin Inhibitors
… successis attributed to their ability to target microtubule dynamics and disrupt critical cellular functions including cell signaling, motility, intracellular trafficking, and mitosis. Interference with microtubule dynamics consequently disrupts mitotic progression and ultimately leads to …
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Examining Mechanisms Regulating Microtubule Assembly and Function
<p>Microtubules are cytoskeletal polymers assembled from α and β tubulin subunits that function in essentially all cellular activities. Microtubules can act as "tracks" for intracellular cargo transport, are required for cilia- and flagella-based motility, and establish cell morphology in …
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Non-Enzymatic Activity Of The Alpha Tubulin Acetyltransferase Alpha-Tat1 Differentially Alters The Stability Of Dynamic And Stable Microtubules
… network of both long-lived and dynamic microtubules that provide structural stability for the boutons while also allowing for their growth and remodeling. However, a molecular-scale mechanism that explains how neurons appropriately balance these two microtubule populations remains a …
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The RASSF1A Tumor Suppressor Regulates a Cascade of Oncogenic Signals That Are Restrained by G1 Checkpoint Mechanisms
… regulate apoptosis, cell cycle progression, and microtubule dynamics. Disruption of these processes by RASSF1A loss may disrupt cellular integrity and promote oncogenesis. I found that RASSF1A depletion elevated oncogenic signaling pathways; however, RASSF1A depletion also induced cell cycle …
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Design and Synthesis of CDK-5 and MEK-5 Inhibitors and Synthesis towards Tubulysin Analogs
… is associated with hyperphosphorylation of the microtubule-associated protein tau, formation of neurofibrillary tangles, and possibly an acceleration of the neurodegenerative progression of Alzheimer's disease. C6-O linked benzimidazoles and C-4 benzamide and phenylacetamide benzimidazoles based …
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Regulation of chromosome attachment and dynamics by Saccharomyces cerevisiae kinetochores
… complexes that bind centromeric DNA to the microtubules of the mitotic spindle and mediate chromosome movement throughout the cell cycle. The proteins that regulate both force generation at kinetochores as well as and the cell-cycle-dependent changes in kinetochore architecture are largely …
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The Role of LIM Kinase 1 and its Substrates in Cell Cycle Progression
<p>LIM Kinase 1 (LIMK1), a modulator of actin and microtubule dynamics, has been shown to be involved in cell cycle progression. In this study we examine the role of LIMK1 in G1 phase and mitosis. We found ectopic expression of LIMK1 resulted in altered expression of p27Kip1, the G1 phase Cyclin …
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Microtubule Organisation in Normal and Colon Cancer Cells
… function," with" the" rearrangement" of" the"microtubule" cytoskeleton" on" differentiation" critical" for" the" establishment" of" polarity." "During" this" process,"microtubules" reorganise" to" form"a"stabilised"array"of"apicoNbasal"bundles"via"a"release"and"capture"mechanism."" …
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Investigating the Role of Insulin-Like Signaling in Paclitaxel-Induced Nociceptive Hypersensitivity using Drosophila melanogaster
… (PTX), a chemotherapeutic that alters microtubule dynamics, is known to induce nociceptive hypersensitivity and sensory neuron damage in human patients and in Drosophila larvae. We used a modified feeding protocol to establish a genetically tractable Drosophila model of PTX-induced …
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