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Showing 1 to 20 of 107 for “"Microsomes"”.
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Content and Activity of Cytochrome P450 3A in Rat Brain Microsomes and Mitochondria
<p>The potential contribution of CYP3A in brain microsomes and mitochondria is crucial to designing appropriate therapeutic strategies and preventing toxicities. Until recently, there has been limited data reported in the literature with respect to CYP3A activity and its expression in the brain …
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Role of Cytochrome P-450 in Methemoglobin Formation by Primaquine with Hamster Liver Microsomes
… red blood cells with primaquine, hamster liver microsomes and a NADPH-generating cofactor mixture. In the control flasks, the cofactor mixture was omitted. After the incubations, the red blood cells were separated from the other components of the incubation flask by differential centrifugation …
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Propoxyphene, Norpropoxyphene, and Proadifen (SKF-525A) Are Mechanism Based Inhibitors of CYP3A4, CYP3A5, and CYP3A in Human Liver Microsomes
… CYP3A4(+b5), CYP3A5(+b5) and pooled human liver microsomes. Time-dependent and concentration-dependent loss of activity of CYP3A was measured by formation of testosterone product. Propoxyphene and norpropoxyphene exhibited the greatest inhibition with CYP3A in human liver microsomes, followed by …
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The Effect of Different Dietary Lipids Upon Major Lipid Classes and Long-Chain Fatty Acyl Coenzyme-a Synthetase of Rat Liver Microsomes
Made available in DSpace on 2015-05-13T15:26:55Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7709117.PDF: 4695992 bytes, checksum: 2373c4fa8e38e6548835a1e90c3a2be8 (MD5) Previous issue date: 1976
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Determination of Absolute Protein Content of Hepatic CYP4F Enzymes in Human Liver Microsomes Using LC/MS/MS Methodology and Comparison with Immunoquantification and Enzyme Activity
The purpose of the work presented herein was to determine the absolute protein content of multiple cytochrome P450 (CYP) enzymes in individual donor human liver microsome (HLM) samples. The CYP4F subfamily of enzymes have recently been identified to be involved in the metabolism of endogenous …
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Effects of Pycnogenol on Microsomal Cytochrome P450 Activity and Apoptosis
… NNK is a lung carcinogen. Lung and liver microsomes from 6 mo and 20 mo old male F344 rats were incubated with <sup>3</sup>H-NNK in the presence of pycnogenol and following intragastric administration of pycnogenol. Pycnogenol inhibited the in vitro metabolism of NNK in both liver and lung …
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Isoflurane : interaction with hepatic microsomal enzymes
… with cytochrome P-450 in rat and human hepatic microsomes and the Δ6- and Δ5-desaturases in rat hepatic microsomes. The interaction of isoflurane with cytochrome P-450 results in its metabolism to fluoride ion and organofluorine metabolites. The cytochrome P-450 isozymes catalysing the …
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Studies on Ribosome-Membrane Interaction
… chains were terminated by incubating rough microsomes in a medium optimal for amino acid incorporation, a subsequent incubation in a solution containing 0.5 M KCl and 0.0025 M MgCl<sub>2</sub> resulted in a partial detachment of ribosomes, as 40 S and 60 S particles. b) Solutions containing …
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Effect of cell- and species-differences in the expression of drug metabolism enzymes on chemical-induced injury to the rat and human kidney
… Pyridine induced CYP2E1 in both liver and kidney microsomes but not in rPT and rDT cells. Clofibrate induced CYP4A and CYP2B only in liver and kidney microsomes and also CYP2E1 and CYP2C11 in kidney microsomes. The GST isoforms responsible for GSH conjugation of Tri were also determined. Pyridine …
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The biological properties of three trichothecene mycotoxins produces by fusaris
… The high deacylation rate catalysed by rat microsomes correlated with the low sensitivity of this species to T-2 toxin, whereas the low deacylation rates with cat and monkey microsomes agreed with their high sensitivity. In contrast to this, the apparently high toxicity of T-2 toxin to …
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Effect of berberine on in vitro metabolism of sulfonylureas: a herb-drug interactions study
… sulfonylureas were co-incubated with rat liver microsomes in the presence of freshly prepared NADPH regenerating system. The metabolites of berberine and sulfonylureas were analysed using liquid chromatography with high resolution mass spectrometry operated in the positive ion mode. The role of …
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Vincristine Metabolism and the Role of CYP3A5
… models: cDNA-expressed enzymes, human liver microsomes, and human hepatocytes. With these models, the major CYP metabolite of vincristine, M1, was identified and extensively characterized. The rates of M1 formation in the cDNA-expressed enzyme models were at least 7-fold higher with CYP3A5 …
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Hepatic microsomal and peroxisomal docosahexaenoate biosynthesis during piglet development
The roles of peroxisomes and microsomes on the biosynthetic pathway for docosahexaenoic acid (DHA) from [Alpha]-linolenic acid (ALA) were investigated. Microsomes and peroxisomes were prepared from livers of fetal and neonatal piglets by a combination of differential and gradient layer …
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Characterisation of mammalian cytochrome P450 enzymes by their metabolic activities.
… to microsomal harmine metabolism. 1) Human liver microsomes from 16 different individuals metabolised harmine to identifiable metabolites, which were detected by normal phase HPLC following incubation with 3[H]-harmine in the presence of NADPH and oxygen. Harmol was identified as the major …
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Quelques aspects de la synthèse de l'hème dans la glande surrénale
… de la glycine [3H] dans les mitochondries et les microsomes de la glande surrénale du rat ; nous avons aussi regardé l'effet de l'ACTH sur la biosynthèse de l'hème. Des préparations d'homogénats de la glande surrénale ont été incubées "in vitro", en présence des substrats radioactifs. L'hème a été …
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The effect of membrane lipid hydrogenation on drug metabolising activity
… would be used for the hydrogenation of PC and microsomes. The extent of hydrogenation was determined using gas chromatography (GC). GC traces of linoleic acid samples showed evidence of hydrogenation occurring through the presence of saturated forms namely oleate and stearate. The GC trace of …
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Studies on intracellular calcium pools in a pancreatic cell line
… Ca²⁺ could be actively accumulated into the microsomes driven by an ATP-dependent Ca²⁺ -ATPase. Addition of IP₃ maximally caused a 20% release of actively accumulated Ca²⁺ which was completely blocked by heparin, an antagonist of the IP₃ receptor. Extravesicular Ca²⁺ produced an inhibition of …
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