Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Microscopy, Fluorescence"”.
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Discrimination Of Automobile Carpet Fibers Using Multiple Analytical Techniques And The Subsequent Creation Of A Searchable Data
… These techniques included polarized light microscopy, fluorescence microscopy, microspectrophotometry, Fourier-transform infrared spectroscopy, microtomy (cross section analysis), dye extraction and liquid chromatography-mass spectrometry. Based on the information obtained from these …
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Homeostasis and volume regulation in the Plasmodium falciparum infected red blood cell
… the intraerythrocytic cycle using confocal microscopy. Fluorescence lifetime imaging and electron probe X-ray microanalysis were applied for the quantification of intracellular haemoglobin and electrolyte concentrations. The cytomechanical properties of uninfected and infected red cells were …
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Detection of environmental pollution (radionuclides and heavy metals) using microorganisms.
… by the optical techniques,including fluorescence microscopy, fluorescence spectroscopy, optical density OD600 and UV-visible spectroscopy and was evaluated or estimated according to or attributed to the time of exposure to gamma radiation, which are emitted from CO57 radiation source. …
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Microbiology and cell biology of the interaction between Listeria monocytogenes and Acanthamoeba spp.
… and amoebae. Bacteriological methods and microscopy (fluorescence, TEM and phase contrast) were used to track the fate of internalized bacteria. A vector that allowed GFP expression under control of the prfA promoter was used to assess the expression of Listeria virulence genes within …
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Quantitative Analysis of the Spatial and Temporal Organization of Cell Surface Receptors
Appendix B contains a previously published article, which is referenced below as a related citation.
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Alternative signal transduction for functional nucleic acid sensors
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Computational methods for fast nonlinear optical microscopy of label-free metabolic dynamics in live organisms
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01
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Intracellular labeling of live cells via transient membrane permeabilization for fluorescence and super resolution microscopy
Fluorescence imaging of intracellular proteins is often achieved by using transfection-induced expression of fluorescent protein. This can potentially impose artifacts such as loss of function or over-expression of target proteins. Direct labeling of the intracellular protein is an alternative to …
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Resistance Responses in Pseudomonas aeruginosa PAO1 in Response to Surface Predation by Myxococcus xanthus DK1622
… study employs multi-sample time-lapse confocal microscopy to provide a clearer picture of this unique resistance response. Mixed cell assays showed statistically significant rejection of live prey and an increase in dead prey at the cellular level. Timelapse videos showed that ripple formation …
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Using Deep Learning to Extract Multicellular Aggregation Features of Myxococcus xanthus
… adversarial network (GAN) that synthesizes fluorescence microscopy images from phase-contrast images. This approach combines the ease of phase-contrast acquisition with the quantitative advantages of fluorescence imaging, improving aggregate segmentation and enabling accurate analysis of …
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Label-free multiphoton microscopy for imaging transient metabolic dynamics in living cells and tissue
… one such tool through the use of two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of reduced nicotinamide adenine dinucleotide (NADH). This autofluorescent co-enzyme is involved in both aerobic and anaerobic metabolic processes which can be differentiated utilizing this advanced …
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Graphene Microelectrode Arrays to Combine Electrophysiology with Fluorescence Imaging of Amyloid Proteins
… Two techniques used in amyloid research are fluorescence microscopy, to map the protein location and aggregation state, and electrophysiology, to examine the effect of the proteins on neurons. To enable these techniques to be combined, a transparent graphene microelectrode array (MEA) was …
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Processive Acceleration of Actin Barbed End Assembly by N-WASP
Actin-based cell motility plays crucial roles throughout the lifetime of an organism. The dynamic rearrangement of the actin cytoskeleton triggers a plethora of cellular processes including cellular migration. Neural Wiskott Aldrich syndrome protein (N-WASP) is involved in transduction of signals …
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Characterization of erythrocyte membrane domains using fluorescence digital imaging microscopy
Fluorescence digital imaging microscopy was used to characterize the lateral distribution of the lipid and protein components of fluorescently labeled erythrocyte membranes. Experiments with erythrocytes and erythrocyte ghosts labeled with fluorescent phospholipids showed that the lipids are …
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Sviluppo e ottimizzazione di tecniche di bioimaging ad alta risoluzione spaziale basate su strategie di rilevamento innovative
La microscopia confocale e la microscopia a super-risoluzione, note anche come microscopia a scansione di immagini (ISM), sono due potenti strumenti per l'imaging ottico ad alta risoluzione, ampiamente utilizzati nella ricerca biomedica. Tuttavia, entrambe le tecniche sono soggette a limitazioni di …
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Identifying vaping hazards through the evaluation of exposure methods and cell culture models for e-liquid mixtures.
Vaping has become a popular method of engaging in “smokeless” socialization since the introduction of e-cigarettes in the United States tobacco market. In 2007, e-cigarettes were presented as portable vaping devices serving as alternatives to traditional tobacco cigarettes. Due to the evolution of …
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Measurements and analyses of transposable element activity inside living cells
… to quantify its activity in single cells via fluorescence microscopy. The system can reveal single events, cell-to-cell variations, and temporal and environmental variabilities in real time and individual living cells. With this system, we characterize the relationship of the level of …
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Development of Novel Labelling Strategies for Imaging in Nanoscale Topography
… scales. 3D single molecule localisation microscopy (3D-SMLM) techniques such as the double helix point spread function (DHPSF) enable the visualisation of these systems on the required spatial scales and take into account the inherently 3D nature of biology. However, the addition of axial …
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
Over the past few decades, fluorescence microscopy has proven to become the most widely used imaging technique in the field of life sciences. Unfortunately, all classical optical microscopy techniques have one thing in common: their resolution is limited by the diffraction. Thankfully, due to the …
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Advances in volumetric super-resolution microscopy and single-particle tracking
Single-molecule localisation microscopy (SMLM) has enabled optical microscopy to probe biological structures and processes at the nanometer scale. To accommodate the inherently 3D nature of biological structures, 3D-SMLM techniques---such as the astigmatic and double-helix point spread functions …
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