Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 34 for “"Micropipette"”.
-
Development of a novel micropipette aspiration-based force sensing technique to measure single cell sliding friction
… Thus, we propose an instrument based on the micropipette aspiration technique to solve this problem. In this setup, a highly sensitive friction force sensor is made out of a long, thin, and hollow micropipette that deflects as a function of the tangential force experienced by a cell, …
-
A Clearer Picture of Musculoskeletal Disorders of the Knee and Thumb Through the Lens of Image-Based Finite Element Analysis
… work was to develop a modular MRI-compatible micropipette simulator that could be used for MRI-based FEA of the basilar thumb joint. A modular MRI-compatible micropipette simulator addresses several limitations of pipetting studies, and it can be used to isolate micropipette design features to …
-
Universal robot for automated microinjection with applications in transgenesis and cryopreservation
… organisms at a microscopic level using a glass micropipette. It is a widely utilized technique with a wide range of applications in both fundamental research and clinical settings. However, microinjection is an extremely laborious and manual procedure, which makes it a critical bottleneck in the …
-
Multiphase, Multicomponent Systems: Divalent Ionic Surfactant Phases and Single-Particle Engineering of Protein and Polymer Glasses
… on work done previously in the lab by using the micropipette technique to study two-phase microsystems. These microsystems consist of a liquid droplet suspended in a second, immiscible liquid medium, and can serve as direct single-particle studies of drug delivery systems that are formed using …
-
Rapid single-cell electroporation for labeling organotypic cultures
… load, clean, and accurately position a glass micropipette electrode into tissue culture for single-cell electroporation is proposed. The system will significantly increase the number of different labeling agents that can be introduced into a single tissue culture per unit time. This in turn, …
-
Automation of Single Cell Micromanipulation and Microsurgery for Blastocyst Embryo Biopsy
… the sample aspiration and track it inside the micropipette. The fourth step involves separating the sample from the embryo and retrieving it. A vision feedback control system is developed to move the embryo and the micropipette while a multi-pulses laser ablates the sample to separate it. For …
-
Precision pipetting and crack-free colloidal assembly
… prototype device for this universal micropipette concept is reported, along with practical considerations for implementation and possible commercialization. Second, a direct-write method to build freestanding colloidal structures via capillary-driven self-assembly from a needle is …
-
Axon Guidance Autonomy and Tensional Requirement for Synaptic Function: Is Learning a Forced Response
… presynaptic site normally. However, if held by a micropipette that resupplies mechanical tension, the growth cones' ability to localize synaptic vesicles is restored. Our results demonstrate functional autonomy of axonal growth cones as they navigate and initiate synaptogenesis, while implicating …
-
Harmonic Acoustics for Single Cell Manipulation
… atomic force microscopy, optical tweezers, and micropipette aspiration, suffer from low throughputs, insufficient repeatability over the single cell assay, and physical contacts that can negatively influence testing results. On the other hand, conventional acoustic tweezers can manipulate cells …
-
Multilevel, subdivision-based, thin shell finite elements: development and an application to red blood cell modeling
… constant-volume deformation of a red blood cell: micropipette aspiration and point load application.
-
Nano-pipette as nanoparticle analyzer and capillary gated ion transistor
… nanoparticles enter the nanopore of a pulled micropipette, producing a clear translocation signal. Pulled borosilicate micropipettes with opening 50 ~ 350 nm were used as the detecting instrument. This method provides a direct, fast and cost-effective way to characterize inorganic and organic …
-
Cadherin recognition and specificity
… First, the bond properties were studied with the micropipette manipulation method. The results revealed that cadherin binding exhibits biphasic kinetics. Further studies with domain deletion mutants showed that the EC1 domain of cadherin extracellular region is responsible for the first phase of …
-
Cellular responses to mechanical stresses applied via magnetic manipulators
… force microscopy, optical and magnetic tweezers, micropipette aspiration and other techniques have been used to explore and develop physical models of the cell. By using these techniques, the basic physical response of a cell to mechanical forces has been analyzed, yet the reported cell moduli in …
-
A cylindrical specimen holder for electron cryo-tomography
… process, involving carbon deposition on glass micropipette templates and subsequent removal of the glass. Tube diameters are typically a few hundred nanometres, with a wall thickness of 10-20 nm. To make frozen-hydrated specimens, the tubes are filled with an aqueous sample and then …
-
A cylindrical specimen holder for electron cryo-tomography
… process, involving carbon deposition on glass micropipette templates and subsequent removal of the glass. Tube diameters are typically a few hundred nanometres, with a wall thickness of 10–20 nm. To make frozen-hydrated specimens, the tubes are filled with an aqueous sample and then …
-
A Multielectrode Microcompartment Platform for Signal Transduction in the Nervous System
… electrophysiological recordings (via a patch micropipette) are made routinely to record action potentials and ion channel activity in neurons. Moreover, Campenot chambers (traditional compartmented culture systems) have been used for the last thirty years to study the selective application of …
-
Mechanochemical pattern formation in the cellular actomyosin cortex
… external geometrical deformations by combining micropipette aspiration with live fluorescence imaging. In Chapter 4, I describe the infrared spectroscopy setup built in the pursuit of uncovering the properties of fluorescent markers. Taken together, the work in this thesis outlines a …
-
Kinetic analysis of Fcγ receptor and T cell receptor interacting with respective ligands
… and glycosylation on ligand binding. We used a micropipette adhesion frequency assay to demonstrate that the anchor length affects the forward rate and affinity of CD16s for IgG in a species specific manner, most likely through conformational changes. Receptor glycosylation dramatically reduced …
-
Blockade of Acetylcholine Receptors by Cobra Toxin Electrophysiology and Pharmacology
… For the frog sartorius myoneural junction, micropipette electrodes and on-line computer were used to show that the toxin specifically and irreversibly inactivates postsynaptic acetylcholine (ACh) receptors. Raja and Torpedo electroplaques also show nicotinic pharmacology and were similarly …
Page 1 of 2