Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Micropatterning"”.
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Weak polyacid brushes : synthesis, swelling behavior, complex formation and micropatterning
In this work, densely end-grafted polymer monolayers, so-called "polymer brushes", were covalently attached to solid planar surfaces and investigated with respect to their properties in contact with aqueous solutions of varying composition. <br> <br>The synthesis of these polymer brushes was …
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Leveraging cell micropatterning technology for rapid cell-based assessment of chemical toxicity and population variation in toxicity susceptibility
With the advent of combinatorial chemistry, the number of novel synthetic chemicals has skyrocketed over the past three decades, bringing about tremendous advances in medicine and material science. At the same time, the massive libraries of existing chemicals coupled with the unprecedented rate of …
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Synthetic In Vivo Reconstitution of Asymmetric Cell Division
… The first of these techniques relies on protein micropatterning, which is the process by which proteins can be ‘printed’ onto a surface. For successful establishment of this assay, a new system of micropatterning, based on ‘anchoring’ proteins to Fibrinogen (a large glycoprotein complex), was …
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In vitro reconstitution of Central Spindle Motility
… vitro reconstitution using purified proteins and micropatterning. To address how different endosomes segregate on an asymmetric microtubule track I utilised knock-in Rab-GFP lines and internalised labelled anti-delta antibodies. Remarkably, a class of late endosomes containing anti-delta …
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In situ magnetoresistance measurements during patterning of spin valve devices
… layers. The increased coercivity induced by micropatterning changed the natural similarity of the magnetic layers and the structure exhibited a small spin valve response. These initial measurements were carried out with separate milling and electrical characterisation steps. However, it was …
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Characterization and patterned polymer films from a novel self-assembly process
… for the deposition of SAMp films and for micropatterning of organic surfaces based on our results. Such results significantly extend the application of SAMp films since the sulfonate-functionalized surface can be introduced into the surfaces of aromatic polymers, metals, ceramics, …
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Fabrication of Micropatterned Hydrogels for Cell Culture
… is described for viscoelastic materials. Micropatterning methods of hydrogels are investigated in variety of approaches. How the patterned surfaces affect cell behaviour is discussed in our literature review. From the experimental results, agarose and polyethylene (glycol) diacrylate are …
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Cardiac organoid technology and computational processing of cardiac physiology for advanced drug screening applications
… </p> <p>Cardiac organoids, generated via micropatterning techniques, were explored to determine how controlling engineering parameters, specifically the geometry, direct tissue fate and organoid function. The advantage of cardiac organoid models is the ability to recapitulate and study …
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Biomaterials for the extracellular control of mesenchymal stem cell pro-angiogenic potential
… on matrix composition. In Chapter 3 we use micropatterning to reveal how changing cell shape (modulating cellular cytoskeleton, focal adhesions and contractility) can modulate not only the pro-angiogenic potential of MSCs but their phenotype and epigenetic state. We develop a biocompatible …
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Micropatterned cell arrays for detecting DNA damage
… whether the COMET assay can be improved by micropatterning adherent cells prior to agarose embedding. Hepatocytes were chosen as a model cell type and x-ray radiation was chosen as a model DNA damaging agent. In order to establish the feasibility of measuring x-ray induced damage on …
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Polyelectrolyte multilayers (PEM) in micro / nanofluidics for novel BioMEMS platforms
… excellent selective antibiofouling results for micropatterning of bacteria.
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From iPSCs to Engineered Heart and Liver Models: Modulating Genetic and Extracellular State for Maturity
… Additionally, we engineered a soft-lithographic micropatterning platform that generates aligned, fibroblast-supported cardiac tissues in multi-well plates. Epicardial-derived iPSC fibroblasts produced the strongest maturation effects, enabling fully patient-specific tissues suitable for disease …
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Microfabrication of conductive polymer nanocomposite for sensor applications
… printing and laser ablation assisted micropatterning processes. Prototype devices including large-strain strain sensor and highly-sensitive pressure sensor were demonstrated showing distinct advantages over existing technologies. The polymer nanocomposite used in this work comprised …
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Engineering Liver and Cardiac Models for Drug Development and Regenerative Medicine
… that hinders their utility. Strategies such as micropatterning, coculture with organ-specific fibroblasts, and organoid formation help maintain the phenotype, function, and stability of various cell types. In this proposal we aim to develop models to overcome the barriers in tissue engineering …
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A Platform for Analyzing Force Sensitivity and Multivalency in Actin Networks
… linker complexes and combining them with photo-micropatterning of myosin motor proteins to produce an in vitro reconstitution platform for examining how force impacts the behavior of ABPs within multi-filament assemblies. Our system enables monitoring dozens of actin networks with varying …
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Photonic Properties of Self -Assembled Organic Materials: Supramolecular Films, and Nanoribbon -Templated Gels and Polymers
… patterned substrates, suggesting that substrate micropatterning enhances overall polarization in these supramolecular films. DRC molecules contain covalently connected dendritic, rod-like, and coil-like segments, and form gels with certain monomers and solvents like styrene or 2-ethylhexyl …
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A Discrete Monolayer Cardiac Tissue Model for Tissue Preparation Specific Modeling
… monolayers created by using microabrasion and micropatterning methods have provided a simplified in vitro system to study the effects of anisotropy and fiber direction on electrical propagation. Interpreting the behavior in these culture systems has often been performed using classical computer …
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Novel quantum dot and nano-entity photonic structures
… soft lithography method was also used for micropatterning and fabrication of photonic structures using QD/polymer and QD/titania films in conjunction to common UV and thermally curable materials. A solvent vapor smoothing process was found to significantly enhance the quality of the …
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The Roles of Focal Adhesion Assembly in Membrane Localization of Ion Channels and Action Potential Shape
… IK1 in the cell membrane. </p><p>Next we applied micropatterning of ECM proteins, pharmacological, and environmental manipulations to alter FA size and distribution in individual cells. Combining these techniques with single cell electrophysiology, confocal and total internal reflection …
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The mechanical regulation of Eph/ephrin signalling in the developing Xenopus brain
… assay combining stiffness gradient hydrogels and micropatterning. Since Eph/ephrin signalling in Xenopus RGCs is affected by substrate stiffness in vitro, and a stiffness gradient develops across the optic tectum at the time of innervation, my results suggest that mechanical cues could be …
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