Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"Micronucleus assay"”.
-
Direct and indirect effects of alpha-particle irradiations of human prostate tumor cells
… DU-145 cells were studied using the clonogenic assay, the comet assay and the micronucleus assay. A novel co-culture system was then designed to study the bystander effect transmitted through the cell culture medium. The micronucleus assay was used as endpoint for the bystander studies. A …
-
In vitro chemically-induced DNA damage in cancer patients and healthy individuals. The effect of genotoxic compounds in cells from polyposis coli, colon cancer patients and healthy individuals.
… habits were taken into consideration. The assays used in this study included the Comet assay, the Micronucleus assay, the Micronucleus ¿ FISH assay and the sister chromatid exchange assay. The food mutagens, PhIP and IQ, as well as titanium dioxide nanoparticles (TiO2 NPs) induced a dose …
-
The analysis of radiation-induced micronuclei in peripheral blood lymphocytes for purpose of biological dosimetry
… the suitability of the cytokinesis blocked (CB) micronucleus assay as a biodosimetric method using lymphocytes irradiated in vivo. The approach adopted to achieve this was to estimate whole body doses by relating micronuclei yields in patients undergoing radiotherapy treatment with an in vitro …
-
An Evaluation of the Occurrence of Micronuclei and other Nuclear Abnormalities in Fishes from the Great Lakes Basin, United States
… as a possible criterion for delisting. The micronucleus assay identifies genotoxic contamination by observing the presence of a micronucleus (MN), along with a primary nucleus within the cellular body. Nuclear abnormalities (NA) such as notching, lobes, blebbing, and binucleation are …
-
Micronuclei induction in AG01522 cells is independent of temperature and linear energy transfer
… dependence on the bystander effect. Using the micronucleus assay and primary human AG01522 fibroblast cells co-cultured as both the target and bystander cells, the incidence of micronuclei in both X-ray irradiated and alpha particle irradiated bystander experiments were ~2 fold over control …
-
Bystander Effects Due To Neutrons, And Low-Dose Hyper-Radiosensitivity To Gamma Rays In Human Cells Using Cytogenetics
… events were evaluated via the cytokinesis-block micronucleus assay in two normal human lymphoblastoid cell lines exposed to neutrons or gamma radiation. Micronuclei, nucleoplasmic bridges and nuclear buds were enumerated by recording the coincident presence of these endpoints within individual …
-
Changes in buccal cytome biomarkers in relation to ageing and Alzheimer’s Disease.
… ageing syndromes such as AD and DS. A buccal micronucleus cytome assay was developed and used to investigate biomarkers for DNA damage, cell proliferation and cell death in healthy young, healthy old and young Down’s syndrome cohorts. Cells with micronuclei, karyorrhectic cells, condensed …
-
Genotoxic effects of NSAIDs and hydrocortisone in bulk and nano forms in lymphocytes from patients with haematological cancers
… lymphocytes of healthy donors using the comet assay and the micronucleus assay. In order to determine any anti-cancer effects, these agents were also tested in peripheral blood lymphocytes in patients with haematological cancers. The glucocorticoid hydrocortisone was also evaluated for …
-
Genotoxic effects in human peripheral lymphocytes from healthy individuals and head and neck cancer patients after treatment with hydrogen peroxide and pembrolizumab liposome
… the healthy individuals by using the Comet and micronucleus assays. Western blotting and real-time polymerase chain reaction were performed to assess the potential of improving the repair mechanisms after treatment with naked Pembrolizumab and Pembrolizumab liposome. According to the results, …
-
Radiation-induced bystander fibroblasts both reduce and amplify micronuclei induction through the reciprocal bystander effect and the secondary bystander effect
… three types of secondary bystander cells. The micronucleus assay was used to analyze the amount of chromosome aberrations present. In the unirradiated secondary bystander population, an increase in percentage of binucleated cells with micronuclei from the background level to approximately the …
-
ASSESSING THE GENOTOXICITY OF TRICLOSAN IN TADPOLES OF THE AMERICAN BULLFROG, LITHOBATES CATESBEIANUS.
… for its simplicity and economy, is the micronucleus assay. A study was conducted assessing the genotoxicity of the widely used antimicrobial agent Triclosan to American Bullfrog tadpoles. Lithobates catesbeianus tadpoles were reared in glass aquaria containing ultra-high purity water and …
-
Genoprotective effect of aspirin and ibuprofen in human lymphocyte cells. Effect of nano and bulk forms of aspirin and ibuprofen on lymphocytes from breast cancer patients compared with those from healthy females
… healthy females as a control using the Comet and micronucleus assays. Western blot analysis (WBA) was used to investigate the effect of these drugs on XRCC3 and p53 proteins, whereas QPCR was to evaluate this effect on p53, cox1 and cox2 genes. Two hundred fifty ng/ml of ibuprofen (NP and bulk) …
-
Genoprotective effect of aspirin and ibuprofen in human lymphocyte cells. Effect of nano and bulk forms of aspirin and ibuprofen on lymphocytes from breast cancer patients compared with those from healthy females
… healthy females as a control using the Comet and micronucleus assays. Western blot analysis (WBA) was used to investigate the effect of these drugs on XRCC3 and p53 proteins, whereas QPCR was to evaluate this effect on p53, cox1 and cox2 genes. Two hundred fifty ng/ml of ibuprofen (NP and bulk) …
-
Molecular mechanisms of myricetin bulk and nano forms mediating genoprotective and genotoxic effects in lymphocytes from pre-cancerous and myeloma patients
… species- induced DNA damage using the Comet assay, micronucleus assay, cellular reactive oxygen species and glutathione detection assay, Western blotting, real-time polymerase chain reaction and immunofluorescence. Lymphocytes from the patient groups showed significantly higher levels of …
-
Molecular mechanisms of myricetin bulk and nano forms mediating genoprotective and genotoxic effects in lymphocytes from pre-cancerous and myeloma patients
… species- induced DNA damage using the Comet assay, micronucleus assay, cellular reactive oxygen species and glutathione detection assay, Western blotting, real-time polymerase chain reaction and immunofluorescence. Lymphocytes from the patient groups showed significantly higher levels of …
-
Cytogenetic Evaluation Of Low Dose Hyper-Radiosensitivity In Human Lymphoblastoid Cell Lines Exposed To Cobalt-60 Gamma Radiation.
… Cobalt-60 gamma radiation. The cytokinesis block micronucleus assay was used to determine the frequencies of micronuclei and nucleoplasmic bridges. To characterize the dependence of the cytogenetic damage on dose, univariate and multivariate regression analyses were used to compare the responses …
-
Effect of nanoparticles on human cells from healthy individuals and patients with respiratory diseases.
… obtained with human lymphocytes using the Comet assay to measure DNA damage. The effect of nanoparticles (40-70nm range) was studied in human sperm and lymphocytes in the dark, after pre-irradiation with UV and simultaneous irradiation with UV. The studies do provide some evidence that there are …
-
Cytogenetic Analysis Of Depleted Uranium Exposure And Age-Dependent Susceptibility To Ionizing Radiation
… blood lymphocytes with the cytokinesis blocked micronucleus assay (CBMN) and fluorescence in situ hybridization (FISH) whole chromosome painting. Study subjects are Gulf War I Veterans exposed to DU during friendly fire incidents in 1991 involving DU munitions which resulted in inhalation and …
-
Analysis of radiosensitivity in South African cervical and breast cancer patients
… increased chromosomal radiosensitivity. The G0-micronucleus assay (MN assay) and the γ-H2AX assay are two assays used in radiobiology to study DNA DSB and repair. Breast cancer is the leading cancer amongst South African women, with a lifetime risk of 1 in 34. Since most cancer patients in South …
-
Vergleich von in-vitro-Ergebnissen im Mikrokerntest und den klinischen Beobachtungen nach Bestrahlung
Hintergrund: Mikrokerne sind Chromosomenfragmente, die nicht in den Hauptkern integriert wurden und im Zytoplasma von proliferierenden Zellen nach ionisierender Strahlung oder Behandlung mit mutagensierenden Substanzen zu finden sind. In vielen Fällen konnte gezeigt werden, dass die …
Page 1 of 2