Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Micronuclei"”.
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FROM MICRONUCLEI TO EMT: MOLECULAR MECHANISMS OF GENOMIC INSTABILITY AND CELLULAR PLASTICITY
… this study reveals how p62 recognizes micronuclei through ubiquitination and proximity to mitochondria, with ROS-induced homo-oligomerization of p62 driving micronuclear envelope rupture. These findings uncover a critical mechanism by which p62 modulates nuclear membrane stability, …
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Micronuclei induction in AG01522 cells is independent of temperature and linear energy transfer
… the target and bystander cells, the incidence of micronuclei in both X-ray irradiated and alpha particle irradiated bystander experiments were ~2 fold over control averages. In the temperature experiment, there were no significant differences between bystander cells co-cultured with cold (4°C) …
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The analysis of radiation-induced micronuclei in peripheral blood lymphocytes for purpose of biological dosimetry
… was to estimate whole body doses by relating micronuclei yields in patients undergoing radiotherapy treatment with an in vitro radiation dose-response curve. These biologically derived estimates were then compared with the corresponding doses obtained by physical measurement and calculation. …
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An Evaluation of the Occurrence of Micronuclei and other Nuclear Abnormalities in Fishes from the Great Lakes Basin, United States
… 1 non-AOC site during the spring and fall, 2011. Micronuclei and/or NA were observed at all sites. Micronuclei were observed at differing occurrence rates among species. Brown bullhead (Ameiurus nebulosus ) expressed MN at a lower rate when compared to either largemouth bass (Micropterus …
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Regulation of DNA (Cytosine-5) Methyltransferase 1 in the cell cycle and its role(s) in doxorubicin-mediated micronuclei formation
… of extranuclear bodies during mitosis termed micronuclei, but the underlying causes remain unknown. Interestingly, my work provides strong evidence for the involvement of DNMT1 in promoting genomic instability in the form of micronuclei formation in human cancer cells upon sub-lethal exposure …
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Radiation-induced bystander fibroblasts both reduce and amplify micronuclei induction through the reciprocal bystander effect and the secondary bystander effect
… increase in percentage of binucleated cells with micronuclei from the background level to approximately the level of the primary bystander cells was observed, indicating that bystander cells can send damaging signals. The data also showed that there was a lower frequency of micronuclei formation …
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THE AUTOPHAGIC RECEPTOR P62 MODULATES MICRONUCLEUS STABILITY
Micronuclei are extranuclear bodies originated because of chromosome mis-segregation. In the past years, several studies identified the processes that are dysfunctional in micronuclei and described the structures that are disrupted within them, revealing how micronuclei differ from primary nuclei. …
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Age-related genetic and epigenetic chromosomal changes: A twin study
… chromosomal contents of spontaneously occurring micronuclei in identical and fraternal twins. A total of 115 individuals (48 twin pairs and 19 singletons) were evaluated, ranging in age from 7 to 85 years. As expected, micronuclei frequencies, which are indicative of genomic damage, significantly …
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Bystander Effects Due To Neutrons, And Low-Dose Hyper-Radiosensitivity To Gamma Rays In Human Cells Using Cytogenetics
<p>Chapter 2:</p> <p>Micronuclei have been used extensively in studies as an easily-evaluated indicator of DNA damage but little is known about their association with other types of damage such as nucleoplasmic bridges and nuclear buds. Radiation-induced clastogenic events were evaluated via the …
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Do heavy ions induce the bystander effect? : study to determine the induction of the bystander effect from Fe ion beam compared to X-rays in human keratinocytes
… radiation express biological responses such as micronuclei formation and genomic instability. Most published studies of the bystander effect have been conducted using alpha particles, a high LET radiation. A few studies have been done with low LET radiation. This project studies the bystander …
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Mechanical Confinement and Heat as Drivers of Aneuploidy in Cancer
… prometaphase progression, generates delayed micronuclei, and leads to heritable chromosome losses These effects phenocopy nocodazole, a microtubule inhibitor, and transcriptional analysis identifies confinement-induced dysregulation of mitotic programs. These results introduce the concept of …
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Changes in buccal cytome biomarkers in relation to ageing and Alzheimer’s Disease.
… and young Down’s syndrome cohorts. Cells with micronuclei, karyorrhectic cells, condensed chromatin cells and basal cells increased significantly with normal ageing (P<0.0001). Cells with micronuclei and binucleated cells increased (P<0.0001) and condensed chromatin, karyorrhectic, karyolytic …
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Disruption of Dna Polymerase Zeta Promotes An Innate Immune Response
… stress, as demonstrated by elevated levels of micronuclei formation. As the cellular consequences of this stress remain unexplored, genome-wide transcriptional changes were measured in <em>Rev3l </em>KO MEFs. The work in this thesis demonstrates that loss of pol ζ promotes activation of an …
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Mitochondrial dynamics in the radiation response of cancer cells
… Microscopy was used to quantify apoptosis, micronuclei and mitotic features, while cell cycle dynamics were analysed using flow cytometry. Results: Notably, modulation of mitochondrial fission using Mdivi-1 significantly increased radiation response in A549 cancer cells. Mdivi-1 reduced …
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The Non-canonical Function and Regulation of TBK1 in the Cell Cycle
… leads to a significantly higher number of micronuclei – a hallmark for tumorigenesis that correlates with defects in mitosis and cytokinesis. Taken together our work has uncovered a novel function for the NAP1-TBK1 complex during mitosis and establishes that overactivation and …
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Cytogenetic Evaluation Of Low Dose Hyper-Radiosensitivity In Human Lymphoblastoid Cell Lines Exposed To Cobalt-60 Gamma Radiation.
… assay was used to determine the frequencies of micronuclei and nucleoplasmic bridges. To characterize the dependence of the cytogenetic damage on dose, univariate and multivariate regression analyses were used to compare the responses in the low- (HRS) and high-dose response regions. Our data …
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The effect of the CCI mutation on cell cycle morphogenesis in Paramecium Tetraurelia
… the 6-rowed oral primordium stage. At this stage micronuclei are in late anaphase. Oral morphogenesis is arrested up to this stage in mutant cells at the restrictive temperature, as are later stages of micronuclear division. These results are interesting because they suggest that micronuclear DNA …
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Investigating The Role of Abro1 In Dna Damage-Induced Immune Response
… cGAS binding to cytoplasmic DNA or localizing to micronuclei in response to DNA damage. Here, I found that replication stress induced by Abro1 deficiency leads to ssDNA accumulation in the cytoplasm triggering activation of the cGAS/STING pathway with upregulation of inflammatory genes and …
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The functional and molecular characterisation of the novel DNA damage repair protein Bod1L
… to damaged DNA. Bod1L depletion leads to micronuclei formation in undamaged cells and hypersensitivity to genotoxic stress. Bod1L binds to chromatin and is phosphorylated by ATM at sites of DNA damage. In Bod1L-depleted cells, ATM activity is normal, but there is over-accumulation of MDC1 …
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Genotoxic effects of oestrogens and nano-NSAIDs: Genotoxic effects of oestrogens in vivo and nano- and bulk forms of NSAIDs on blood samples from prostate cancer patients
… of strand breakage and lowered the numbers of micronuclei generated compared with their bulk forms. There was no clear difference between the sensitivity of the healthy controls and the prostate cancer patients, with only one individual showing evidence of heightened sensitivity.
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