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Showing 1 to 20 of 63 for “"Microinjection"”.
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Microinjection moulding and material testing of polyolefins on a small scale
De onderzoeksgroep SMaRT (KU Leuven) concentreert zich op polymeren en colloïdale dispersies om nieuwe materialen te ontwikkelen en hun verwerkingsgedrag te bestuderen. De ontwikkeling en studie van nieuwe materialen is een uitzonderlijk lang en duur proces. Voor de studie van het verwerkingsgedrag …
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Universal robot for automated microinjection with applications in transgenesis and cryopreservation
Microinjection is the process of injecting a small amount of solution into biological organisms at a microscopic level using a glass micropipette. It is a widely utilized technique with a wide range of applications in both fundamental research and clinical settings. However, microinjection is an …
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Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection
… We used autologous platelet mitochondrial microinjection to improve the mitochondrial quality and quantity of aged oocytes to improve their pregnancy potential. Metaphase II oocytes were collected from super-ovulated female hamsters and prepared for biochemical and morphological analysis. …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… vitro. Experiment 1 examined the effect of DNA microinjection at 11, 15 and 19 h after fertilization (haf) on survival rate and DNA detection frequency by polymerase chain reaction (PCR). There was no difference in transgene detection frequency between treatments (53% at 11; 50% at 15; 48% at 19 …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… technique (PCR), and evaluated the use of DNA microinjection, in vitro culture, morula bisection, and PCR for production and selection of transgenic bovine preimplantation embryos. In vivo fertilized one-cell bovine embryos were cultured in TCM-199 (n=46), co-cultured with bovine oviductal …
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Microfuidic Devices and Open Access Tool for Localized Microinjection and Heart Monitoring of Drosophila Melanogaster
… reversible immobilization, and localized microinjection of intact Drosophila larvae for recording heart activities upon injection of controlled dosages of different chemicals. In objective 2. we have developed software for in-vivo quantification of essential heartbeat parameters on intact …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
… embryos and also supply a source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly (2X) oocyte recovery frequencies were compared. No differences in recovery per session were observed (IX = 6.8 vs. 2X = 6.3 oocytes/session; p>O.1 0). However, the 2X group generated more …
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Assessment of Murine Embryo Development Following Electroporation and Microinjection of a Green Fluorescent Protein DNA Construct
… integration following pronuclear or cytoplasmic microinjection of condensed or linear green fluorescent protein DNA into murine embryos using electroporation. In experiment 1, the effect of embryo orientation (group or linear) within the electroporation chamber on development was evaluated using …
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In-vitro developmental potential of bovine oocytes obtained by transvaginal follicular aspiration as related to their morphological quality and after microinjection of DNA
The development of oocytes of differing quality retrieved using transvaginal follicular aspiration (TVFA) and following DNA injection was examined. Eight cows were subjected to twice weekly TVF A for 16 wk. Oocytes retrieved were graded and placed in an in-vitro maturation, fertilization and …
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Control of endometrial secretion in cattle and production of transgenic swine
… schemes. Pronuclear microinjection and zygote culture in excised mouse oviducts also was assessed. Synchronization/superovulation procedures were: 1) sows observed for estrous behavior (NAT), 2) cyclic gilts synchronized with altrenogest (ALT) for 15 to 19 d and …
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Evaluation of techniques for the production of transgenic animals
… to detect transgene presence after pronuclear microinjection of mouse zygotes cultured to various stages of development. The transgene was detected in 88% of 1-cell, 88% of 2-cell, 44% of 4- cell, 40% of morula, and 29% of blastocysts. By comparison, the integration frequency for transgenic …
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A Study of Heat Transfer at the Cavity-Polymer Interface in Microinjection Moulding. The effects of processing conditions, cavity surface roughness and polymer physical properties on the heat transfer coefficient
… the cooling behaviour of polymers during the microinjection moulding process. The work included bespoke experimental mould design and manufacturing, material characterisation, infra-red temperature measurements, cooling analysis and cooling prediction using commercial simulation software. To …
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Microsurgical Fertilization of Mammalian Eggs: An Assessment of Clinical Utilization
… of the human egg's ability to survive the sperm microinjection procedure and participate in the early events of fertilization. This study found no evidence of ultrastructural damage to either hamster or human eggs surviving the microinjection procedure although abnormal fertilization events were …
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Cocaine- and Amphetamine-Regulated Transcript Peptide Attenuates Baroreflex in the Rat.
… 3 nmol) by intracisternal or bilateral intra-NTS microinjection consistently attenuated the phenylephrineinduced bradycardia. In contrast, CARTp antibody potentiated the bradycardia produced from phenylephrine. Microinjection of saline, normal rabbit serum, or concomitant injection of CARTp and …
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Anatomical and Physiological Characterization of Striatal Activation of the Cerebral Cortex
… were used as follows: First, intracranial microinjection was used to deliver drugs directly into the striatum and motor cortex. A guide cannula was implanted into the right striatum/motor cortex of each TH KO mouse, and a single microinjection of L-dopa/ D1 agonist SKF81297/ D2 agonist …
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Identification and Characterization of a Relish-type NF-kB, DvRelish in Dermacentor variabilis, the American Dog Tick
… Tick infection assays were performed using microinjection and capillary feeding technique methodologies to identify dvrelish expression in response to SFG Rickettsia infection. Microinjection of 107 R. rickettsii induced the increased expression of dvrelish in hemocytes at 1 hour post …
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Molecular Substrates And Neuronal Network Subserving Audiogenic Kindling In Genetically Epilepsy-Prone Rats
… GEPR-9s. We performed a one-time focal bilateral microinjection of an AC activator, MPB forskolin {7-Deacetyl-7-[O-(N-methylpiperazino)-γ-butyryl] - forskolin dihydrochloride} (25-100 pmol/side) into the LA of non-kindled GEPR-9s. The one-time microinjection of MPB forskolin in GEPR-9s, across all …
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Examining the Roles of GABAA Receptor Subtypes in Anxiety and Anxiolysis: Focusing on the Basolateral Amygdala
… <em>selective PAMs</em> were administered via microinjection into the BLA; however, these local injections did not significantly affect motor activity at the doses tested. In the experiment examining systemic injections of the non-selective BZ chlordiazepoxide (CDP), we found that CDP induced …
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Factors affecting the efficiency of gene transfer in mice
… to optimize the overall efficiency of pronuclear microinjection, we designed experiments to: 1) test the best developmental stage for transferring injected embryos to obtain pregnancies and transgenic pups; 2) determine the optimum number of injected embryos transferred to obtain pregnancies and …
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The Mitotic Spindle Mediates Inheritance of the Golgi Ribbon Structures
… Golgi stacks are polarized and transport cargo. Microinjection of Golgi extract together with tubulin or incorporation of spindle materials rescues Golgi ribbon formation. Therefore, the factors required for postmitotic Golgi ribbon assembly are transferred by the spindle, but the constituents of …
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