Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Microfluidic Cell"”.
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Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
… are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a …
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Microfluidic cell culture chambers with nanoporous walls for chemical communication
… microbes we propose a method to engineer a microfluidic co-culture platform, the Microfluidic Cell Culture Chambers, which mimics natural conditions for bacterial growth. The key innovation is to physically isolate bacteria while allowing chemical communication through metabolite diffusion. …
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Influence of Fibroblasts on Metastatic Cancer Cell Drug Resistance in a 3D Microfluidic Cell Array
… Our lab has previously developed a three layered microfluidic cell array (3D μFCA) to reconstruct the relevantly spatial configuration of tumor and microvasculature found <em>in vivo</em> in order to develop a more efficient tool of high throughput drug discovery and screening. In this study, we …
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Immunoassay sensitivity and kinetic enhancement in cell culture media using electrokinetic preconcentration
The microfluidic cell culture enables the study of cell signaling in previously impossible or impractical ways by allowing the precise spatial and temporal control of the microenvironment to better mimic in vivo conditions. Microfluidic techniques allow the creation of exact culture geometries, …
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Novel micro/nanofluidic system for separation and monitoring of cells and proteins in perfusion
… complex biopharmaceutical products based on cells and proteins are transforming modem medicine and advancing treatments for many health conditions. Continuous biomanufacturing is one of the top technology trends in the biopharmaceutical industry to improve biological product quality and …
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Microfluidic Electrical Impedance Spectroscopy
… of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created …
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Design, Fabrication, and Implementation of a Single-Cell Capture Chamber for a Microfluidic Impedance Sensor
A microfluidic device was created for single-cell capture and analysis using polydimethylsiloxane (PDMS) channels and a glass substrate to develop a microfluidic single-cell impedance sensor for cell diagnostics. The device was fabricated using photolithography to create a master mold which in turn …
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Development of Microfluidic 3D Cell Array with Tumor-Stroma interaction for Cancer Immunotherapy Drug Screening
… which is composed of a variety of different cell types, for immunotherapy validation is difficult, costly, and complicated. There is therefore an unmet need for novel approaches to replicate TME for pre-screening these drug candidates. Moreover, several studies have demonstrated that the …
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Convective intracellular macromolecule delivery for cell engineering applications
Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering, cell labeling, and other biomedical applications. Current standard methods of intracellular delivery, such as viral transduction and electroporation, do not meet the growing needs in the cell …
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Effects of interstitial flow on tumor cell migration
… transport of fluid through tissue extracellular matrix. This creeping fluid flow has been shown to affect the morphology and migration of cells such as fibroblasts, cancer cells, endothelial cells, and mesenchymal stem cells. However, due to limitations in experimental procedures and …
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New Sensing Modalities for Bacterial and Environmental Phenomena
Intercellular communication is a ubiquitous phenomenon across all domains of life, ranging from archaea to bacteria to eukarya. In bacteria, this is often achieved using small molecules that allow bacteria to sense and respond to environmental cues about the presence, identity, and number of …
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Microfluidic platforms to evaluate the effects of drugs on fertility and proteins on cancer progression – a focus on cisplatin, alpha-smooth muscle actin, and glial cell line-derived neurotrophic factor
… is necessary to get reliable information about cell responses and test drug efficacy and toxicity. Classical cell culture systems facilitate a wide range of clinical in vitro studies. These cell culture systems often consist of static and flat platforms that poorly mimic the conditions in living …
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Multiphase flow in porous media: the impact of capillarity and wettability from field-scale to pore-scale
… when one fluid displaces another in a patterned microfluidic cell. The pore-scale portion of this Thesis focuses on the impact of wettability on fluid-fluid displacement inside a capillary tube. We show that the contact line movement is strongly affected by wettability, even in regimes where …
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Microfluidic Dual-Gel Cell Culture Model For Studying Cell Migration Under Controlled Interstitial Flow
… occurring in the interstitial space of the extracellular matrix (ECM), affects the migration of many cell types through individual and synergistic contributions of several biophysical and biochemical phenomena. Notably, interstitial flow is linked to ECM permeability and provides direct …
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Technologies for the isolation of circulating tumor cells
Metastasis, the spread and growth of tumor cells from the primary site to distant organs, is arguably the most devastating and deadly attribute of cancer, and is ultimately responsible for 90% of cancer-related deaths. Circulating tumor cells (CTCs) are exceedingly rare cells found in the whole …
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Microfluidic single-cell technologies for assaying lymphocyte interactions
Immune cells do not live in isolation but interact to coordinate their many actions. One of the chief routes they foster communication is through direct physical interactions that enables them to read and interpret signals mediated at membrane interfaces. Despite the critical importance of these …
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Experimental Methods in Support of the Development of a Computational Model for Quorum Sensing in Vibrio fischeri
The quorum sensing signaling system based on intercellular exchange of N-acyl-homoserine lactones is used by many proteobacteria to regulate the transcription of essential genes in a signal density-dependent manner. It is involved in a number of processes including the development of highly …
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Modeling of the electrochemical conversion of CO2 in microfluidic reactors
… aqueous electrochemical reduction of CO2 in a microfluidic reactor, which has been demonstrated experimentally to be an effective reactor and a versatile analytical tool. This thesis focuses on developing a mathematical modeling framework for the electrochemical conversion of CO2 to CO in …
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Combining a microfluidic platform with LC-MS for the detection of induced hepatotoxicity using primary hepatic spheroids
… as time consuming and very costly. Simple sub-cellular models can provide high throughput data allowing early detection of many drugs doomed to fail, but do not encompass the complexity found in whole cell based models, which provide more in-depth data. Cell culture models have evolved from …
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