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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 358 for “"Microarrays"”.
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Genome-wide analyses using bead-based microarrays
Microarrays are now an established tool for biological research and have a wide range of applications. In this thesis I investigate the BeadArray microarray technology developed by Illumina. The design of this technology is unique and gives rise to many computational and statistical challenges. …
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Photoelectromechanical synthesis of low-cost DNA microarrays
… for target sequencing using DNA from custom microarrays have demonstrated that microarrays can effectively be used as the world's cheapest sources of complex oligonucleotide pools. Unfortunately, commercial custom microarrays are expensive and not easily accessible to academic researchers, …
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GeneSieve: A Probe Selection Strategy for cDNA Microarrays
… (ESTs). ESTs are widely used as the probes for microarrays. Designing custom microarrays, rich in genes relevant to the experimental objectives, requires selection of probes based on their sequence. We have designed a probe selection method, called GeneSieve, to select EST probes for custom …
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APPLICATIONS OF MICROARRAYS AND STEM CELLS FOR REGENERATIVE MEDICINE
Stem cells are viewed as a potential resource for cell-based therapy and regenerative medicine. Understanding the genes that govern the special properties of stem cells has implications for both stem cell biology and regenerative medicine. This information will ensure the quality of the cells, the …
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Statistical Design And Imaging Of Position-Encoded 3D Microarrays
We propose a three-dimensional microarray device with microspheres having controllable positions for error-free target identification. Here targets: such as mRNAs, proteins, antibodies, and cells) are captured by the microspheres on one side, and are tagged by nanospheres embedded with …
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Analyse des Hitzeschocks bei Neisseria meningitidis mit DNA-Microarrays
Das Gram negative Bakterium Neisseria meningitidis ist weltweit ein bedeutender Erreger der bakteriellen Meningitis. Obwohl das ausschließlich humanpathogene Bakterium in bis zu 25% der Europäischen Bevölkerung die oberen Atemwege als harmloser Kommensale besiedelt, kommt es unter bestimmten, noch …
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Algorithms for improving the design and production of oligonucleotide microarrays
Microarrays are a ubiquitous tool in molecular biology with a wide range of applications on a whole-genome scale including high-throughput gene expression analysis, genotyping, and resequencing. Although several different microarray platforms exist, we focus on high-density oligonucleotide arrays, …
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Typing and genome comparison of Neisseria meningitidis by DNA-microarrays
… have been followed. The first one evaluated microarrays based on polymorphism-directed oligonucleotide design for typing of N. meningitidis adopting the multilocus sequence typing (MLST) concept. The number of oligonucleotides needed to cover all known polymorphisms was much lower compared to …
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Use of peptide microarrays for mapping viral b cell epitopes
… manufacturing multipurpose high-density chemical microarrays." Qsar & Combinatorial Science 25(11): 1069-1080. Frank, R. (1992). "Spot-Synthesis - an Easy Technique for the Positionally Addressable, Parallel Chemical Synthesis on a Membrane Support." Tetrahedron 48(42): 9217-9232.
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Study of cell-cell communication using 3D living cell microarrays
Cellular behavior is not dictated solely from within; it is also guided by a myriad of external cues. If cells are removed from their natural environment, apart from the microenvironment and social context they are accustomed to, it is difficult to study their behavior in any meaningful way. To …
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Analyse des Transkriptoms von Neisseria meningitidis Serogruppe B mit DNA-Microarrays
Die Anwendbarkeit eines Oligonukleotid-basierten Microarray-Systems zur Transkriptomanalyse von Neisserie meningitidis Serogruppe B wurde im Vergleich mit einem cDNA-basierten System demonstriert. Hierzu wurde für ein Subset von 60 Genen die Eigenschaften anhand von Parallelhybridisierungen sowie …
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Utilization of ferrioxamine microarrays for the rapid detection of pathogenic bacteria
… a proteomic BLAST search, and screened against microarrays patterned with FOx conjugates for detection using label-free optical imaging. Aspects such as inkjet printing and surface chemistry, iron-limiting conditions and bacterial selection protocols, and linker conjugate design were addressed …
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Fabrication of DNA microarrays on Poly (methylmethacrylate) substrates for biomolecular reporting
DNA microarrays require the use of substrates with well-established surface modification / probe attachment chemistries. Glass/quartz have been widely adopted as typical support materials since their surface modification chemistries which involve the use of siloxane –based chemistries have been …
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3D-DNA-Chips : oberflächengebundene funktionelle Polymernetzwerke als Matrix für Nukleinsäure-Microarrays
… DNA-Chips konventionellen medium-density DNA-Microarrays hinsichtlich der Flexibilität der Anwendungen und der Eigenschaften überlegen sind. Um die Signalintensität zu steigern, müssen die Sondenmoleküle in einem quellfähigen dreidimensionalen Netzwerk immobilisiert sein. Es konnte gezeigt …
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Development of protein microarrays for the investigation of host-pathogen interactions
Varicella-Zoster virus (VZV) is a human herpesvirus (causing chickenpox and shingles upon reactivation) whose diagnosis is particularly important in pregnancy and immunocompromised patients. Current sérodiagnostic assays are either based on crude whole cell lysates or glycoprotein preparations …
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Discovery of Novel Ubiquitin- and Methylation-Dependent Interactions Using Protein Domain Microarrays
… this dissertation, I applied two protein domain microarrays identifying novel readers for histone H2Aub1 and H2Bub1, and H3TM K4me3. Ubiquitinations of histone H2A at K119 (H2Aub1) and histone H2B at K120 (H2Bub1) function in distinct transcription regulation and DNA damage repair pathways, …
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Entwicklung von Microarrays für die Multiparameteranalytik und Etablierung einer Multiplex-OnChip-PCR
… parallel in einem Versuchsansatz erfolgen kann. Microarrays stellen hingegen die benötigten Voraussetzungen zur Verfügung, um als Werkzeuge für die Multiparameteranalyse in verschiedensten Anwendungsbereichen zu dienen. Ein Schwerpunkt dieser Arbeit war es, Multiplex-PCRs und diagnostische …
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Studien der Genexpressionsänderung während der Entwicklung von Drosophila melanogaster mittels DNA-Microarrays
… Diese wurden als Basis für die Herstellung des Microarrays verwendet. So war es möglich die Vorteile computergestützter in silico- Berechnungen der möglichen Gene mit experimentellen Analysen zu kombinieren, um die Expression von Genen zu detektieren. Durch Optimierung der Protokolle und …
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Gene expression profiling of patients with Polycythaemia Rubra Vera using cDNA microarrays
In this work, several attempts to elucidate the physiological role of the murine PRV-1 gene by whole mount in-situ hybridisation experiments did not yield reproducible results. Nevertheless, expression of the mPRV-1 mRNA could be demonstrated from embryonic day 6.5 on by RT-PCR experiments. In …
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