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Showing 1 to 20 of 31 for “"Michaelis-Menten kinetics"”.
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Site-directed mutagenesis of the ATP-binding site in spinach rubisco activase
… the cDNAs. A significant difference in the kinetics of the ATP response between the two cloned isoforms was observed. The 45 kD polypeptide exhibited sigmoidal kinetics for both rubisco activase and ATPase activities, while the activities of the 41 kD isoform appeared to follow standard …
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Content and Activity of Cytochrome P450 3A in Rat Brain Microsomes and Mitochondria
… mitochondria and microsomes to determine the Michaelis-Menten kinetics of the formation of CYP3A-mediated hydroxylated metabolites of midazolam by UPLC-MS/MS. We also aim to analyze the protein levels of CYP3A in the rat brain mitochondria and microsomes by Western blot analysis.</p> …
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Effector Response of the Aspartate Transcarbamoylase From Wild Type Pseudomonas Putida and a Mutant with 11 Amino Acids Deleted at the N-terminus of PyrB.
… Velocity-substrate plots without effectors gave Michaelis-Menten kinetics in all cases. Deleting 11 amino acids did not affect dodecameric assembly but altered effector responses. When carbamoylphosphate was varied, the mutant enzyme was inhibited by UTP while the wild type enzyme was activated …
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Development and Microscaling of a Novel Glucose Biosensor for Application in a Minimally Invasive Sampling Platform
… oxidase on a gold electrode, and which obeys Michaelis-Menten kinetics and were characterized using their Imax and Km values. The sensor includes this transducer, a micropotentiostat, a power supply, and an ADC that communicates with the e-Mosquito microprocessor. Testing of the transducer …
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Kinetics of Acid Inactivation of Soybean Lipoxygenase and Its Effects on the Functional Properties of Soy Protein
… at pH 3.0 and below. From a study of enzyme kinetics of lipoxygenase extracted from the acidified soy slurries, significant changes were observed with the values of the Michaelis constant and in the enzyme inhibition patterns when the enzyme was exposed to pH of about 4.6 and below. This …
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IN VITRO AND IN VIVO KINETIC MODELING OF DIAZEPAM METABOLISM
… it is crucial to understand the mechanism and kinetics of drug metabolism by various drug-metabolizing enzymes (DMEs). Cytochrome P450 enzymes (CYPs) are responsible for the metabolism of more than 60% of the top 200 prescribed drugs. X-ray and NMR data of CYP enzyme suggest that relatively …
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Biochemical characterization of Aspergillus fumigatus SidA: a flavin-dependent N-hydroxylating enzyme
… FAD cofactor. The enzyme demonstrated typical Michaelis-Menten kinetics in a product formation assay with respect to L-ornithine, but similar experiments as a function NADH and NADPH indicated inhibition at high coenzyme concentrations. Af SidA is highly specific for its substrate; however, it …
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Enzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target
… other inhibitors in the group. We determined the Michaelis-Menten kinetics for five compounds representing each of the classes. Examination of compounds belonging to Class I indicates that these compounds do not follow normal Michaelis-Menten kinetics. Instead, these compounds inhibit N5-CAIR …
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Understanding PilB, The Type IV Pilus (T4P) Assembly ATPase
… This research focuses on understanding the kinetics of PilB, the T4P assembly ATPase. PilB crystal structure exhibits an elongated hexamer with 2-fold symmetry indicating a symmetric rotary mechanism model. Except for its structure, the symmetric rotary mechanism of PilB has not been …
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Modelling Denitrification In Soil
… in the denitrification process is modelled using Michaelis-Menten kinetics. We begin this thesis by reviewing existing work, discussing some of its limitations and proposing various alterations. Later, we present a preliminary model of the oxygen distribution within a soil with the aim of …
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Metabolic Pathway Optimization with Data Driven Approaches
… datasets generated using fully reversible Michaelis-Menten kinetics. All parameters are randomly sampled from a maximum entropy distribution assuming no prior knowledge on the system. For a pathway up to 15 nodes, the results show over 90% accuracy in predicting step with the largest …
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MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… measurable intrinsic GTPase activity that obeyed Michaelis-Menten kinetics. The KM, kcat and catalytic efficiency of the enzyme were calculated to be 0.820 mM, 0.175 sec-1 and 213 M-1sec-1 respectively. Compared to other GTPases, the KM was higher, indicating a low affinity of the protein for GTP. …
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Understanding the Mechanism of Succinyl-CoA Synthetase Catalysis from Structural Studies
… roles of human ATPSCS and GTPSCS, their kinetics and structures were compared. Human ATPSCS was co-crystallized with ADP or GDP, and the structures were determined at 3.1 Å-resolution. Both enzymes displayed Michaelis-Menten kinetics for all substrates except succinate, for which human …
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Sodium and glucose transport across the in vitro perfused midgut of the blue crab, Callinectes sapidus Rathbun
… animal species. The flux displayed saturation kinetics and was ouabain-sensitive. The flux did not vary with environmental salinity, thus providing no evidence for an active regulatory role of the midgut in sodium balance. A reduction of passive permeability with salinity, however, was …
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Bacterial interactions and enzyme kinetics in environmental polyester degradation
… PBSeT enrichment experiments. Monoculture Monod kinetics on the monomers of the polymer revealed each bacterium’s preference and competitiveness for PBSeT substrates. Subsequently, coculture incubations with the whole plastic revealed how these dynamics manifest in complex systems. Informed by …
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Purification and Characterization of Native and Recombinant Dipeptidyl Aminopeptidase 1 of Plasmodium falciparum
… whereas papain-treated DPAP1 deviated from Michaelis-Menten kinetics. Using a combinational dipeptidyl substrate library, the substrate specificities of native and recombinant (trypsin-activated) DPAP1, as well as of human cathepsin C were profiled. We find that both DPAP1 and human …
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Characterisation of the sulfotransferases catalysing the sulfation of xenobiotics and steroids in bovine liver
… bovine livers. Male liver cytosol followed Michaelis-Menten kinetics whereas the female liver cytosol displayed partial substrate inhibition. This suggests that different enzymes have been involved in the biotransformation of 4-nitrophenol in the male and female liver. Activity towards …
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Cerium oxide Nanoparticles: Their Phosphatase Activity and its Control
… activity for all the substrates exhibited Michaelis-Menten kinetics. Although the phosphatase activity of these nanoparticles is slow (turnover rate) as compared to real biological phosphatases, it can be used as a model catalytic activity to follow other catalytic activities that are …
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Binding and transport of calcium by synthetic analogues of ionomycin
… was found to be a saturable process which obeyed Michaelis-Menten kinetics. It was dependent of the pH in the aqueous source phase and independent of the pH in the receiving phase. Both the carboxyl and the 13-diketone groups were ionized in the transport of calcium, indicating the stoichiometry …
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Production and Use of Alginate Beads Containing Yeast in a Novel Fluidised Bed Bioreactor
… the consumption of glucose within beads based on Michaelis-Menten kinetics and the diffusion of glucose into beads. Application to the experimental results showed maximum reaction velocity for the conversion of glucose to ethanol was independent of bead diameter and alginate concentration. It also …
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