Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Michael-type addition"”.
-
Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides
… of Ser/Thr residues and cyclization via Michael-type addition. The remarkably broad substrate scope of the synthetase ProcM inspired us to explore its mechanism in detail (chapter 2). My studies on ProcM revealed the directionality of dehydration, the order of cyclization, and that, …
-
Antibiotic-free antibacterial hydrogels for wound healing applications
… by Schotten-Baumann reaction and thiol-ene Michael type addition. Once again, the material was chemically and mechanically characterised, and biological studies were carried out: >99% reduction in the bacterial cell count was observed for both S. aureus and E. coli, with high …
-
Investigation of the Lacticin 481 Synthetase and Its Substrate LctA in the Production of the Antibiotic Lacticin 481
… followed by stereoselective intramolecular Michael-type addition of cysteines onto the unsaturated amino acids. Class I lantibiotics are biosynthesized by a LanB dehydratase and a LanC cyclase while class II lantibiotics are generated by one bifunctional LanM enzyme. Lacticin 481 synthetase …
-
Investigations into the roles and molecular mechanisms of LanC-like proteins
… for lanthipeptide synthesis and activity is a Michael-type addition of a cysteinyl thiol to dehydroalanine and dehydrobutyrine, catalyzed by the prokaryotic lantibiotic cyclase (LanC) or the cyclase domain in a multi-functional lantibiotic synthetase. Intriguingly, despite the lack of evidence …
-
i) Design and Synthesis of Butadiene Substrates for Organocatalytic Transformations ii) Synthesis of Functionalized Biaryls and Pyran Derivatives
… butadiene substrates for the study of vinylogous Michael-type addition chemistry through organocatalytic means. The synthesis of these electron-deficient butadienic systems proved to be quite challenging and required extensive reaction screening in order to access these substrates in synthetically …
-
Step-growth thiol-ene photopolymerization to form degradable, cytocompatible and multi-structural hydrogels
… hydrogels have not been fully characterized. In addition, existing thiol-ene photopolymerizations often used type I photoinitiators in conjunction with an ultraviolet (UV) light source to initiate gelation. The use of cleavage type initiators and UV light often raises biosafety concerns. The …
-
Surface Plasmon Resonance Imaging Biosensors for Cancer Diagnosis: Detection of Circulating Tumor DNA
… probes has been obtained by injecting PNA wild-type and PNA mutated solutions into a microfluidic system coupled to SPR sensor chip. The extremely low concentration of genomic DNA required an improvement of SPRI detection capabilities, by using functionalized gold nanoparticles to amplify the …
-
Adiciones 1,4-conjugadas a deshidroalaninas quirales para la síntesis de aminoácidos no naturales
… of La Rioja). α,β-Dehydroamino acids are a type of electrophiles widely employed in 1,4-conjugate additions with nucleophiles of different nature to obtain unnatural amino acids. However, their chiral version that would allow access to such enantiomerically pure amino acids is still …
-
Exploring and understanding lantibiotic biosynthesis
… structural and catalytic protein molecules. In addition to its role in protein synthesis, the ribosome also produces small non-catalytic peptides that exert antimicrobial properties. The recent surge in the availability of genome sequences from a wide variety of bacterial sources has led to the …
-
Development and characterisation of a heparinised fibrin hydrogel as a delivery vehicle for regenerative medicine
… for retention of thrombin-based polymerisation. Additionally, the potential of co-modification with polyethylene glycol (PEG) enhancing heparinisation and improving fibrin resistance to proteolytic degradation was assessed. The modified forms of fibrin hydrogel were then assessed for their …