Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 7 of 7 for “"MiSeq Illumina"”.
-
Effect of Crude Oil on Light Attenuation and the Resulting Impacts on Marine Microbial Phototrophs and Heterotrophs
… 16S rRNA and psbA genes was also performed. Miseq Illumina sequencing analysis was performed to measure the abundance of bacteria and phototrophs. A qPCR was also performed to quantify phototrophs and HCB and UPLC analysis was done to quantify phototroph`s pigments. With continuous light, …
-
Characterizing the Microbiome of Scottish Freshwater Pearl Mussels (Margaritifera margaritifera): A Metabarcoding Analysis using Next-Generation Sequencing
… Technology) for full-length amplicons and MiSeq (Illumina) for short amplicons, enabling a comparative analysis of platform performance at the species level. Results revealed that the mussel microbiome was distinct from that of river water, with significant differences even at high …
-
Analysis of the microbiota of human milk and baby faeces in Nigeria, using molecular and culture-based approaches
… ribonucleic acid (16S rDNA) NGS sequencing (MiSeq Illumina). Human milk has a highly personalised microbiota with a lot of inter-individual variabilities, according to the present study. It was revealed that the milk microbiota of mothers from Nigeria is characterised by the high dominance of …
-
Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… for Next Generation Sequencing (NGS) with MiSeq Illumina V2 systems. The sequence reads generated were assessed for quality, pre-processed, and assigned to taxa using two bioinformatics pipelines. The first followed the method for analysing metabarcoding dualindex data described by Sickel …
-
Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… for Next Generation Sequencing (NGS) with MiSeq Illumina V2 systems. The sequence reads generated were assessed for quality, pre-processed, and assigned to taxa using two bioinformatics pipelines. The first followed the method for analysing metabarcoding dual- index data described by Sickel …
-
Change in the Structure of Soil Microbial Communities in Response to Waste Amendments
Soil microbial communities are affected extensively by addition of amendments to their environment. Of particular concern is the addition of poultry litter, which contains a substantial C, energy, and nutrient supply, but also antibiotic resistance genes (ARG), antimicrobials, and a multitude of …
-
A Study of the Genetics of Root Nodulation in Pigeonpea (Cajanus cajan) Using Indigenous Rhizobia
… were prepared using the Nextera protocol (Illumina, USA) and paired-end (300bp x 2) sequenced on a MiSeq (Illumina) sequencer at the Biotechnology Platform, Agricultural Research Council-Onderstepoort Campus (Pretoria, South Africa). The genome was a large circular chromosome (6,297,373 bp) …