Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 335 for “"Methyltransferase"”.
-
Studies on the Histone Methyltransferase G9a
… conditional inactivation of the H3K9-specific methyltransferases G9a and GLP. These studies demonstrate that G9a is essential for B cell development in the mouse, but is dispensable for T cell development. The defect in B lymphopoiesis in the absence of G9a is caused by a block in development …
-
Structure and Biochemistry of RsrI Methyltransferase
Comparison of the dimer interface of M.RsrI with the recent M.MboII structure and sequence alignments allowed identification of a conserved dimerization motif. I disrupted the dimerization of M.RsrI by adding N-acetyl-phenylalanine as a competitive inhibitor and by mutation of a serine located in …
-
Chemotaxis Methyltransferase Ii From Bacillus Subtilis
… We have purified and characterized a chemotaxis methyltransferase (methyltransferase II) from Bacillus subtilis. By using a combination of DEAE Bio-Gel A chromatography, CM Bio-Gel A chromatography, ammonium sulfate fractionation, and S-adenosylhomocysteine affinity chromatography, a virtually …
-
Novel antibiotics from DNA adenine methyltransferase inhibitors
… virulence on the activity of Dam, an adenine methyltransferase, makes it an attractive target for novel antibiotics. Dam from Yersinia pestis, the plague causing bacteria, was expressed and purified by nickel affinity chromatography. A plasmid containing the methylation sensitive restriction …
-
Structural and mechanistic studies of class III lanthipeptide biosynthesis, an α-N methyltransferase, and a sabath yerba mate methyltransferase
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
-
The Role of Potassium in Betaine-Homocysteine Methyltransferase
… methionine synthase and betaine-homocysteine methyltransferase (BHMT). Methionine synthase function depends on adequate availability of vitamin B12 and folate which links cardiovascular disease risk to nutritional status. The Hcy remethylation pathway through BHMT, however, requires only the …
-
Structures and Regulation of the N6-Methyladenosine Methyltransferase METTL16
… and decay. The m6A modification is deposited by methyltransferases using S-adenosylmethionine (SAM) as the methyl donor. Determining the specificity and mechanism of action of m6A methyltransfersaes is an essential step in dissecting the role of the m6A modification in RNA metabolism. Two enzymes …
-
Role of Betaine-Homocysteine S-Methyltransferase in Homocysteine Methylation
In mouse liver homogenates, 1000 mumol/L D,L-CBHcy did not inhibit methionine synthase, CBS and cystathionase activities but 5 mumol/L D,L-CBHcy completely inhibited BHMT activity indicating, that D,L-CBHcy was a specific inhibitor of BHMT. To address whether BHMT inhibition caused …
-
Developmental and evolutionary analysis of betaine homocysteine methyltransferase genes
… synthesize homocysteine. Betaine-homocysteine methyltransferase (BHMT) and BHMT-2 methylate homocysteine to form methionine using betaine and S-methylmethionine, respectively. Betaine is produced de novo from choline and is also found in the diet, whereas S-methylmethionine is only made in …
-
Studies of a DNA modification methyltransferase from Rhodobacter sphaeroides
The DNA methyltransferases represent an interesting class of enzymes for the study of protein-DNA interactions due to their high specificity, structural simplicity, and biological importance. RsrI methyltransferase (M$\cdot$RsrI) from Rhodobacter sphaeroides recognizes duplex d(GAATTC) and deposits …
-
ARCHITECTURE AND RNA RECOGNITION BY THE EUKARYOTIC M6A METHYLTRANSFERASE COMPLEX
… In eukaryotes, m6A is installed by the m6A Methyltransferase Complex (MTC), a multi-protein assembly composed of seven subunits in humans and six in Saccharomyces cerevisiae. However, the complete composition and architecture of the active MTC remain incompletely defined. This dissertation …
-
Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
… The genomic localization of de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) is facilitated in part by chromatin "reader" domains to establish DNA methylation patterns genome-wide. Recent work has highlighted a role for the PWWP reader domain in directing recruitment of DNMT3B …
-
Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer
<p>Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer Fabian Alejandro Mendoza Galvan Advisory Professor: Angela H. Ting, Ph.D. Fusobacterium nucleatum animalis (Fna) is found in the human oral cavity and gut. A distinct clade of Fna is primarily enriched in the …
-
Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
… T77 phosphorylation activates cap methyltransferase activity in vitro. Furthermore, it negatively regulates the interaction of RNMT with KPNA2 (Importin-a), which was found to inhibit RNMT activity in vitro. RNMT T77 phosphorylation is required for normal cell proliferation …
-
Thiopurine methyltransferase phenotyping and genotyping in clinical practice preferred access arrangement
Thiopurine methyltransferase (TPMT) metabolises the widely prescribed thiopurine drugs. The activity of this enzyme varies between individuals and this can influence treatment success. Rapid and accurate assays of TPMT and 6TGN, the active thiopurine metabolites, are increasingly recognised as …
-
Impact of the DNA methyltransferase inhibitor, 5-azacytidine, on chondrocytic phenotype
There is increasing amount of data indicating that epigenetics play a role in aging and aging-related diseases. Of the age-related disease, osteoarthritis is of particular importance to current health issues. Osteoarthritis (OA) is a progressive, degenerative disease that affects articular …
-
The Effects of Methyltransferase Genes on Human Haematopoietic Stem Cell Function
… human HSCs. In this thesis, I have focused on 2 methyltransferase genes, PRMT5 and DNMT3A, which are both epigenetic modulators of HSC function. Pharmacological inhibition of PRMT5 in healthy human cord blood (hCB) haematopoietic stem and progenitor cells (HSPCs) leads to reduced HSPC …
-
Screening for thiopurine s-methyltransferase (TPMT) gene mutations in South Africa
Several studies have shown that patients with low TPMT activity are at risk for severe and potentially fatal haematopoietic toxicity when treated with conventional doses of thiopurine drugs. Genetic polymorphism in the TPMT gene is an important determinant of mercaptopurine toxicity. Patients with …
-
Regulation of the histone H3K36 methyltransferase Set2 by the histone chaperone Spt6
… by Set2, an ortholog of the mammalian H3K36 methyltransferase SETD2. Several studies have shown that Set2 activity in vivo requires the histone chaperone Spt6. Genetic studies in yeast suggested that Spt6 regulation occurs via controlling a Set2 autoinhibitory domain (AID) and recent …
Page 1 of 17