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Showing 1 to 8 of 8 for “"Methylmalonyl-CoA Mutase"”.
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Studies on Methylmalonyl-CoA Mutase from Escherichia coli
Methylmalonyl-CoA mutase (MCM, E.C. 5.4.99.2), a coenzyme B12-dependent enzyme, catalyses the inter conversion of succinyl-CoA and methylmalonyl-CoA. The gene (sbm) encoding this enzyme is found in Escherichia coli (E. coli) at 62.3min on the E. coli chromosome. However, the metabolic role of this …
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Investigation of a novel intein-based Escherichia coli expression system for human methylmalonyl CoA mutase : a thesis presented to Massey University in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry
Human methylmalonyl CoA mutase (hMCM) is a 78 kDa homodimeric mitochondrial matrix enzyme. hMCM catalyses the conversion of 2R-methylmalonyl CoA to succinyl CoA in the metabolism of propionyl groups, and requires the vitamin B12 -derived cofactor adenosylcobalamin (AdoCbl). The mechanism of …
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Mutationsanalyse bei 21 mut°-Patienten mit Methylmalonylazidurie (MMA)
Bei der Methylmalonylazidurie (MMA) handelt es sich um eine autosomal-rezessiv vererbte Stoffwechselerkrankung, hervorgerufen durch einen Defekt der Methylmalonyl-CoA Mutase (MCM). In dieser Arbeit wurden 21 Patienten mit der mut°-Form auf Mutationen im MCM-Gen untersucht. Es wurden elf neue und …
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Konstruktion eines bakteriellen Systems zum Export von Coenzym B<sub>12</sub>
… bzw. der B12-bindenden Untereinheit (MutB) der Methylmalonyl-CoA-Mutase aus E. coli fusioniert. Zur Detektion der gebildeten Fusionsproteine wurden an das 3`-Ende der Gene die Sequenz für einen His6- oder Strep-Tag angefügt. Western-Blot-Analysen zeigten nur eine geringe Produktion des …
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Roles of the Methylcitrate and Methylmalonyl-COA Pathways in Mycobacterial Metabolism and Pathogenesis
… sources in vitro and can all generate propionyl-CoA. The methylcitrate cycle, which, in M. tuberculosis, uses a bifunctional isocitrate lyase/methylisocitrate lyase (ICL/MCL), is one of the two routes for metabolism of propionyl-CoA. A mutant strain of M. tuberculosis lacking the ICL/MCL was …
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Investigating cofactor transfer for a B₁₂-dependent enzyme
… (AdoCbl), has only one cognate enzyme in humans: methylmalonyl-CoA mutase (MCM), which is involved in the catabolism of several amino acids, cholesterol, and odd-chain fatty acids. MCM relies on two other proteins, a G-protein metallochaperone called methylmalonic aciduria type A protein (MMAA) …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
… This thesis focuses on the maturation of methylmalonyl-CoA mutase (MCM). In humans, MCM is the only known adenosylcobalamin (AdoCbl)- dependent enzyme; mutations or deletions of MCM or any metallochaperones involved in its maturation lead to methylmalonic aciduria, an inborn error of …
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Investigating the provision of vitamin B₁₂ in the Micro-Ecological Life Support System Alternative (MELiSSA) loop for long-distance space exploration using algal-bacterial cocultures.
… in central metabolism, methionine synthase and methylmalonyl CoA mutase. It is a corrinoid molecule, composed of a modified tetrapyrrole ring- with a cobalt ion at the centre and various possible upper and lower axial ligands. Vitamin B₁₂ is synthesised only by certain prokaryotes and the …