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Showing 1 to 9 of 9 for “"Methanosarcina thermophila"”.
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Studies on two nickel-containing enzymes from Methanosarcina thermophila TM-1
… protein content of acetate- and methanol-grown Methanosarcina thermophila was examined by two-dimensional polyacrylamide gel electrophoresis to determine the extent of regulation by the growth substrate. More than 100 mutually-exclusive spots were present in acetate- and methanol-grown cells …
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Studies on cytochromes and electron transport in Methanosarcina thermophila strain TM-1
Methanosarcina are methanogens capable of growth and methanogenesis from H₂/CO₂, formate, methanol, methylamines, and acetate. Methanosarcina conserve energy by coupling electron transport and methyl transfer to the generation of ion gradients during acetoclastic growth. This work focuses on …
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Biochemical characterization of a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… (Isf) from the acetate utilizing methanoarchaeon Methanosarcina thermophila was heterologously produced in Escherichia coli, purified to homogeneity, and characterized to determine the properties of the iron-sulfur cluster and FMN. Chemical and spectroscopic analyses indicated that Isf contained …
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Activation of acetate in acetate-grown Methanosarcina thermophila: purificationm and characterization of acetate kinase
Extracts of acetate-grown <u>Methanosarcina thermoghila</u> were assayed for the presence of enzymes which might catalyze a proposed activation of acetate as the initial step in the pathway of methanogenesis from acetate by that organism. Acetate kinase and phosphate acetyltransferase activities of …
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Molecular biology and biochemical characterization of the CO dehydrogenase-linked ferredoxin from Methanosarcina thermophila strain TM-1
… ferredoxin from acetate-grown <i>Methanosarcina thermophiIa</i> was characterized to determine the structure and biochemical properties of the iron-sulfur clusters. Chemical and spectroscopic analyses indicated that the ferredoxin contained two [4Fe-4S] clusters per monomer of …
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Studies on the carbon monoxide dehydrogenase enzyme complex present in acetate-grown Methanosarcina thermophila strain TM-1
… complex was purified from acetate-grown Methanosarcina thermophila. This complex made up greater than 10% of the cellular protein and the native enzyme formed aggregates with a Mr of approximately 1,000,000. The enzyme contained five subunits of different molecular weight suggesting a …
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Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
Carbonic anhydrase (CA) from acetate-grown Methanosarcina thermophila strain TM-1 was purified> 10,OOO-fold (22% recovery) to apparent homogeneity and a specific activity of 4,900 units mg⁻¹.The gene encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced. …
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Characterization of the genes and gene products of the acetate-activating enzymes and a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… kinase and phosphotransacetylase enzymes from <i>Methanosarcina thermophila</i> were isolated from a genomic library on a fifteen kilobase fragment The genes are located adjacent to one another, with the phosphotransacetylase gene (<i>pta</i>) directly upstream of the acetate kinase gene …
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Protein Phosphorylation in Archaea
… genomic DNA from the methanogenic archaeon Methanosarcina thermophila. From the PCR reaction a fragment of DNA was isolated that encoded a portion of a protein phosphatase. Using this DNA fragment as a probe, the entire phosphatase gene was isolated. The amino acid sequence of the …