Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Methanosarcina acetivorans"”.
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Post-translational modifications of methyl-coenzyme m reductase in methanosarcina acetivorans
… able to methylate MCR in vivo when expressed in Methanosarcina acetivorans, an organism that does not normally possess this PTM. M. acetivorans strains expressing MgmA showed small, but significant, reductions in growth rates and yields on methylotrophic substrates. Structural characterization of …
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Genetic and Biochemical Analysis of Methyltransferase Gene Regulation in Methanosarcina Acetivorans C2A
… the operon mtaCB whereas mtaA is monocistronic. Methanosarcina spp. have three mtaCB (mtaCB1, 2 and 3) operons. In order to understand why these organisms maintain genes encoding isozymes we used the three mtaCB operons in M. acetivorans C2A as a model. Mutant analysis established that these …
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Type IA topoisomerases: a phylogenomic and biochemical analysis of Methanosarcina acetivorans C2A TopoIII
… these enzymes have distinct biological roles. Methanosarcinales is the only sequenced archaeal order with this distinct distribution of two TopoIIIs. And yet, there is little information currently available on archaeal TopoIII. Here we report on Methanosarcina acetivorans TopoIIIα (MacTopoIIIα) …
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Genome scale metabolic reconstruction and hypothesis testing in the methanogenic archaeon Methanosarcina acetivorans C2A
Methanosarcina acetivorans strain C2A is a marine methanogenic archaeon notable for its substrate utilization, genetic tractability, and novel energy conservation mechanisms. To help probe the implications of this organism’s unique metabolism, we have constructed and manually curated a genome-scale …
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Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
In our study, we characterized DinB and found that DinB was able to synthesize through abasic DNA and cyclobutane pyrimidine dimers, and these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an …
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Expanding the genetic toolbox for methanogenic archaea and investigating ferredoxin redox circuit formation in Methanosarcina acetivorans
… electron donor for phospholipid saturation in Methanosarcina acetivorans, enhancing our understanding of ferredoxin dependent redox circuits and lipid biosynthesis in methanogens. In Chapter 3, I report the sat gene as a novel positive selectable marker for Methanosarcina spp. This work has …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
… demonstrated that the three distinct RPAs in M. acetivorans have different effects on DNA synthesis, strand exchange in homologous recombination, and flap endonuclease activity. This finding suggests that the Methanosarcinales might possess a ""division of labor"" among their three RPM in DNA …
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Analysis of the MT1/MT2 Systems Involved in the Metabolism of One-Carbon Compounds in Methanosarcina Acetivorans C2A
Embargo set by: Seth Robbins for item 72686 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs
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Top-Down Proteomics: Separation and Mass Spectral Characterization of Complex Cell Lysates From Methanosarcina Acetivorans and Human Cancer Cells
The combination of nano-LC-MS/MS with an online-offline automation platform and an iterative data search strategy has allowed for high-throughput data acquisition to be possible with top-down protein analysis. Taken together, these platforms represent the next step in increasing the utility of …
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Genetic and Biochemical Characterization of Methanol Utilization in Methanosarcina Acetivorans C2A: Analysis of the Methanol:methyl-Coenzyme M Methyltransferase System Encoded by mtaCB and mtaA
… analysis of the methylotrophic methanogenesis in Methanosarcina acetivorans has revealed a complex system for the utilization of methanol. M. acetivorans contains three isozymes of mtaCB and two of mtaA, encoding methyltransferase 1 (MT1) and methyltransferase 2 (MT2), respectively, which activate …
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Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
… the first high-throughput proteome analysis of Methanosarcina acetivorans utilizing a custom FT mass spectrometer. It outlines a two dimensional separations platform that utilizes a 96 well plate nanospray robot to facilitate automated analysis on the mass spectrometer. This thesis also …
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Computer-assisted generation and curation of genome-scale metabolic models with case studies in the methanogen genus Methanosarcina
… methanogens such as Methanococcus maripaludis, Methanosarcina acetivorans and Methanosarcina barkeri. Methanosarcina species are particularly attractive model organisms for methanogenesis due to their wide substrate utilization capabilities (compared to other methanogens): the diversity in …
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Functional and mechanistic elucidation of peptide backbone thioamidation
… the ribosome. Genetic studies in the methanogen Methanosarcina acetivorans have linked the thioamide in MCR with ycaO and its neighboring gene tfuA, which has no known function. In this thesis, I report the in vitro thioamidation of an MCR-derived peptide using methanogenic YcaOs and inorganic …
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Electron Transport in Methanosarcina: Pathway Heterogeneity Within the Genus
… methanogenesis in the freshwater methanogen Methanosarcina barkeri is known to utilize hydrogenases to transfer electrons from ferredoxin to methanophenazine. This thesis characterizes electron transport in the marine methanogen Methanosarcina acetivorans. Genomic comparison of hydrogenase …
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Fabrication, solute transport characterization and biological application of a microfluidic gradient chamber
… strictly anaerobic methanogenic archaea species (Methanosarcina acetivorans) was grown in the MGC by introducing high salt media along the two boundary channels. The M. acetivorans produced a large amount of methane gas, which ultimately displaced the liquid media in the 1200-well array, rendering …
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Investigating the Distribution and Biosynthesis of Modified F<sub>430</sub> Cofactors in Methanogenic and Methanotrophic Archaea
… species, including Methanococcus maripaludis, Methanosarcina acetivorans, and Methanocaldococcus jannaschii. A novel variant reported here has an exact mass of 1008.3478, a similar absorption spectrum as unmodified F<sub>430</sub>, and associates with purified Mcr from M. acetivorans. Based on …
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
… further insight into the not fully understood Methanosarcina acetivorans clamp loader.
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Molecular and Biochemical Analyses of a Three-Subunit Euryarchaeal Clamp Loader Complex
… novel form of clamp loader from the euryarchaeon Methanosarcina acetivorans (Mac). The M. acetivorans clamp loader comprises two similar small subunits (MacRFCS1 and MacRFCS2) and one large subunit (MacRFCL). Since the simplest RFC is made up of two different subunits and the most complex form is …
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Physiological and phylogenetic studies of marine methanogenic bacteria
… indicated that this isolate is a new species. Methanosarcina acetivorans is the proposed specific epithet. This is the first report of an acetotrophic methane-producing species indigenous to marine sediments. A method is described for mass culturing this and other acetotrophic methanogens using …
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Single-molecule and ensemble studies of DNA replication system
… clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans, and then moved to the single-molecule level trying to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a real-time fluorescence assay, we discovered that RFC can assemble a …
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