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Showing 1 to 20 of 72 for “"Methanosarcina"”.
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Genomweite Transkriptionsanalyse von Methanosarcina mazei Gö1
… zu generieren, die das gesamte Transkriptom von Methanosarcina mazei Gö1 repräsentieren.Ferner wurde eine TOPO-Plasmidbank des Transkriptoms von Ms. mazei Gö1 erstellt, die es ermöglichte neue hoch spezifische PCR-Produkte zu generieren, und damit in Zukunft die Nachproduktion von …
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Metabolism of methylated sulfur compounds in methanosarcina
Made available in DSpace on 2017-08-10T20:32:59Z (GMT). No. of bitstreams: 4 FU-DISSERTATION-2017.pdf: 13288108 bytes, checksum: b85f4636d57f959f787ecbec5a2c6201 (MD5) He_Fu_Thesis_4.17.docx: 11015164 bytes, checksum: 69b561ec04f67d2873af48f5281b0ad5 (MD5) LICENSE.txt: 4202 bytes, checksum: …
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Mechanisms of proton translocation in Methanosarcina mazei Gö1
… Enzymsysteme des methanogenen Archaeons Methanosarcina mazei Gö1 analysiert werden: Die F420H2:Heterodisulfid-Oxidoreduktase, die H2:Heterodisulfid-Oxidoreduktase sowie eine protonentranslozierende Pyrophosphatase.Es konnte gezeigt werden, dass 2-Hydroxyphenazin den Elektronentransport …
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Analysis of Methylotrophic Methanogenesis in Methanosarcina Barkeri Fusaro
Methanogens are anaerobic microorganisms that are able to gain all of their energy for growth by converting a limited number of substrates to methane gas. Methylotrophic methanogenesis involves the disproportionation of methylated compounds, such as methanol, to methane and carbon dioxide. In this …
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Analysis of hydrogen metabolism in methanosarcina barkeri fusaro
Made available in DSpace on 2010-05-14T20:51:15Z (GMT). No. of bitstreams: 3 Kulkarni_Gargi.pdf: 26285517 bytes, checksum: 09a83a385fb35766510a0bf93e3d07d8 (MD5) 1_Kulkarni_Gargi.pdf: 26264601 bytes, checksum: bedca9c1fe77d00d0cde15df38d30446 (MD5) license.txt: 4064 bytes, checksum: …
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Hydrogenase utilization and regulation in species of methanosarcina
… organic carbon into CO2 and methane. Species of Methanosarcina are unique among methanogens due to a versatile metabolism that allows the use of a wide range of substrates through four overlapping methanogenic pathways. Two of the pathways require H2 as a substrate, while the others can use H2 as …
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Characterization of the thioredoxin system in Methanosarcina mazei
… of the group, and have several Trxs and TrxRs. Methanosarcina mazei is one such organism, whose thioredoxin system is composed of one NTR, two FTRs, and five Trx homologs. Characterization of the components of a thioredoxin system sets the basis to further explore its function. I have expressed …
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Electron Transport in Methanosarcina: Pathway Heterogeneity Within the Genus
… methanogenesis in the freshwater methanogen Methanosarcina barkeri is known to utilize hydrogenases to transfer electrons from ferredoxin to methanophenazine. This thesis characterizes electron transport in the marine methanogen Methanosarcina acetivorans. Genomic comparison of hydrogenase …
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An updated genome scale metabolic reconstruction of methanosarcina barkeri
Methanosarcina barkeri is a methanogen, meaning that methane is the reduced product for energy generation. M. barkeri can grow on several substrates including methanol, acetate, methyl amines and a combination of hydrogen and carbon dioxide. The Methanosarcina barkeri genome was published in 2006 …
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Insights into substrate utilization and transcriptional regulation in methanosarcina
… to methane. Among methanogenic archaea, Methanosarcina are the most metabolically diverse being able to use H2/CO2, acetate, methanol, methylamines and methyl sulfides as substrates for methanogenesis. This dissertation presents biochemical and genetic studies on pyruvate utilization in …
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Post-translational modifications of methyl-coenzyme m reductase in methanosarcina acetivorans
… able to methylate MCR in vivo when expressed in Methanosarcina acetivorans, an organism that does not normally possess this PTM. M. acetivorans strains expressing MgmA showed small, but significant, reductions in growth rates and yields on methylotrophic substrates. Structural characterization of …
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Studies on two nickel-containing enzymes from Methanosarcina thermophila TM-1
… protein content of acetate- and methanol-grown Methanosarcina thermophila was examined by two-dimensional polyacrylamide gel electrophoresis to determine the extent of regulation by the growth substrate. More than 100 mutually-exclusive spots were present in acetate- and methanol-grown cells …
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Genetic and Biochemical Analysis of Methyltransferase Gene Regulation in Methanosarcina Acetivorans C2A
… the operon mtaCB whereas mtaA is monocistronic. Methanosarcina spp. have three mtaCB (mtaCB1, 2 and 3) operons. In order to understand why these organisms maintain genes encoding isozymes we used the three mtaCB operons in M. acetivorans C2A as a model. Mutant analysis established that these …
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Studies on cytochromes and electron transport in Methanosarcina thermophila strain TM-1
Methanosarcina are methanogens capable of growth and methanogenesis from H₂/CO₂, formate, methanol, methylamines, and acetate. Methanosarcina conserve energy by coupling electron transport and methyl transfer to the generation of ion gradients during acetoclastic growth. This work focuses on …
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Type IA topoisomerases: a phylogenomic and biochemical analysis of Methanosarcina acetivorans C2A TopoIII
… these enzymes have distinct biological roles. Methanosarcinales is the only sequenced archaeal order with this distinct distribution of two TopoIIIs. And yet, there is little information currently available on archaeal TopoIII. Here we report on Methanosarcina acetivorans TopoIIIα (MacTopoIIIα) …
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Genome scale metabolic reconstruction and hypothesis testing in the methanogenic archaeon Methanosarcina acetivorans C2A
Methanosarcina acetivorans strain C2A is a marine methanogenic archaeon notable for its substrate utilization, genetic tractability, and novel energy conservation mechanisms. To help probe the implications of this organism’s unique metabolism, we have constructed and manually curated a genome-scale …
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Biochemical characterization of a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… (Isf) from the acetate utilizing methanoarchaeon Methanosarcina thermophila was heterologously produced in Escherichia coli, purified to homogeneity, and characterized to determine the properties of the iron-sulfur cluster and FMN. Chemical and spectroscopic analyses indicated that Isf contained …
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Activation of acetate in acetate-grown Methanosarcina thermophila: purificationm and characterization of acetate kinase
Extracts of acetate-grown <u>Methanosarcina thermoghila</u> were assayed for the presence of enzymes which might catalyze a proposed activation of acetate as the initial step in the pathway of methanogenesis from acetate by that organism. Acetate kinase and phosphate acetyltransferase activities of …
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Functional analysis of the GlnK1 protein of Methanosarcina mazei strain Gö1: Aspects of nitrogen regulation
… das GlnK1-Protein aus dem methanogenen Archeaon Methanosarcina mazei Stamm Gö1 umfassend zu charakterisieren und seine potentielle Rolle in der Regulation des Stickstoffmetabolismus aufzuklären. Das M. mazei GlnK1-Protein weist den typischen Tyrosin51-Rest auf, der in Bakterien stickstoffabhängig …
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Heterologe Überexpression, Reinigung, biochemische Charakterisierung und Untersuchungen zur Struktur der A1 ATPase aus Methanosarcina mazei Gö1
… ahaB, ahaD und ahaG des methanogenen Archäons Methanosarcina mazei Gö1 wurden in den Überexpressionsvektor pGEM-4Z hinter die lac und T7-Promotoren kloniert.Dieses Konstrukt wurde pTL2 genannt.pTL2 enthält zusätzlich 162 Bp stromaufwärts von ahaE 3.Die auf pTL2 lokalisierten Gene wurden …
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