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Showing 1 to 14 of 14 for “"Methanocaldococcus jannaschii"”.
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Methanocaldococcus jannaschii and the Recycling of S-adenosyl-L-methionine
… conserved. In the anaerobic archaeal organism Methanocaldococcus jannaschii, the salvage of SAH, MTA, and 5'-dA, proceeds first via deamination to S-inosylhomocysteine (SIH), methylthioinosine (MTI), and 5'-deoxyinosine (5'-dI). The annotated SAH hydrolase from M. jannaschii is specific for SIH …
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Identification and Characterization of the Enzymes Involved in Biosynthesis of FAD and Tetrahydromethanopterin in Methanocaldococcus jannaschii
… and identified from methanogens. We are using Methanocaldococcus jannaschii as a model organism of methanogens to understand and investigate pathways of coenzymes biosynthesis. Our laboratory is involved in establishing the chemical functions of hypothetical proteins that function in targeted …
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Non-Coding Genomics of Methanocaldococcus Jannaschii: A Survey of Promoters, Non-Coding RNA Genes, and Repetitive DNA Elements
… features of an archaeal genome, that of Methanocaldococcus jannaschii, in an effort to identify and characterize promoters, non-coding RNA genes, and novel repetitive DNA sequences. Genome-wide identification of transcription factor binding sites revealed archaeal core promoter elements, …
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Characterization of Transcriptional Components in Methanocaldococcus Jannaschii: An Integrative Study of Archaeal Basal Transcription Factors and Putative Cis Elements
… to investigate the putative cis elements in M. jannaschii. Regions upstream of orthologous genes from four related methanococcal genomes were collected and analyzed for conserved motifs. This method was applied to identify putative rRNA promoter, and revealed novel elements potentially involved …
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Investigating the Distribution and Biosynthesis of Modified F<sub>430</sub> Cofactors in Methanogenic and Methanotrophic Archaea
… maripaludis, Methanosarcina acetivorans, and Methanocaldococcus jannaschii. A novel variant reported here has an exact mass of 1008.3478, a similar absorption spectrum as unmodified F<sub>430</sub>, and associates with purified Mcr from M. acetivorans. Based on the exact mass, this molecule is …
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Characterization of the Reaction Cycle of MJ0796: A Model Archaeal Adenosine Triphosphate-Binding Cassette Transporter Nucleotide Binding Domain
… binding domains, MJ0796 and MJ1267, from Methanocaldococcus jannaschii the highly conserved Walker A, Walker B, and LSGGQ motifs were probed using site-directed mutagenesis. Catalytic carboxylate mutants, MJ0796-E171Q and MJ1267-E179Q, exhibited nucleotide-dependent dimerization upon …
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GUIDE RNA-DEPENDENT AND INDEPENDENT tRNA MODIFICATIONS IN ARCHAEA
… that Pus10 from two different archaea, <italic>Methanocaldococcus jannaschii</italic> (MjPus10) and <italic>Pyrococcus furiosus</italic> (PfuPus10) have differential activities towards ψ54 formation. Using the crystal structure of Human Pus10 as template, we created homology models of …
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Orthogonal pair-directed codon reassignment as a tool for evaluating the factors affecting translation in E. coli
… of two commonly used orthogonal pairs from Methanocaldococcus jannaschii (M. jannaschiiI) and Methanosarcina barkeri (M. barkeri) to measure the in vivo reassignment efficiency of 30 different sense codons to tyrosine in E. coli with a simple fluorescence-based screen. The suite of sense …
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Characterization of the thioredoxin system in Methanosarcina mazei
… archaea, and a major focus has been on Methanocaldococcus jannaschii, a deeply rooted archaeon that has two Trxs and one TrxR. My thesis research concerns the thioredoxin system of the late evolving members of the group which are exposed to oxygen more frequently than the deeply rooted …
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STRUCTURAL AND FUNCTIONAL STUDIES OF ARCHAEAL BOX C/D GUIDE RNA AND ROLE OF A PUTATIVE HUMAN PSEUDOURIDINE SYNTHASE, PUS10 IN APOPTOSIS
… Unlike the close sR-tMet homologs (sR8 from Methanocaldococcus jannaschii and sR49 from Pyrococcus abyssi), the Box C’/D’ motif of sR-tMet is neither a K-turn nor a K-loop. The introduction of proper K-loop in the Box C’/D’ motif (sR-tMet with K-loop) abolished its Cm34 modification function …
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A TALE OF TWO METHYLATION MODIFICATIONS IN ARCHAEAL RNAs
… tests. Using the COG1901 recombinant enzyme from Methanocaldococcus jannaschii (Mj1640), purified enzyme Pus10 from M. jannaschii and full-size tRNA transcripts or TØ-arm (17-mer) fragments as substrates, the sequential pathway of m1Ø54 formation in Archaea was reconstituted. The methylation …
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Die außergewöhnliche Diversität der der Proteolipide in Archaea: Multimere und monomere Rotoren mit sechs bis dreizehn Ionenbindestellen in A1AO-ATPasen
Zusammenfassung 1. Das für das Proteolipid aus Methanocaldococcus jannaschii kodierende Gen atpK wurde in E. coli DH5alpha und in dem Minizell-Produzenten E. coli DK6 exprimiert. Das Genprodukt wurde durch radioaktive Markierung nachgewiesen. 2. Aus den Membranen der thermophilen, hydrogenotrophen …
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Novel Electron Transfer Systems in Hyperthermophilic Methanogenic and Anaerobic Methanotrophic Archaea: F420-dependent Nitrite Reductase and [Fe-S] Cluster Assembling Thioredoxin
… functions. From an investigation employing Methanocaldococcus jannaschii, an ancient lineage hyperthermophilic methanogen and inhabitant of deep-sea hydrothermal vents, as a model organism, our laboratory has discovered three novel enzymes linked to the organism's survival strategies, an …
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Sulfite reductase and thioredoxin in oxidative stress responses of methanogenic archaea
… phylogenetically deeply-rooted methanogen, Methanocaldococcus jannaschii. Our analysis of two of these targets employing biochemical assays suggested that Trx is needed for reactivation of oxidatively deactivated enzymes in M. jannaschii. To our knowledge, this is the first report on the …