Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Metallochaperones"”.
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Catalytic Mechanism and Maturation of the Metalloenzyme Nitrile Hydratase
… is understood that activator proteins may act as metallochaperones, bringing the metal to the active site. Metallochaperones regulate potentially toxic, but essential metals in cells. By understanding NHase's maturation, we can not only apply the knowledge to NHase understanding and engineering, …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
… often relies on proteins known as metallochaperones that transport, modify, and/or insert the metallocofactor for its target metalloenzyme. This thesis focuses on the maturation of methylmalonyl-CoA mutase (MCM). In humans, MCM is the only known adenosylcobalamin (AdoCbl)- dependent …
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Investigation of Escherichia coli [NiFe]-Hydrogenase Maturation
… that protein-protein interactions between the metallochaperones afford layers of nickel selectivity. The protein complexes HypA-HypB and SlyD-HypB are modulated by the GTPase cycle of HypB and mediate the selective release of nickel over zinc. In addition, characterization of the metal-binding …
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Developing Analytical Tools to Study Copper Homeostasis in Biological Systems
… characterize the Cu(I) affinity of proteins and metallochaperones. To this end, we developed a family of high-affinity Cu(I) chelators and fluorescent probes based on phosphine-sulfide-stabilized phosphine (PSP) binding motifs featuring varying Cu(I) dissociation constants from the femto- to …
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Elucidation Of The Functional Role Of Pcbp-The Cytosolic Iron Chaperone Protein Family, In Cellular Iron Homeostasis
… bind iron with an affinity similar to other metallochaperones. Based on these data, we believe that the PCBPs are functional iron chaperones that deliver iron to ferritin, hence promoting maintenance of cellular iron homeostasis. The objective is to characterize the molecular details of the …
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An old cofactor in a new light : how nature handles and repurposes adenosylcobalamin
… of AdoCbl require specialized machinery such as metallochaperones. In this thesis, I illustrate these different facets of the biochemistry of AdoCbl. Using X-ray crystallography, I determined structures of IcmF, a fusion protein between AdoCbl-dependent isobutyryl-coenzyme A mutase and a …