Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Metabolic fluxes"”.
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Methodologies and analysis of metabolic flux in mammalian systems
<p>Quantification of metabolic fluxes has broad applications in studying metabolic physiology. Isotope tracing with heavy labeled carbon (13C labeled metabolic flux analysis, 13C-MFA) is a promising strategy due to its ability to compute metabolic fluxes from isotope tracing profiles without …
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Rationale Ansätze zur Prozessentwicklung eines Anti-EGF-IL2-Fusionsproteins mittels einer rekombinanten BHK-Zelllinie
… aspects for process development with the tool of metabolic flux analysis in mammalian cells.1. Classical process developmentLimitations of the main nutrients (glucose, glutamine, amino acids) and inhibitions of metabolites (lactate, ammonia) as well as cultivation parameters (osmolality, pH-value …
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Development of quorum-sensing circuits for metabolic flux control in Escherichia coli
Metabolic engineering seeks to reprogram microbial cells to efficiently produce value-added chemicals. Traditionally, this is achieved by overexpressing the production pathway and/or knocking out competing endogenous pathways. However, limitations in some pathways are more effectively addressed …
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Computational Modeling of Planktonic and Biofilm Metabolism
… description of intracellular metabolism (e.g., metabolic fluxes) of microorganisms. Therefore, in this study, I have applied computational modeling approaches (i.e., 13C assisted pathway and flux analysis, flux balance analysis, and machine learning) to both planktonic and biofilm cells for …
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Integrated characterization of cellular physiology underlying hepatic metabolism
The macroscopic metabolic phenotype of a cellular system, such as insulin resistance, is the result of the integration of many hundreds or thousands of preceding cellular events, which culminates in the cell's final response to a perturbation in the environment. The data provided by DNA microarrays …
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Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
Metabolic Flux Analysis (MFA) has emerged as a tool of great significance for metabolic engineering and the analysis of human metabolic diseases. An important limitation of MFA, as carried out via stable isotope labeling and GC/MS measurements, is the large number of isotopomer equations that need …
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High resolution metabolic flux determination using stable isotopes and mass spectrometry
… are transcribed to proteins, which catalyze metabolic pathways, whose activity may in return affect gene expression. DNA microarrays allowed the measurement of the full gene expression profile under a particular set of environmental conditions and genetic backgrounds. To understand, however, …
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Quantifying pelagic primary production and respiration via an automated in-situ incubation system
… estimates of marine primary production and metabolic state. The resulting lack of data in these systems hinders efforts in scaling and including primary production in predictive models. To bridge the gap, we developed and validated a novel automated water incubator that measures in-situ …
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Cll Metabolism Is Regulated By Prognostic Factors, Modulated By Stroma and Abrogated By Pi3K Inhibition
… Compared with proliferative B-cell lines, metabolic fluxes of oxygen and lactate were low in quiescent malignant B lymphocytes from CLL patients. Glycolysis (extracellular acidification rate, ECAR) was consistently low in CLL samples, but oxygen consumption (OCR) varied considerably. Higher …
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Examining metabolic vulnerabilities for cancer therapy
Metabolic reprogramming is essential for cancer cells to balance energetics, maintain redox homeostasis, and synthesize biosynthetic precursors. Many chemotherapeutics that target metabolism are essential components of standard cancer treatment regimens, arguing that there is a therapeutic window …
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Method Development of 13C, 1H and 31P Magnetic Resonance Spectroscopy for use in Traumatic Brain Injury
… processing and mathematical modelling of in vivo metabolic fluxes within the brain. The first attempt at using [2-13C]glucose to quantify the in vivo neuronal tricarboxylic acid (TCA) cycle flux (VtcaN ) in humans is shown, accompanied with a Monte-Carlo error analysis to give a feel for the …
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Development of Magnetic Resonance Spectroscopic Imaging Methods to Assess Tumour Metabolism
… these techniques allows quantitative mapping of metabolic fluxes. A fast 3D deuterium MRSI sequence for dynamic imaging of [6,6’-2H2]glucose metabolism was developed and used to map glycolytic flux in a murine tumour model, demonstrating heterogeneity in glycolytic rate an early decrease …
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Molecular biogeochemistry of modern and ancient marine microbes
… temporal shifts in enzyme levels can redirect metabolic fluxes. This thesis illustrates how molecular techniques can contribute to a systems-level understanding of biogeochemical processes, which will aid in reconstructing the past of, and predicting future change in, earth surface environments.
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Molecular Biogeochemistry of modern and ancient marine microbes
… temporal shifts in enzyme levels can redirect metabolic fluxes. This thesis illustrates how molecular techniques can contribute to a systems-level understanding of biogeochemical processes, which will aid in reconstructing the past of, and predicting future change in, earth surface environment
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Biogeochemical and phylogenetic signals of Proterozoic and Phanerozoic microbial metabolisms
… the contribution of microbial biomass and its metabolic fluxes is difficult, especially in spatially and temporally-remote environments. Microbes leave behind an often scarce, unidentifiable, or nonspecific record on geologic timescales. This thesis develops and employs novel geochemical and …
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Nutrient availability modulating physiology and pathogenicity of <i>Legionella pneumophila</i>
… pneumophila</i>, as manifested by increased metabolic activity, impaired energy coupling, and altered metabolic fluxes. In particular, the physiological versatility of <i>L. pneumophila</i> was demonstrated by a significant decrease in the iron content of biomass (6-fold increase in …
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Biogeochemical and phylogenetic signals of Proterozoic and Phanerozoic microbial metabolisms
… the contribution of microbial biomass and its metabolic fluxes is difficult, especially in spatially and temporally-remote environments. Microbes leave behind an often scarce, unidentifiable, or nonspecific record on geologic timescales. This thesis develops and employs novel geochemical and …
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¹³C-metabolic flux analysis of recombinant yeasts for biofuels applications
… lignocellulosic material or VFAs will require metabolic engineering of biocatalysts to improve yields, productivities, and final titers. These metabolic engineering efforts can be facilitated by ¹³C-Metabolic Flux Analysis (MFA), a method for elucidating the otherwise unobservable intracellular …
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A Study of Cellular Amino Acid Homeostasis Identifies Novel Targets, Inhibitors and Combination Therapies in Cancer
… of transmembrane transport processes, metabolic pathways, protein synthesis and turnover, and signalling systems to furnish cells, tissues, organs, and organisms with optimal levels of these nutrients. Understanding how these elements support amino acid homeostasis in cancer cells is …
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A Study of Cellular Amino Acid Homeostasis Identifies Novel Targets, Inhibitors and Combination Therapies in Cancer
… of transmembrane transport processes, metabolic pathways, protein synthesis and turnover, and signalling systems to furnish cells, tissues, organs, and organisms with optimal levels of these nutrients. Understanding how these elements support amino acid homeostasis in cancer cells is …
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