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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 36 for “"Metabolic enzyme"”.

  1. Using human metabolic enzyme profiling as an innovative technology in the drug development process

    Sato, Hiroaki, 1962-Massachusetts Institute of Technology2000

    … unsuccessful due to species differences in the enzymes involved in drug metabolism. Recent progress in molecular biology made it possible to develop in vitro drug metabolism systems using human metabolic enzymes, such as purified microsomes or expressed cytochrome P450. These systems are useful …

    mit Repository record

  2. The JBS-associated E3 Ligase UBR-1 Maintains Synaptic Glutamate Homeostasis and Regulates Neurotransmitter Balance

    Chitturi, Jyothsna2015

    … UBR-1-regulated signaling pathway - a glutamate metabolic enzyme GOT/GOT-1.2, a vesicular glutamate transporter VGlut3/EAT-4, and a glutamate-gated chloride channel GluCl/AVR-15 - all involved in glutamatergic synaptic transmission in this premotor circuit. These findings suggest that UBR-1 …

    toronto-retro Repository record

  3. Dynamic Regulation of Metabolism in Archaea

    Todor, Horia2015

    … environments, cells must produce the same metabolic outputs to thrive. Although much is known about the regulation of metabolism in the bacteria and the eukaryotes, relatively little is known about the regulation of metabolism in archaea. Previous work identified the winged helix-turn-helix …

    duke Repository record

  4. Structural and functional analysis of a phospho-dependent molecular switch: Rv1827 from Mycobacterium tuberculosis

    Nott, T.J.UCL (University College London)2009

    … binding of the FHA domain to each of three metabolic enzyme complexes regulates their catalytic activities but does not require priming phosphorylation. However, phosphorylation of a threonine residue within a conserved N-terminal motif of Rv1827 triggers its intramolecular association with …

    ucl Repository record

  5. Protein folding in crowded environments and living cells

    Dhar, ApratimUniversity of Illinois at Urbana-Champaign2011

    … spatial resolution. We study the folding of the metabolic enzyme phosphoglycerate kinase (PGK) in U2OS (human osteosarcoma) cells and observe variations in folding kinetics within a single cell. FReI experiments carried out on an ensemble of 30 cells reveal large variations in folding time from …

    uiuc Repository record

  6. Signal transduction pathways involved in skeletal muscle fibre type regulation

    Higginson, James MatthewUniversity of Central Lancashire2003

    … stimulation (CLFS) was assessed, via metabolic enzyme activity assays and NADH-TR staining, following blockade of the calcineurin signalling pathway. Metabolic enzyme assays and northern blots were employed to assess the changes in enzyme activities and MEC isoform expression levels …

    cent-lancashire Repository record

  7. Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation

    Harvey, Mordecai MicahVirginia Tech2017

    … dismutase 2 (SOD2), substrate metabolism, metabolic enzyme activity, heat stress markers, and pH. To recreate the inflammatory milieu observed in metabolic disease states we treated the myotubes with a low dose of 20 EU lipopolysaccharide (LPS). Following the 24-hour stimulation we observed …

    vt Repository record

  8. Study of the differential compartmentation and functions of hexokinase isoforms and implications for cancer metabolism

    Zhang, Zhe, Ph. D. Massachusetts Institute of TechnologyMassachusetts Institute of Technology2017

    … targeting cancer-specific isoforms of key metabolic enzymes is a very promising strategy. Elevated glucose consumption and lactate secretion under aerobic conditions is a hallmark of many cancer cells. One of the major contributors to this highly glycolytic phenotype is hexokinase 2 isoform …

    mit Repository record

  9. Mechanism of methoxychlor toxicity in mouse ovarian antral follicles

    Basavarajappa, MallikarjunaUniversity of Illinois at Urbana-Champaign2012

    … (E2) levels by altering steroidogenic and metabolic enzymes in the antral follicles. Given that MXC induces atresia after long term exposure, in part, by increasing the pro-apoptotic factor Bax and decreasing the anti-apoptotic factor Bcl2 in antral follicles, the present study also tested …

    uiuc Repository record

  10. Determining the Usefulness of Aerobic and Anaerobic Enzyme Assays as Proxies for Rockfish Ecological Data.

    Hudson, Erica MDigital Commons @ University of South Florida2008

    … (late summer, fall, winter, and spring) using metabolic enzyme assays. Enzymes used were lactate dehydrogenase (LDH), malate dehydrogenase (MDH), pyruvate kinase (PK), and citrate synthase (CS). Some muscle composition data (percent water, percent protein, percent lipid, and protein as a …

    usf Repository record

  11. Metabolic vulnerability in HER2-positive Breast Cancer

    Ding, Yi2018

    … lethal targets. TALDO1, a gene encoding a metabolic enzyme in the non-oxidative pentose phosphate pathway was identified from the screen. Metabolic profiling with isotope-labeled glucose was used to understand the mechanism. The profiling results indicated that TALDO1 was necessary for …

    duke Repository record

  12. Prediction of Metabolic Stability and Bioavailability with Bioisosteric Replacements

    Choy, Alison Pui KiUniversity of Cambridge2018

    … trials due to numerous issues, including metabolic stability and efficacy issues, wasting years of research effort and resource. This thesis detailed the development of in silico methods to predict the metabolic stability of structures and their bioavailability. Coralie Atom-based …

    cambridge Repository record

  13. Molecular Dissection of the Ubiquitin-Independent Degradation Signal At the Unstructured Amino Terminal End of Human Thymidylate Synthase

    Melo Carlos, Sandra Patricia

    … substrates is the human DNA metabolic enzyme thymidylate synthase (TS). Previous studies showed that the degradation of TS is mediated by an intrinsically disordered 27-residue region at the N-terminal end of the protein, and that this region, in cooperation with an …

    south-carolina Repository record

  14. Therapeutic Potential of Prmt5 and Mat2A As Synthetic Lethal Targets In Mtap-Deficient Gbm Tumors

    Barekatain, Yasaman, <p>0000-0002-4454-1496</p>2024

    … lung squamous cell carcinoma. MTAP is a critical metabolic enzyme in the methionine salvage pathway responsible for the breakdown of methylthioadenosine (MTA). As a result, <em>MTAP</em>-deleted cells cannot metabolize MTA, leading to the accumulation of MTA. High levels of MTA in …

    uthsc Repository record

  15. Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases

    Dharmarajan, LakshmiVirginia Tech2010

    … (Pepc) are two important CO₂-fixation enzymes which share a similar reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While PEPCK is an important metabolic enzyme in animals and plays a broad …

    vt Repository record

  16. Physiological adaptations in mdx mice treated with microdystrophin gene therapy and endurance exercise

    Hamm, Shelby ElizabethVirginia Tech2022

    … ex vivo diaphragm and soleus force, as well as metabolic enzyme and mitochondrial respiration assays were differentially improved, revealing unique physiological adaptations to each microdystrophin construct. Together, the data demonstrated that response to exercise after gene therapy treatment …

    vt Repository record

  17. PLASTICITY IN PROTEIN SEQUENCE-FUNCTION RELATIONSHIPS

    He, Chenlu2021

    … (BirA) system, which serves as both an essential metabolic enzyme and a transcription regulator. In its repressor function, binding to the vitamin biotin allosterically activates BirA dimerization and the resulting repression complex assembly on the biotin operator sequence. Although the …

    maryland Repository record

  18. Studies Of The Molecular Mechanisms Underlying Cancer Stem Cells And The Transformed Metabolic Phenotype

    Sullivan, KellyCornell University2015

    … and the GTP-binding protein/crosslinking enzyme tissue transglutaminase (tTG), two proteins suspected to have key roles in tumor initiation and the development of the malignant state, in glioma stem cells (GSCs). Additionally, I examined the contributions of two isoforms of glutaminase …

    cornell Repository record

  19. The role of NEDD9 in TGFβ mediated tumour initiating cell dynamics in the Claudin-low breast cancer subtype

    Greenwood, WendyUniversity of Cambridge2021

    … interactor identified in this subtype is the metabolic isoenzyme PKM2. Through a variety of techniques, I explore the known roles of PKM2 in the regulation of oncogenic metabolic reprogramming and demonstrate how these processes are influenced by its association with NEDD9. Finally, I …

    cambridge Repository record

  20. Microfluidic concentration-enhanced single cell enzyme activity assay

    Sarkar, AniruddhMassachusetts Institute of Technology2013

    … platform for the direct measurement of enzyme activities from selected single cells without disrupting their extracellular context. We develop modules to: enhance enzyme assay sensitivity by microfluidic confinement, interface microfluidic devices with selected single cells, enable …

    mit Repository record

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