Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Metabolic burden"”.
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CRISPR-based Control of Biosynthetic Pathways and Genetic Biosensors, Informed by a Quantitative Investigation of Metabolic Burden
… engineered control systems to avoid overly burdening the host. Optimizing the genetic controllers of pathway enzymes, for example, achieves greater likelihood of acceptable balance between cost and production, avoiding reductions in growth rate, population homogeneity, and production titers …
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Building a robust bacterial chassis for synthetic biology
… could be exploited to develop a new class of metabolically efficient, contamination resistant host organisms for industrial biotechnology. {\it E.coli} cultures were exposed to a nutritionally stressful scenario by aging them in fed-batch cultures. Upon competing the aged cultures with the …
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USING ANTISENSE MESSENGER RNA TO DOWNREGULATE LON MEDIATED PROTEOLYSIS IN ESCHERICHIA COLI
The advent of metabolic engineering has instigated the introduction of foreign heterologous proteins into host cells, such as Escherichia coli. However, the metabolic burden incurred by the host cell to produce the desired recombinant protein elicits a cellular stress response that can result in …
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Multiplexed transcriptional control strategies for biosynthesis from mixed substrates in Escherichia coli
Metabolic engineering reprograms microbes to produce value-added chemicals. Microbial production has the potential to use renewable feedstocks, such as conventional waste streams. Metabolic engineers already contend with the metabolic burden of recombinant production pathways; utilizing complex …
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The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli
… in Escherichia coli, however, put an increasing burden on protein synthesis and processing. This can result in the production of improper or misfolded forms of protein that are degraded by host cell proteases. Proteolytic degradation may be enhanced by cellular stresses caused by the metabolic …
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Advancing Tools for Quantifying and Engineering Microbial Consortia
… interactions. For example, although metabolic pathways are often engineered in single microbial populations, the introduction of heterologous circuits into the host can create a substantial metabolic burden that limits the overall productivity of the system. This limitation could be …
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Multiscale modeling of microbial communities
… competition and the fitness cost due to metabolic load. Interestingly, under the naturally occurring scenario where bacteriocin production has a high cost, density-dependent synthesis is more advantageous than constitutive synthesis, which offers a quantitative interpretation for the wide …