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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 156 for “"Metabolic Engineering"”.
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Multigene Metabolic Engineering Via The Chloroplast Genome
… are encoded polygenetically. Chloroplast genetic engineering offers an alternate approach to multigene engineering by allowing the insertion of entire pathways in a single transformation event, while being an environmentally friendly approach. Stable integration into the chloroplast genome and …
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Integrating manual and automated metabolic engineering methods
Made available in DSpace on 2016-07-07T20:27:15Z (GMT). No. of bitstreams: 4 RICHARDS-DISSERTATION-2016.pdf: 3860821 bytes, checksum: cbaf30718ce7594d7f76859b49a220de (MD5) Richards_Matthew.docx: 2576729 bytes, checksum: e2a1076a288d7d31c646df244282d03a (MD5) LICENSE.txt: 4213 bytes, checksum: …
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Enabling Cl-Based bioconversion with metabolic engineering
… for their genetic modification. This leaves the metabolic engineer with a choice: to develop genetic tools to enable engineering in the desired host, or to import the relevant catabolic pathway into a more tractable organism, such as Escherichia coli. This thesis explores both options within the …
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Metabolic engineering analysis of post-burn hepatic hypermetabolism
Metabolic engineering refers to the directed improvement of product formation or cellular properties through the modification of specific biochemical reactions or introduction of new ones with the use of recombinant DNA technology. It has been used to investigate and modify intermediary metabolism …
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Development and application of computational tools for metabolic engineering
… successful in streamlining the design and engineering of different systems in many areas of science and engineering. However, due to its complex nature, modeling in biology is in an early stage and many more years of development is required to achieve the level of confidence that exists …
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Metabolic engineering of amino acid production in Corynebacterium glutamicum
… Institute of Technology, Dept. of Chemical Engineering, 1995.
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Metabolic engineering of yeast for xylose uptake and fermentation
… of lignocellulose. Rational and combinatorial metabolic engineering approaches coupled with transcriptomic and proteomic studies have been extensively exploited on the yeast Saccharomyces cerevisiae for improved xylose utilization. However, the resulting strains remain inapplicable for …
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Combinatorial search strategies for the metabolic engineering of microorganisms
Although the field of microbial metabolic engineering has traditionally been dominated by rational and knowledge-driven approaches, recent advances in genetic engineering have led to the emergence of a new methodology based on phenotypic diversification and screening. Unlike "classical strain …
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Systems metabolic engineering of Arabidopsis for increased cellulose production
… had not made. Among the many methods, predicting metabolic functions with genome-scale models had shown promising results in the recent years. Using sophisticated algorithms, such as flux balance analysis, OptKnock, and OptForce, we can predict flux distributions and design metabolic engineering …
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Metabolic engineering of microorganisms for the overproduction of fatty acids
… biosynthetic pathways, thus complicating the engineering for higher yields. With the advent of systems metabolic engineering, we demonstrated an iterative metabolic engineering effort that integrates computationally driven predictions and metabolic flux analysis (MFA) was demonstrated to meet …
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Metabolic engineering of xylose-fermenting saccharomyces cerevisiae for bioisoprene production
… synthesis of isoprene has been carried out by metabolic engineering that is environmentally-being application of commodity chemical production. Concerning the CO2 release for isoprene production, through petrochemical production methods, lured scientist into ruminating over bioisoprene …
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Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
Forward metabolic engineering (FME) is a rational approach to cellular engineering, relying on an understanding of the entire metabolic network to direct perturbations for phenotype improvement. Conversely, inverse metabolic engineering (IME) uses a global, combinatorial approach to identify …
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Metabolic engineering strategies for increasing lipid production in oleaginous yeast
… of this work is to use the tools of rational metabolic engineering to improve lipid production in the non-conventional oleaginous yeast Yarrowia lipoytica on two representative carbon sources, glucose and acetate. Previous work in this area achieved considerable success with the simultaneous …
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Metabolic engineering of C. glutamicum for amino acid production improvement
A central goal in metabolic engineering is the design of more productive biological systems by genetically modifying metabolic pathways. In this thesis we report such an optimization in the bacterial strain Corynebacterium glutamicum that is employed for the fermentative production of various amino …
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Metabolic Engineering of oleaginous yeast for the production of biofuels
… in crop-based production. Towards this end, metabolic engineering has established itself as an enabling technology for biofuels development. In this work we investigate the strategies of metabolic engineering for developing a biodiesel production platform, utilizing the oleaginous yeast …
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Computational methods for integrating metabolomics data with metabolic engineering strain design
… provides data ideal for applications in metabolic engineering. However, many of the computational tools for strain design are built around Flux Balance Analysis (FBA) and were developed using assumptions that preclude direct integration of metabolomics data into the underlying models. To …
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Metabolic Engineering and Transhydrogenase Effects on NADPH Availability in Escherichia coli
The ultimate goal in the field of metabolic engineering is improving cellular processes in a rational manner using engineering design principles and molecular biology techniques. The syntheses of several industrially useful compounds are cofactor-dependent. The reducing equivalent NADPH is required …
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Development of glucose valves for metabolic engineering applications in E. coli
… One outstanding challenge, however, is the engineering of these systems for economic viability. As a solution to this issue, a metabolite valve is proposed: a biochemical device which may be used to dynamically redirect metabolite flux away from endogenous processes into production pathways. …
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Metabolic engineering for the production of functionalized terpenoids in heterologous hosts
… in heterologous microbial systems through metabolic engineering stands as an attractive alternative to truly natural production and allows for increased control over biosynthetic pathways enabling improved yields, titer, and productivity. The anti-cancer molecule Taxol (Paclitaxel) stands …
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