Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 74 for “"Metabarcoding"”.
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Algal Biodiversity Survey of the Middle Chattahoochee River Using DNA Metabarcoding
… naked eye, so researchers have been using DNA metabarcoding, where DNA belonging to specific taxa can be isolated and identified from water samples. The objective of this study was to conduct an algal biodiversity survey on the middle Chattahoochee River system in Georgia. Water samples (1L) …
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Assessing variability in eDNA metabarcoding: implications for ecological interpretation in environmental monitoring
… slower. Scottish aquaculture has adopted eDNA metabarcoding into its environmental<br/>impact monitoring, providing a real-world example of eDNA use with standardisation gaps. This thesis focused on eDNA metabarcoding, primarily of the V3-4 region of the bacterial 16S gene. This thesis …
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Barcoding and eDNA metabarcoding of a rapidly changing Neotropical freshwater fish community
… bolster the implementation of DNA barcoding and metabarcoding in Brazilian riverine ecosystems. With the cooperation of collaborators in both Brazilian and British institutions, I built a barcode library and provided a more robust biodiversity record for the ichthyofauna of the Doce river, …
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From port to ballast water: application of ADN metabarcoding of shipborne biodiversity
… de células, heces, piel, saliva, moco, ...) y el metabarcoding (asignación taxonómica de individuos de una muestra ambiental basada en sus secuencias de ADN) se distinguen, en esta tesis, como dos posibles catalizadores para proporcionar datos biológicos al Convenio BWM. Los dos casos de estudio …
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Environmental DNA (eDNA) Metabarcoding: Analysis of Freshwater Macroinvertebrate Community Structure and Diversity
… are being explored. Environmental DNA (eDNA) metabarcoding, which can be used to identify the taxonomic composition of entire communities, has the potential to increase both survey efficiency and taxonomic resolution. In this study, I evaluated the efficacy of eDNA metabarcoding to document …
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Improving the applicability of metabarcoding to monitor salmon farming impacts on benthic environments
… can be overcome with environmental DNA (eDNA) metabarcoding. To utilise metabarcoding for regulatory monitoring, an understanding is needed of the sources of variability in metabarcoding-derived data. Metabarcoding-inferred indicators of impact also need to be identified specifically for fish …
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
… their ability to isolate eDNA for the purpose of metabarcoding a section of the ribosomal small subunit 18 S (18s) and the cytochrome oxidase I (COI) gene regions. The 18s sequence data is non-reportable due to lack of sequence quality, and MoBio's PowerWater did not yield DNA suitable …
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Exploitation of underexplored physiological, metabarcoding and evolutionary aspects to enhance invasive insect pest management
Invasive pests threaten agriculture and ecology of the invaded countries. To contrast and reduce their impact, entomologists develop specific and sustainable control strategies, which should target the pest and should not affect the surrounding environment. Therefore, a profound knowledge of pest …
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The role of sampling location and depth in the performance of lake plant environmental DNA metabarcoding
… from twelve temperate lakes via ITS1 rRDNA metabarcoding for aquatic and terrestrial plant taxonomic patterns across horizontal and vertical space. We detect the expected community variation (beta diversity) from lake to lake, but few community composition patterns related to offshore …
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Characterizing the Microbiome of Scottish Freshwater Pearl Mussels (Margaritifera margaritifera): A Metabarcoding Analysis using Next-Generation Sequencing
… microbial profiling conducted via 16S rRNA gene metabarcoding. Two sequencing platforms were utilized: the MinION (Oxford Nanopore Technology) for full-length amplicons and MiSeq (Illumina) for short amplicons, enabling a comparative analysis of platform performance at the species level. Results …
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Using environmental DNA metabarcoding to monitor fish biodiversity and assess anthropogenic barrier impacts on the river Mersey, UK
… molecular approach, environmental DNA (eDNA) metabarcoding, is now revolutionising biomonitoring. In this thesis, data collected from non-invasive monitoring techniques (camera recordings and eDNA metabarcoding) were combined to monitor Atlantic salmon migration at the Woolston weir fish pass, …
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Environmental DNA metabarcoding and citizen science as a cost-effective and rapid tool for monitoring terrestrial mammalian species
… rare, elusive and/or invasive species. eDNA metabarcoding (which targets multiple species simultaneously) has been frequently used to monitor aquatic species but more recently, has been applied to detect semi-aquatic and terrestrial mammals. eDNA, with the help of citizen science, enables …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… genus level. This project aimed to introduce DNA metabarcoding techniques to classify and identify grass and ragweed species contributing to pollen allergies in various South African biomes. Methods Samples were selected from weeks with the highest grass pollen counts between October 2022April …
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Metabarcoding pollen to identify allergenic species of grasses (Poaceae) and ragweed (Ambrosia spp.) across six monitored sites in South Africa
… genus level. This project aimed to introduce DNA metabarcoding techniques to classify and identify grass and ragweed species contributing to pollen allergies in various South African biomes. Methods: Samples were selected from weeks with the highest grass pollen counts between October 2022- April …
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Αλληλούχηση του barcoding γονιδίου COX1 σε είδη ασπονδύλων, υδατικών συστημάτων της Ελλάδας και δημιουργία πρότυπης βάσης αναφοράς για μελέτες metabarcoding και περιβαλλοντικού DNA (eDNA).
… όπως το περιβαλλοντικό DNA (eDNA) και το metabarcoding. Στην παρούσα μελέτη, παρήχθησαν αλληλουχίες του μιτοχονδριακού γονιδίου COI για υδρόβια Κολεόπτερα που συλλέχθηκαν από υδατικά οικοσυστήματα στην Ελλάδα. Η πλειοψηφία των ατόμων Κολεοπτέρων που μελετήθηκαν, προήλθαν κυρίως από τον …
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Development of Bioinformatic Tools and Procedures to Computationally Support DNA Barcoding
… alignments as part of DNA barcoding and DNA metabarcoding. In addition, the existing barcode regions were investigated to discover if possible mini-barcodes could be used in order to improve identification in a wide range of plant species. The pipelines developed in the project have also been …
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Nematodes as indicators of sustainable soil health management practices in corn-soybean production
… knowledge gaps by combining enhanced DNA metabarcoding tools with the standard morphological analyses to evaluate soil nematode communities under different crop management practices. The first study evaluated the use of soil DNA for profiling nematode communities. Compared to the commonly …
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Benthic meiofaunal and micro-eukaryotic inhabitation of extreme environments: Submerged caves and hydrocarbon seeps
… Peninsula and Cozumel Island, Mexico using metabarcoding analysis. Despite a large percentage of unidentified sequences, diverse protistan, metazoan, and fungal taxa were detected, spanning a range of feeding strategies, with community composition differing both between caves and within a …
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Using eDNA to construct community assemblages of amphibians at sites infected with Batrachochytrium salamandrivorans in the Netherlands.
… may cause. Utilising environmental DNA (eDNA) metabarcoding is a relatively new and effective method of monitoring biodiversity, especially elusive species. Thus the aims of this study are to 1.) validate the use of two different metabarcoding primers and single-species approaches in detecting …
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The use of environmental DNA for the detection of Palaemonias ganteri (Hay, 1901), a federally endangered cave species
… using traditional techniques. Advancements in metabarcoding and Next Generation Sequencing (NGS) technologies provide tools that may allow researchers and managers to address some of the hurdles posed by the difficult environment where P. ganteri lives. </p> <p>This study was able to identify …
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