Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Merozoite"”.
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Identifying Merozoite Targets of Protective Immunity Against <i>Plasmodium falciparum</i> Malaria
… adults. <br></br><br></br> First, to identify merozoite antigens that were immunogenic, I resolved proteins extracted from <i>P. falciparum</i> merozoites by two-dimensional gel electrophoresis and tested these for reactivity with immunoglobulins from malaria immune adults. Immunoreactive …
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Characterisation of the pre-invasion glycophosphatidylinositol-anchored surface proteins of Plasmodium falciparum merozoites
… disease. During the intraerythrocytic phase, merozoites egress from mature schizonts to invade new uninfected erythrocytes. Glycophosphatidylinositol (GPI) -anchored proteins cover most of the exterior surface of the merozoite prior to invasion, while other GPI-anchored proteins are released …
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New approaches to understanding the interactions between Plasmodium falciparum merozoites and human erythrocytes during invasion
… that is released from infected erythrocytes (merozoite), being able to invade a human erythrocyte and muliply. Invasion is a complex process involving multiple receptor-ligand interactions and multiple steps. It begins with attachment of the parasite to the erythrocyte, followed by …
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Defining Molecular Mechanism of Egress in Cryptosporidium
… understanding of gene function in the parasite. Merozoite egress is a crucial event in the life cycle of Cryptosporidium that contributes to parasite propagation. However, the molecular mechanisms behind egress remain unknown. We propose to study the genes upregulated in the intracellular stages …
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The Role of Antibodies to <i>Plasmodium falciparum</i> Merozoite Antigens in the Resistance of Young Infants to Febrile Malaria and Their Place as a Biomarker in the Detection of Malaria Transmission Hotspots
Over the last 15 years the malaria burden has globally declined, but not evenly across endemic areas. In areas with substantial decline, elimination became realistic. However, malaria elimination has to, in a cost-effective way, overcome problems such as increasing drug and insecticide resistance …
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Functional Characterization of Serine Hydrolases Mediating Lipid Metabolism and Protein Depalmitoylation in Asexual Stage Plasmodium Falciparum
… mainly focused on a depalmitoylase essential for merozoite invasion and lysophospholipases (LPLs) essential for acquiring fatty acids (FAs) from the host. Identifying essential metabolic enzymes will benefit the treatment to malaria. We focused on metabolic SHs and identified two SHs were …
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Static and Microfluidic Live Imaging Studies of Plasmodium falciparum Invasion Phenotypes
… all the symptoms and mortality of malaria. The merozoite, first invade a red blood cell (RBC) in the circulation, then grows, develops and multiplies within it by clonal division. Merozoite invasion is a complex process involving dynamic interactions between ligands in the merozoite coat and …
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Immune responses to polymorphic antigens and protection against severe malaria in Kenyan children
… antibodies to five leading <i>P.falciparum</i> merozoite stagevaccine candidate antigens, and schizont extract, in Kenyan children monitored longitudinally for mild and severe malaria. I also genotyped parasites from clinical episodes to investigate allele-specific antibody-mediated …
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Identification and characterisation of host-pathogen protein-protein interactions in the blood stages of malaria
… identified an interaction between P. falciparum merozoite protein 7 (PfMSP7) and human P-selectin (SELP). I used surface plasmon resonance and flow cytometry approaches to validate this interaction and, by screening more widely across the MSP7 protein family, identified that SELP-binding is a …
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Using biophysical tools to explore the erythrocyte preferences of the zoonotic malaria parasite Plasmodium knowlesi
… in human red blood cells, however with a reduced merozoite-erythrocyte attachment force. *P. knowlesi* had a longer duration internalisation and echinocytosis, which may be due to the larger size of merozoites in this species. Next, host cell physical parameters were assessed to investigate …
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Dissecting the impact of macrophage migration inhibitory factor (MIF) on host immune response
… EMIF, predominantly secreted from the invasive merozoite stage, may help the parasite exploit the host immune response by interacting with common ChMIF receptors. This may lead to functional mimicry thus provoking the question of whether EMIF would modulate the biological functions of ChMIF to …
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Proteomic Analysis Delineates the Signaling Networks of Plasmodium falciparum
… developmental stages where nuclear division and merozoite formation occur. Further analysis of the decreased phosphorylated events revealed three motifs that are enriched among phosphorylated sites in proteins that are down regulated. In vitro kinase assays were done to validate the potential …
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Untersuchungen zur zellulären und humoralen Immunantwort gegen das Merozoitenoberflächenprotein-1 des Malariaerregers Plasmodium falciparum
… In unserer Gruppe konzentrieren wir uns auf das Merozoitenoberflächenprotein-1 (merozoite surface protein 1, MSP-1), das Hauptoberflächenprotein des Blutstadiums von P. falciparum. MSP-1 gilt als einer der interessantesten Impfstoffkandidaten. Für die Entwicklung einer effektiven …
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Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5)
El conocimiento de las proteínas implicadas en el proceso de invasión de los merozoitos a los eritrocitos por Plasmodium es el punto de partida para el desarrollo de nuevas estrategias para controlar la malaria. Muchas de estas proteínas han sido estudiadas en Toxoplasma gondii, donde se han …
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Development of a plant-based vaccine against malaria
Malaria is the most prevalent tropical human disease reported worldwide, caused by protozoan parasites. Half of the world's population is at risk of malaria, and more than 200 million new cases are reported annually. Currently, there are no licensed vaccines available for use. Therefore, there is a …