Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Membraneless organelles"”.
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Linking Biomolecular Condensates to Disease and Therapeutic Development
… is compartmentalized into membrane-bound and membraneless organelles that organize and regulate key cellular functions. Over the past decade, growing evidence supports the notion that membraneless organelles, called biomolecular condensates, compartmentalize biomolecules – proteins and nucleic …
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Regulation of Pumilio RNA Binding Proteins by Long Noncoding RNA NORAD
… amount of PUM proteins into novel liquid-like membraneless organelles through a multivalency-induced phase separation mechanism. Moreover, we provide a molecular understanding of the properties of NORAD that promote PUM phase separation, revealing new principles of lncRNA function and …
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Equilibrium phase separation with many conserved order parameters - Analytical theory and applications in biomolecular condensation
… focus on applying new insights to understand membraneless organelles formed by proteins. There, the relevant order parameters are concentrations of various solute components, and the large number of interacting molecular species in physiological systems translates into a set of many …
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Leveraging Protein Homeostasis as a Disease-modifying Strategy
… can enable specificity in the formation of membraneless organelles formed via PPS. Based on this, we develop a method for predicting potential substrate-specific modulators of PPS targets in disease and apply it toward a proposed AD PPS target CAMKK2. Together, our findings contribute to a …
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The Effects of DEAD-box Helicase Activity on Biomolecular Condensate Structure and Dynamics
Biomolecular condensates are membraneless organelles which help spatiotemporally organize the biochemistry of the cytoplasm and nucleoplasm. These bodies are composed of specific proteins and RNA which self-associate to drive condensate formation. The strengths, timescales, and valence of the …
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Phase Transitions of Multivalent Adaptor Proteins
… paraspeckles, etc., were also described as membraneless organelles. Furthermore, membranes themselves are self-assembled entities of lipids, proteins and carbohydrates. Additionally, within and on surface of membranes, molecules cluster into signaling compartments in many different …
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Programmable Assembly of Elastin-like Polypeptides Within Droplet Microenvironments
… of a cell and form liquid non-membrane bound organelles. These compartments are thought to play crucial roles in various cellular processes such as ribonucleic acid maintenance and gene expression. While the physicochemical drivers and biophysical properties of intracellular organelles is …
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Multiscale coarse-grained models for biological phase separation: Development and applications
… phase separation (LLPS) in order to generate membraneless organelles, by which the cell can organise its biomolecules and bioprocesses dynamically in space and time. Many of the experimental and theoretical methodologies used to study the formation of these condensates still struggles to …
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Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
Biomolecular condensates (BCs) are membraneless organelles recently recognised as crucial regulators of numerous cellular processes, essential for cellular fitness and dynamic adaptation to environmental changes. They facilitate spatiotemporal control in critical cellular functions, including …
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RNA-MEDIATED PHASE SEPARATION OF 53BP1: CONNECTING CONDENSATE BIOPHYSICS TO DNA DAMAGE RESPONSE
Biomolecular condensates, also known as membraneless organelles, are intracellular compartments which can form via liquid-liquid phase separation (LLPS) where biochemical reactions concentrate within the cell. An example of such condensates are DNA damage response (DDR) foci, proteins and RNA …
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Thermodynamic signatures for hexapeptides with propensity for phase separation and amyloid formation
… The IDPs are also an important constituent of membraneless organelles that are formed by phase separation of proteins. Some IDPs can also aggregate and form amyloids. The occurrence of these amyloids in vivo is a hallmark of various neurodegenerative disorders. Phase separation and amyloid …
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A CORRELATIVE CRYO-ELECTRON TOMOGRAPHY APPROACH TO STUDY BRD4-NUT CHROMATIN CONDENSATES ACROSS SCALES
… a versatile platform generalizable to other membraneless organelles. In combination with SOLIST, this approach may be extended to patient-derived NUT midline carcinoma specimens, effectively enabling a “biopsy at the nanoscale” and offering a tool for direct molecular mapping of human …
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Characterization and Modulation of the Emergent Material Properties of Biomolecular Condensates
<p>The discovery of membraneless organelles has revolutionized the field of cell biology and our understanding of cell compartmentalization. These organelles lack a distinct phospholipid bilayer and interact with the cytoplasm mediated by a liquid-liquid interface. The emergent properties and …
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Non-equilibrium phase separation in active matter
… by studies in biomolecular condensates, or ‘membraneless organelles’, within cells. In contrast to their passive counterparts, such systems have conserved and non-conserved dynamics that do not, in general, derive from a shared free energy. This mismatch breaks time- reversal symmetry (TRS) …
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Importance of hierarchical interactions in concentrated protein systems
Protein-protein interactions play important roles in signal transduction, chemical catalysis, cell metabolism, membrane transport, muscle contraction and more. While many of these interactions can be understood as two-body interactions, other important properties can emerge when large numbers of …