Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Membraneless"”.
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The development of a membraneless direct borohydride alkaline fuel cell
… has been conducted on DBAFC, particularly membraneless DBAFC. This work aims to address this and describes an investigation into every aspect of DBAFC to evaluate their deployment on an industrial scale.As such, candidate electrocatalysts were assessed for their electrocatalytic activity …
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Membraneless hydrogen bromine laminar flow battery for large-scale energy storage
Electrochemical energy storage systems have been considered for a range of potential large-scale energy storage applications. These applications vary widely, both in the order of magnitude of energy storage that is required and the rate at which energy must be charged and discharged. One such …
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Linking Biomolecular Condensates to Disease and Therapeutic Development
… is compartmentalized into membrane-bound and membraneless organelles that organize and regulate key cellular functions. Over the past decade, growing evidence supports the notion that membraneless organelles, called biomolecular condensates, compartmentalize biomolecules – proteins and nucleic …
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Laminar Flow-Based Electrochemical Microreactors
… of nicotinamide cofactors for biocatalysis; (ii) membraneless fuel cells; and (iii) biofuel cells that utilize the ability to tailor the composition (e.g. pH) of the fuel and oxidant streams independently, which enables optimization of the reaction kinetics at the cathode and anode independently. …
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Microfluidic platforms for the investigation of fuel cell catalysts and electrodes
… with more degrees of freedom. Previously, membraneless microfluidic fuel cells have been developed to address some of the aforementioned fuel cell challenges (Chapter 2). At the microscale, the laminar nature of fluid flow eliminates the need for a physical barrier, such as a stationary …
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Targeting and Manipulating Endogenous Transcriptional Condensates
Biomolecular condensates are membraneless compartments composed of selectively concentrated biomolecules with liquid-like properties. In this thesis, we discuss our discoveries showing that biomolecular condensates are associated with transcription. However, tools to study transcriptional …
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Experimental investigation of integrated systems for biohydrogen and biomethane production
… system consists of a single-chamber cylindrical membraneless the microbial electrolysis cell (MEC), while the second system is an MEC-anaerobic digestion system. The system 1 investigates the effects of various parameters on the bioH₂ production, whereas the system 2 examines simultaneous …
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Regulation of Pumilio RNA Binding Proteins by Long Noncoding RNA NORAD
… amount of PUM proteins into novel liquid-like membraneless organelles through a multivalency-induced phase separation mechanism. Moreover, we provide a molecular understanding of the properties of NORAD that promote PUM phase separation, revealing new principles of lncRNA function and …
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Equilibrium phase separation with many conserved order parameters - Analytical theory and applications in biomolecular condensation
… focus on applying new insights to understand membraneless organelles formed by proteins. There, the relevant order parameters are concentrations of various solute components, and the large number of interacting molecular species in physiological systems translates into a set of many …
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β2-mikroglobulino gryninimas ir skysčio-skysčio fazių atsiskyrimo tyrimai /
… separate from the bulk solution to form dense, membraneless condensates. Although dozens of proteins have been identified to form condensates, there is currently no information on whether β2M can undergo LLPS. In the course of this work, the purification process of the protein was optimised. It …
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Leveraging Protein Homeostasis as a Disease-modifying Strategy
… can enable specificity in the formation of membraneless organelles formed via PPS. Based on this, we develop a method for predicting potential substrate-specific modulators of PPS targets in disease and apply it toward a proposed AD PPS target CAMKK2. Together, our findings contribute to a …
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The Effects of DEAD-box Helicase Activity on Biomolecular Condensate Structure and Dynamics
Biomolecular condensates are membraneless organelles which help spatiotemporally organize the biochemistry of the cytoplasm and nucleoplasm. These bodies are composed of specific proteins and RNA which self-associate to drive condensate formation. The strengths, timescales, and valence of the …
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Tools for Monitoring and Modulating Cellular Communication
… with our nanofluidic sampling platform for membraneless infusion and withdrawal of extracellular constituents at low flow rates. This allows for ‘liquid biopsies’ of the extracellular milieu and yields information on cellular signaling in healthy and diseased states in both in vitro and in …
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Phase Transitions of Multivalent Adaptor Proteins
… paraspeckles, etc., were also described as membraneless organelles. Furthermore, membranes themselves are self-assembled entities of lipids, proteins and carbohydrates. Additionally, within and on surface of membranes, molecules cluster into signaling compartments in many different …
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The role of DAXX in the selective autophagy receptor SQSTM1/p62 phase condensation
… clearance. Mounting evidence has revealed that membraneless compartments, participating in various biological processes, are assembled via liquid-liquid phase separation (LLPS). These phase-separated condensates ensure that cellular activities occur in a spatiotemporally controlled manner and …
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The evolution of centrosome and chromosome number in newly formed tetraploid human cells
… with double the number of centrosomes, the small membraneless organelle that organizes the cell's microtubule cytoskeleton and mitotic spindle apparatus. Centrosome number homeostasis is crucial for health, and recent studies have shown inducing extra centrosomes in cells can induce tumor …
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Programmable Assembly of Elastin-like Polypeptides Within Droplet Microenvironments
… addresses the need for engineered systems of membraneless organelles and consists of programming the assembly of elastin-like polypeptides, an archetypal class of disordered proteins, into model organelles within droplet microenvironments. We synthesized a library of recombinant elastin-like …
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Multiscale coarse-grained models for biological phase separation: Development and applications
… phase separation (LLPS) in order to generate membraneless organelles, by which the cell can organise its biomolecules and bioprocesses dynamically in space and time. Many of the experimental and theoretical methodologies used to study the formation of these condensates still struggles to …
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Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
Biomolecular condensates (BCs) are membraneless organelles recently recognised as crucial regulators of numerous cellular processes, essential for cellular fitness and dynamic adaptation to environmental changes. They facilitate spatiotemporal control in critical cellular functions, including …
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RNA-MEDIATED PHASE SEPARATION OF 53BP1: CONNECTING CONDENSATE BIOPHYSICS TO DNA DAMAGE RESPONSE
Biomolecular condensates, also known as membraneless organelles, are intracellular compartments which can form via liquid-liquid phase separation (LLPS) where biochemical reactions concentrate within the cell. An example of such condensates are DNA damage response (DDR) foci, proteins and RNA …
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