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Showing 1 to 20 of 86 for “"Membrane vesicles"”.
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Bacterial Outer Membrane Vesicles. Mediators of virulence and antibiotic resistance.
Outer membrane vesicles (OMV) have the size of nanoparticles and are released from the outer membrane of most Gram-negative bacteria. The OMV reflect the composition of the bacterial outer membrane, including lipids and virulence factors specific for the pathogen, and are involved in pathogenesis …
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Characterization of methionine transport in bovine intestinal brush border membrane vesicles
… of methionine uptake by brush border membrane vesicles of bovine small intestine were investigated. Alkaline phosphatase marked the brush border membrane fraction obtained through differential centrifugation followed by a sucrose gradient. This preparation yielded a 10-fold enrichment …
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Analysis and optimization of immune responses elicited by Outer Membrane Vesicles (OMVs)
Outer membrane vesicles (OMVs) are spheroidal particles released from all gram-negative bacteria and exert different biological functions. OMVs carry many microbe-associated molecular patterns (MAMPs) such as peptidoglycan, lipopolysaccharide (LPS), and lipoproteins and can be decorated with …
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Bacterial outer membrane vesicles (OMVs) as a platform for personalized cancer vaccines
… models and human patients. Bacterial Outer Membrane Vesicles (OMVs) are naturally produced by all Gram-negative bacteria. They contain several Microbe-Associated-Molecular Patterns (MAMPs), crucial for stimulating innate immunity and promoting adaptive immune responses. The ability to …
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Non-electrolyte transport in brush border membrane vesicles from bovine small intestine
… properties of bovine intestinal brush border membranes were investigated. Isolation of brush border membrane vesicles involved magnesium precipitation followed by a sucrose density gradient. Characterization by alkaline phosphatase activity (the brush border marker enzyme) showed 7 fold …
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Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles
… was studied through the use of brush border membrane vesicles (BBMV). Preliminary experiments determined that incubation in hyaluronidase is not necessary in order to separate mucosal tissue from the basement membrane and that the stop solution used in the uptake study needed to be buffered. …
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Virulence gene expression of three hypervirulent S. pyogenes M1T1 and membrane vesicles isolation
… and superficial infections of skin and mucosal membranes up to systemic purulent toxic-invasive disease manifestations [Fiedler T et al., 2015]. Simultaneously with the study of S.salivarius 24SMBc, my PhD project focalized on global regulation of virulence expression genes of three …
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Exploring the Polyclonal Antibody Responses Induced by Klebsiella pneumoniae Derived Outer-Membrane Vesicles
… five sets of polyclonal serum using outer-membrane vesicles (OMVs) before characterising the resulting antibody response. Each set of polyclonal serum was then screened against a curated collection of K. pneumoniae isolates (the Warne collection) before examining various antibody effector …
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Lysine and methionine transport by bovine jejunal and ileal brush border membrane vesicles
Purified brush border and basolateral membranes were isolated from homogenized intestinal enterocytes of Holstein steers by divalent cation precipitation followed by differential and sucrose density gradient centrifugation. Membrane marker enzymes were used to determine the effectiveness of the …
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Preparation of brush border and basolateral membrane vesicles from bovine intestine for nutrient uptake studies
Brush border and basolateral membrane vesicles were isolated by subjecting homogenized mucosal cells from bovine small intestine to a divalent cation aggregation followed by a series of differential and density gradient centrifugations. Membrane marker enzyme assays were used to monitor the …
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Studies on the synergistic protective activity of cancer antigens associated to engineered bacterial Outer Membrane Vesicles
Introduction Bacterial Outer Membrane Vesicles (OMVs) are naturally produced by all Gram-negative bacteria and are emerging as an attractive vaccine platform. The exploitation of OMVs in cancer immunotherapy was tested by decorating them with cancer epitopes and by following their capacity to …
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Isolation of hog renal brush border membrane vesicles with application to the study of cadmium nephrotoxicity
… small intestine, consists of two distinct plasma membrane surfaces referred to as the brush border, or luminal surface, and the basal-lateral, or contraluminal surface. Once isolated, membrane vesicles formed from the kidney cell can serve as models for the study of heavy metal mephrotoxin …
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Brucella abortus Strain RB51 Outer Membrane Vesicles as a Vaccine Against Brucellosis in a Murine Model
… vaccine to prevent human brucellosis. Outer membrane vesicles have been tested as potential vaccines to prevent diseases caused by bacterial species. OMV are constantly released from Gram-negative bacteria. They are comprised principally of the outer membrane components and periplasmic …
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The action of colocins E1 and K on proline transport in isolated membrane vesicles of E. coli.
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 1971
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Influence of osmotic forces and transbilayer membrane area imbalances on the stability and morphology of model membrane vesicles
… in many diverse biological functions including membrane fusion, maintenance of cellular shape and membrane trafficking. The osmotic stability of these large unilamellar vesicles (LUVs) in the present of plasma has also been investigated. This is of practical interest as these vesicles are …
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Exploring Host-Pathogen Interactions: Tracking SARS-CoV-2 evolution and assessing immune modulation exerted by Staphylococcus aureus membrane vesicles
L'evoluzione rapida dei virus a RNA e il ruolo delle vescicole di membrana batteriche (MVs) nelle interazioni ospite-patogeno rappresentano ambiti di ricerca cruciali nelle malattie infettive. Questo studio combina due indagini distinte: l'evoluzione di SARS-CoV-2 e l'impatto immunologico delle MVs …
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A Study of the Electrical Properties of Lipid Bilayers Modified by the Incorporation of Membrane Vesicles From Frog Ventricle
Made available in DSpace on 2014-12-14T05:40:43Z (GMT). No. of bitstreams: 1 7804094.pdf: 6047196 bytes, checksum: 857c10eac2b1e53642bdcc642850622b (MD5) Previous issue date: 1977
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