Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 253 for “"Membrane potential"”.
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Influence of mitochondrial membrane potential on the cryopreservation survival of hepatocytes
… agents and optimizing cooling rates. Membrane-permeable cryoprotectants, such as dimethyl sulfoxide, though widely used, can be extremely toxic to the cell. More natural, non-membrane-permeable cryoprotectants, inspired by freeze-tolerant animals have also been used. A …
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The role of membrane potential dynamics in cell behaviours : investigating the membrane potential dynamics in the Jurkat and HMEC-1 cell lines using the continuous wavelet transform
The role of the plasma membrane potential is most commonly associated with the generation of action potentials in excitable cells, however, experimental evidence suggests that this membrane potential is also linked to various behaviours in all cells (Blackiston et al., 2009). These cell behaviours …
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The stochastic modelling of the neuronal membrane potential in response to synaptic input
… the axon of the donor cell and the part of the membrane of the target cell onto which the axon impinges . The arrival of an impulse at the site of the synapse causes the release of a chemical neurotransmitter which then diffuses across a narrow gap and binds onto the receptors of the …
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Preparation for Nerve Membrane Potential Readings of a Leech, Laboratory Setup and Dissection Process
… setup, leech preparation process, and bio-potential data recording process are needed. Repeatability and quality data recordings are essential and thus dictate the requirements of the laboratory setup and processes listed above. Advances in technology have both helped and hindered this …
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Regulation of Intracellular pH and Plasma Membrane Potential in Trypanosoma Cruzi and Trypanosoma Brucei
… of intracellular pH (pHi) and the plasma membrane potential (DeltaPsi) was investigate in all stages of the pathogenic protozoans, T. cruzi and T. brucei utilizing the pH sensitive fluorescent dye BCECF and the potentiometric fluorescent dye bisoxonol. pH i in T. cruzi was found to be …
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Developing methods for measuring mitochondrial redox status and membrane potential in the isolated perfused heart
… redox and oxygenation status and mitochondrial membrane potential (ΔΨ<sub>m</sub>) respond to ischaemia-reperfusion due to a lack of suitable methods for measuring these bio parameters in *ex vivo* or *in vivo* models in near real-time. Current methods for measuring ΔΨ<sub>m</sub> rely on the …
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Development of Nanodiamond and Raman Spectroscopy Sensing Methods towards the Measurement of Mitochondrial Membrane Potential in Single Cells
Mitochondrial membrane potential ($\Delta\Psi_{\textrm{m}}$) in cells is a critical biological parameter that is related to diseases such as cancer through biological processes including metabolism, oxidative stress and apoptosis. Studying $\Delta\Psi_{\textrm{m}}$ in normal and diseased states is …
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Sustained Depolarization of the Resting Membrane Potential Regulates Muscle Progenitor Cell Growth and Maintains Stem Cell Identity in vitro
… that influence cellular properties. The resting membrane potential (Vmem) has proven to be a key parameter involved in cell proliferation, migration and differentiation. This current work is focused on elucidating the impact of sustained depolarization on MPC growth and differentiation in vitro. …
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Metaboloptics: In Vivo Optical Imaging to Enable Simultaneous Measurement of Glucose Uptake, Mitochondrial Membrane Potential, and Vascular Features in Cancer
… by providing information about tumor metastatic potential. Metabolism can further be used to guide treatment selection and monitoring, as cancer treatments can influence metabolism directly by targeting a specific metabolic dysfunction or indirectly by altering upstream signaling pathways. …
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Sign-Inverting Vectors Underlie a Coordinate Transformation in the Drosophila Central Brain
… is signaled in the canonical way, by raising the membrane potential and thus the spike rate of the PFNa neurons whose activity is aligned with a master heading signal. Wind from the back, however, is signaled with hyperpolarization of the PFNa neurons, which induces a non-canonical, prominent, 2-6 …
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5 ns Electric pulses evoke longer-lived calcium responses in adrenal chromaffin cells than the physiological stimulus: an experimental and computational study
… through VGCCs, and a disruption of mitochondrial membrane potential has been shown to delay calcium clearance, our first aim was to explore whether 5 ns pulses affect mitochondrial membrane potential using fluorescent mitochondrial membrane potential dyes. Control experiments established that …
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Modeling Action Potential Propagation During Hypertrophic Cardiomyopathy Through a Three-Dimensional Computational Model
… of that tissue and recording the resulting membrane potential with respect to time. The results of this analysis demonstrated that the developed model is able to depict variations in tissue thickness through the difference in membrane potential recordings. A one-way ANOVA analysis confirmed …
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ELECTROPHYSIOLOGICAL STUDIES ON ULMUS AMERICANA
<p>Although membrane potentials have been measured in algal cells and in large cells of some higher plants, this investigation is the first to include such measurements in mesophyll cells of elm (Ulmus americana) leaves. The inquiry entailed the development of the technique for measuring elm leaf …
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Magnetic resonance contrast-enhancing agents whose effects are altered by electric fields
The membrane potential is an important property of many cells and organelles. Changes in the potential of membranes control or accompany numerous biological processes including information transfer in neuronal networks. Magnetic resonance imaging (macroscopic and microscopic) is essentially a …
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Transcription Regulates Biased Mitochondrial DNA Inheritance
… the preferred method for creating mitochondrial membrane potential, which is important for the import of nuclearly encoded proteins into the mitochondrion as well as supporting oxidative phosphorylation. Loss of mtDNA causes problems with maintaining membrane potential and mitochondrial ATP …
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Activity-dependent integration and plasticity of new neurons during postnatal neurogenesis
… survival critically depends on the level of membrane depolarization. Once neurons integrate and survive in the brain circuits, neurons have the capability of monitoring their activity level and adaptively maintain their membrane excitability within the operational range. How they achieve the …
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Early hippocampal dysfunction in a mouse model of dementia with Lewy bodies: Mechanisms of cellular dysfunction caused by alpha-synuclein accumulation
… in mitochondrial number, dynamics, mitochondrial membrane potential and reactive oxygen species (ROS) production. The CamS120 mouse model was complemented with an alpha-synuclein overexpressing cell line (aFL), which was used to examine further changes in mitochondrial function, including changes …
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Towards unravelling the mechanism and function of exoelectrogenesis in cyanobacteria
… studies. During this screening, the Nernstian membrane potential reporter Thioflavin T (ThT) is identified as a compatible and favourable probe for use in this system. In recent years, membrane potential reporters (including ThT) have been used to inform on many aspects of microbial community …
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Optical method of recording electrical activity in isolated rabbit hearts
… optical technique generates recordings of transmembrane potential from excitable myocardial tissue, and remain free from stimulus artifacts that accompany electro stimulation and hinder all standard electrode recording methods during the application of high-voltage electrical shocks. The fiber …
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Novel Synthetic Compound, KR-22332, has radio-protective effect through inhibition of Mitochondrial Dysfunction and ROS Generation
… oxygen species generation, and mitochondrial membrane potential as well as changes in the signal pathway related to apoptosis were investigated. Then, the embryotoxicity of KR-22332 were explored in a zebrafish model. Radiation-induced apoptosis and altered mitochondrial membrane potential in …
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