Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Membrane penetration"”.
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Modeling the reaction mechanism of membrane penetration by striated amphiphitic gold nanoparticles
… results indicate that endocytosis and membrane poration could be ruled as possible mechanisms. In this work, we explore the reaction mechanism of membrane penetration using a coarse-grained Brownian Dynamics model. We also define a Monte Carlo simulation for modeling ligand motion on …
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Lysin Based Antimicrobial Peptides Against Acinetobacter Baumannii
… domain with a likely role in promoting outer membrane penetration. In the current study, we show that the C-terminal amino acids 108-138 of phage lysin PlyF307, named P307, alone was sufficient to kill A. baumannii (>3-logs). Furthermore, P307 could be engineered for improved activity, the …
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Cationic Polymers and Polymeric Micelles as Plasmid DNA and CRISPR-Cas9 Ribonucleoprotein Delivery Vehicles
… with liver-targeting ability and cellular membrane-penetration property have been developed and studied for their gene delivery efficiency and cytotoxicity.
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Tumor-penetrating delivery of small interfering RNA therapeutics
… ability of tumor-penetrating peptides with membrane penetration properties of protein transduction domains to direct siRNA to tumors in vivo. Second, we utilized this delivery system to bridge the gap between cancer genomic discovery and in vivo target validation. Comprehensive analysis of …
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… and the SNARE complex reconstituted in membranes in the presence of Ca2+, forming a quaternary SSCAP complex, and that the membrane penetration of synaptotagmin 1 into phospholipids is independent of the reconstituted SNARE complex. We also showed that synaptotagmin 1 displaces complexin …
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Cationic Glycopolymers for DNA Delivery: Cellular Internalization Mechanisms and Biological Characterization
… a smaller degree via macropinocytosis and direct membrane penetration. Caveolae-mediated, but not clathrin-mediated, internalization leads to transgene expression, suggesting a targeting opportunity based on uptake mechanisms. PEGylation of PGAA polyplexes was achieved to minimize polyplex …
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Chemical investigations into the physiology of bile acid skeletons
… of bile acids’ ability to traverse cellular membranes was undertaken using radioactively labeled of bile acids. A new methodology for ¿¿,¿¿-unsaturation of the side chain of bile acids was developed and the mechanism of the key step, dehydrogenation by selenium dioxide, was thoroughly …
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Cationic, α-helical polypeptides for cell penetration and non-viral gene delivery
… gene delivery vectors to achieve excellent cell membrane penetration and gene delivery. Firstly, I modified the complexes from the development of the supramolecular structures via incorporating a sugar containing polypeptide to form the ternary complexes. The ternary complexes contain the …
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Investigation into the lipid activation of calpain and its role in cataract formation
… a segment with the potential to form a lipid / membrane interactive a-helix (DWIH values circa 7.0). Extended hydrophobic moment analysis shows these segments to be potential oblique orientated a-helix formers (< uH > circa 0.5). This potential is confirmed by hydropathy plot and graphical …
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Targeting and Reprogramming Tumor-Associated Macrophages with Polymeric Nanoparticle-Mediated mRNA Delivery for Cancer Treatment
… endow nanoparticles with biocompatibility and membrane penetration ability, facilitating the entry of genetic material into cells. The Layer-by-Layer (LBL) strategy offers an effective method of encasing mRNA in nanoparticles. The principle of this strategy is to assemble layers of oppositely …
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Investigation into the structure function relationship of the membrane interaction of amphiphilic alpha helical antimicrobial peptides
Many eukaryotic organisms produce membrane interactive, a-helical antimicrobial peptides (a-AMPs) and a database of such peptides, together with selected physiochemical parameters was established. This database was divided into four groups according to the a-AMPs target organism(s) (active against …
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EMISSIVE PEPTIDE NUCLEIC ACIDS (PNAS): POTENTIAL TOOLS FOR INNOVATIVE DIAGNOSTICS AND THERAPEUTICS
… in clinical applications due to their poor cell membrane penetration and water solubility. Therefore, the research in the field of PNAs is still ongoing to improve PNA delivery and fully exploit their potential especially for therapeutic purposes. This Ph.D. thesis aimed to broaden the …