Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Membrane isolation"”.
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Nitrate carrier induction and characterization in maize root plasma membrane using chlorate-36 as a radiotracer analog for nitrate
… a change in nitrate carrier activity at the membrane level or an effect of nitrate metabolism has not been determined as yet due to the complexity of investigating nitrate transport with intact tissue. The use of a membrane vesicle approach has also been limited by the time associated with …
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Developing a Novel Cell Surface RNA Detecting and Profiling Method via RNA Metabolic Labeling
… to detect, isolate, and confidently characterize membrane-bound csRNA. Previously, csRNA has been profiled using methods based on cell membrane isolation that are expensive, laborious, and with unsatisfactory specificity and sensitivity . In this study, we use metabolic labeling and chemical …
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Characterisation of the Helix pomatia agglutinin binding glycoproteins of colorectal cancer cell lines and tissue samples
… cancer cells. A proteomic approach based on cell membrane isolation, pre-fractionation using lectin affinity chromatography, followed by 2-dimensional electrophoresis and MALDI-TOF-MS enabled the identification of the HPA binding proteins in the metastatic cancer cell line HT29. The proteins that …
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Isolation and characterization of dengue virus membrane-associated replication complexes from Aedes aegypti
… have long observed proliferation of host membranes. Membrane-bound replication compartments have recently been isolated and characterized from flavivirus-infected mammalian cells, providing insight into the morphology, organelle of origin, and protein components of the flavivirus …
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Proteomic Analysis of Plasma Membrane Proteins from Drug Susceptible and Drug Resistant Breast Cancer Cell Lines
… and pharmaceutical industries. Plasma membranes are rich in drug targets and other proteins responsible for cell signaling, transport, signal transduction, and other cellular functions. Information obtained about these proteins, and the pathways they participate in, helps to facilitate …
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Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2
… was characterized as a putative multi-pass transmembrane domain predicted to facilitate nsp2 packaging through association with the viral envelope. Within an in vitro cell-free translation system nsp2 was found to strongly associate with canine microsomal membranes. Through high-speed …
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Identification and characterization of diatom kinases catalyzing the phosphorylation of biomineral forming proteins
… for subcellular fraction of T. pseudonana membranes in combination with antibody inhibition assay, it was discovered that native tpSTK1 phosphorylates silaffins but not silacidins in vitro (i.e. it exhibits the same substrate specificity as recombinant tpSTK1). As tpSTK1 is an abundant …