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Showing 1 to 20 of 115 for “"Membrane fusion"”.
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Role Of Calcium In Membrane Fusion
… focused on understanding the role of calcium in membrane fusion at the atomic level. Membrane fusion is an intense area of experimental research,</p> <p>however, direct imaging of the membrane fusion mechanism has not been possible due to transient nature of the</p> <p>fusion process. As an …
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Cryo-electron Microscopy of Reconstituted Membrane Fusion Systems
… (SNARE) proteins are responsible for driving the fusion of biological membranes and are present across many different cell trafficiking systems. Synaptic SNARE proteins allow the release of neurotransmitters across synaptic clefts by promoting the fusion of vesicle membranes with neuron plasma …
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Light Scattering Measurement of Phospholipid Microvesicle Membrane Fusion Kinetics
… system which appears to evolve primarily by membrane fusion. Detailed kinetic characterization of the evolution by means of light scattering measurements appears to be practical.
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Luminal calcium regulates membrane fusion in the early secretory pathway
… trigger or is required at a basal level for many membrane fusion events. The role of calcium, and in particular luminal calcium, in regulating fusion in the early secretory pathway has not been thoroughly investigated. Here, I show that calcium has an inhibitory effect on homotypic coat protein …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… trigger or is required at a basal level for many membrane fusion events. The role of calcium, and in particular luminal calcium, in regulating fusion in the early secretory pathway has not been thoroughly investigated. Here, I show that calcium has an inhibitory effect on homotypic coat protein …
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How Atomic Level Interactions Drive Membrane Fusion: Insights From Molecular Dynamics Simulations
… on identifying the role of key players in the membrane fusion process at the atomic level with the use of molecular dynamics simulations. Membrane fusion of apposed bilayers is one of the most fundamental and frequently occurring biological phenomena in living organisms. It is an essential step …
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Polyphosphoinositide-derived signals in the regulation of vacuole membrane fusion and fission
The endo-membrane system of eukaryotic cells is a dynamic network of membrane-bound organelles that exists in a constant state of flux as they exchange membrane and protein cargoes. The yeast vacuole is a highly dynamic organelle that changes shape in response to both hypo and hyper-osmotic …
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The regulation of yeast homotypic membrane fusion by class C ABC transporters
… of eukaryotic cellular homeostasis requires the fusion of vesicle membranes that is accomplished by a SNARE-mediated mechanism. Membrane fusion is the merger of two lipid bilayers into one continuous membrane. Multiprotein complexes that have been conserved in eukaryotes carry out the basic …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
This is a comprehensive study of protein-mediated membrane fusion through single-molecule fluorescence resonance energy transfer (smFRET). Membrane fusion is one of the important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one …
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Regulation of membrane fusion by Tlg2p & Vps45p through the endosomal system of Saccharomyces cerevisiae
… components of the machinery that facilitates membrane fusion in eukaryotic cells. SNARE proteins are subject to multiple levels of regulation, one of which is imbedded in the syntaxin (Qa-SNARE) molecule. It has been demonstrated that some syntaxins adopt a closed conformation, whereby an …
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The protein structures underlying receptor binding and membrane fusion of ecotropic murine leukemia viruses
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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HIV entry : a biophysical and mutational analysis of gp41-mediated membrane fusion and its inhibition
… extensive conformational changes to mediate fusion of viral and cellular membranes. First, a short hydrophobic stretch of residues at the gp4 1 N-terminus insert into the target membrane, anchoring the protein in both viral and cellular membranes. This 'prehairpin' intermediate structure …
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Molecular Basis of Coupling Calcium Sensing to Fast Membrane Fusion by Synaptotagmin-1 in Neurotransmitter Release
The content of this work is based on the previously published articles that are cited below.
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Lipid composition and membrane physical parameters regulate priming and SNARE function in yeast vacuole membrane fusion
Fusion at highly specialized membrane microdomains is a prerequisite for processes like hormone and neurotransmitter release, antigen presentation and viral infection. Apart from a family of highly conserved proteins called SNAREs, membrane fusion also requires a set of regulatory lipids that …
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Coordination among proteins, lipids and water in membrane fusion and fission probed by solid-state NMR
… particle will pinch off a piece of the host cell membrane. The two membranes must be physically separated to release the virus particle. This process is called “scission” in the context of viral exit and “fission” when simply discussing membrane division. Viral membrane remodeling proteins …
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Inhibiting HIV-1 entry : utilizing a transient intermediate of viral membrane fusion as a target for drug discovery
… cells. The first step of this process is the fusion of viral and cellular membranes, which allows the release of the viral contents into the cell. An envelope glycoprotein on the surface of the virus is responsible for fusion. It is usually composed of two subunits, a surface subunit that …
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Contribution of the Membrane-Proximal Region of the Vesicular Stomatitis Virus Glycoprotein to Host Cell Entry and Membrane Fusion
… that the highly conserved residues in the membrane-proximal region of VSV G protein are critical to membrane fusion through participation in low pH-induced conformational changes and stability of theses structures. This hypothesis was tested initially through the use of site directed …
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Identification and Characterization of a Calmodulin -Binding Protein Related to a Family of ATPases Involved in Cell Division and Membrane Fusion
… including proteins involved in cell cycle and membrane fusion. A truncated fusion protein encoding the carboxy-terminal 22-amino acids of CIP111 was produced in E. coli and shown to bind CaM in a Ca2+-dependent manner by affinity chromatography. RT-PCR analyses demonstrated that CIP111 mRNA is …
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FMF assay for assessing vaccine generated antibodies in a biomimetic manner
… of influenza hemagglutinin responsible for virus membrane fusion in the endosomes. In order to study Influenza vaccine response in a more biomimetic manner and overcome the deficiencies of the traditional functional assays, we developed a fluorescent membrane fusion assay (fMF). The assay uses …
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An evolutionarily conserved regulatory mechanism for endosomal membrane trafficking
Membrane fusion in all eukaryotic cells is facilitated by the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes; a process that is regulated by Sec1p/Munc18 (SM) proteins. Membrane fusion has been conserved through evolution and hence a lot or our …
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