Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Membrane Translocation"”.
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Unconventional Secretory Pathways : Protein Folding and Quality Control During FGF2 Membrane Translocation
Fibroblast Growth Factor 2 (FGF2) ist ein proangiogener Wachstumsfaktor, der eine entscheidende Rolle bei der Tumor-induzierten Angiogenese spielt. FGF2 wird über einen bisher nicht bekannten Mechanismus von Säugetierzellen sezerniert, der auch nach Blockierung des ER/Golgi Systems durch Brefeldin …
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The Role of K63-Linked Ubiquitination Cycles In Akt Kinase Activation
… of Akt to phospholipid PIP3 on the plasma membrane and subsequent phosphorylation of Akt by its kinases. Growth factor-mediated membrane recruitment of Akt is a crucial step for Akt activation. However, the mechanism of Akt membrane translocation is unclear. Protein ubiquitination is a …
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Artificial Transmembrane Signalling
Cell membranes coordinate a large variety of biological processes by selectively recognising and responding to different external stimuli, and membrane-spanning proteins play a vital role in these signalling pathways. A large number of examples of synthetic membrane channels and transporters have …
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Protein mimetic nanoparticles
… a non-endocytic mechanism that generates minimal membrane perturbation. The membrane translocation capability of these nanoparticles makes them attractive candidates for engineering clinical applications that operate on a single-cell resolution. In particular, the physiochemical similarity between …
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Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites
… anchored in the outer leaflet of the plasma membrane mediated by myristate and palmitate residues covalently attached to the N-terminal SH4 domain of HASPB (Denny et al., 2000; Stegmayer et al., 2005). HASPB targeting to the plasma membrane depends on SH4 acylation, which occurs at …
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Folding, dynamics and interaction studies of the Neuropeptide Y family.
… as folding and the interaction with cellular membranes may greatly advance the treatment of human disease. The Neuropeptide Y family of peptides are highly conserved peptides found in nature, consist of 36 amino acids, and are widely distributed in the central nervous system of mammals. A …
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Targeting Nanoparticles to Tumor Vasculature
… were also used as probes for describing the membrane localization and exposure dynamics of PS and PE on the surface of irradiated cells. I have shown that PS and PE follow a similar exposure time course and they colocalize on the cell surface. PS and PE positive membrane patches appear to …
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Toxin-membrane interactions of Pasteurella multocida toxin and homologous domains
Toxin-membrane interactions are poorly understood on the molecular level due to the inherent difficulty of crystallizing membrane protein complexes and the large size (>50 kDa) of most functional AB or MARTX protein toxins. Toxins can interact with membranes at three stages during intoxication: 1) …
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The 52 and 60 kD Ro/SS-A : antigens where are they? : do anti-Ro/SS-A autoantibodies cause cutaneous disease?
… fluorescence. I studied the putative membrane translocation of 52 kD and 60 kD Ro under conditions of stress (UV A or UVB with or without hormones, drugs, vitamins and heat shock). I could not identify translocation of the 52 or 60 kD antigens with purified antibodies, although some …
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Receptor mediated targeting of liposomes
… Ricin-B-chain, a lectin which mediates membrane translocation of the toxic ricin-A-chain, was examined for its applicability for targeting of liposomes to cells. This lectin was shown to promote the binding of liposomes to cells and to mediate the delivery of cytotoxic concentrations of …
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The Role of IRAK-1 in the Regulation of Free Radicals and Oxidative Stress during Endotoxemia
… IRAK-1 deletion reduced LPS-triggered p47phox membrane translocation, suppressed NOX-1 expression and protein levels as well as hampered Rac1 activation. On the other hand, IRAK-1 deletion sustained antioxidative enzyme activity and levels in IRAK-1-/- macrophages and fibroblasts. In terms of …
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Cationic, α-helical polypeptides for cell penetration and non-viral gene delivery
… gene delivery vectors to achieve excellent cell membrane penetration and gene delivery. Firstly, I modified the complexes from the development of the supramolecular structures via incorporating a sugar containing polypeptide to form the ternary complexes. The ternary complexes contain the …
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Using Xenopus laevis to study the mechanisms governing the early germline development and the formation of vertebrate body plan
… for synthetic activation of RTKs involves membrane tethering of dimerizer-RTK ICD fusions. Depending on the intrinsic signaling capacity, however, this approach could entail undesirable baseline signaling activity in the absence of stimulus, thereby diminishing the system's sensitivity. To …
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LEUCINE-RICH REPEAT KINASE 2 (LRRK2) AT THE CROSSROAD OF INSULIN SIGNALLING AND PARKINSON S DISEASE
… regulation of glucose transporter type 4 (GLUT4) membrane translocation in myoblasts, the progenitors of mature muscle cells. Similarly to -cells, its kinase activity is required for RAB8-mediated ciliogenesis and ciliary localization of the insulin receptor. Interestingly, inhibition of LRRK2 …
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The Translocase of the Outer Membrane of Mitochondria (TOM complex)
… multisubunit assembly in the mitochondrial outer membrane, mediates targeting and membrane translocation of virtually all nuclear-encoded mitochondrial preproteins analyzed so far. In the present study the mechanisms by which the TOM complex recognizes different precursor proteins and translocates …
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Artificial Transmembrane Signalling and Cooperativity in H-Bonded Networks
… areas, with a first project on artificial transmembrane signalling, and a second on cooperativity in hydrogen bonded networks. Cells rely on complex signal cascades where proteins are responsible for the transmission and amplification of chemical signals across membranes. While numerous examples …
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The Role of Rac1 in Synaptic Plasticity, Learning and Memory
… modified to produce the “smart mouse” results in membrane translocation and activation of Rac1. Moreover, our laboratory has observed that translocation and activation of Rac1 are associated with fear learning in the adult mice. The purpose of this study was to examine whether Rac1 is required for …
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Investigation of the intracellular trafficking pathways of the dermonecrotic toxin family
… that govern cellular entry, trafficking, and translocation of PMT remain unclear. Co-localization studies described herein revealed that while PMT shares an initial entry pathway with transferrin (Tfn) and cholera toxin (CT), the trafficking pathways of Tfn, CT, and PMT subsequently diverge, …
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Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
… of distinct lipid species creates a unique membrane signature for various cellular organelles as well as acts as a localization signal for multiple proteins. Proteins have evolved to contain specific lipid-binding modules crucial for mediating protein-lipid interaction. Numerous human …
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Electrogenetic pathways in cyanobacteria
… of electrochemical gradients across cellular membranes. The variety of strategies that organisms have evolved to harness the many environmental sources of energy is astounding and still not fully understood. Cyanobacteria, uniquely in the prokaryotic kingdom, can harvest solar energy to couple …
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