Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Membrane Proximal External Region"”.
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Proteins Inside Cells and On the Surface of Membranes: Developing Three Dimensional In-Cell NMR and Structural Characterization of a Trimeric Membrane Proximal External Region Construct from HIV-1 gp41
… understand the biophysical behavior of the membrane proximal external region (MPER) of the HIV-1 envelope protein, gp41. This region is membrane associated and an important target for HIV-1 vaccine development because it contains the epitopes for several of the broadly neutralizing …
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Evaluating the neutralizing antibody response to HIV-1 membrane proximal external regional; Implications for vaccine design
… crucial for developing an effective vaccine. The Membrane Proximal External Region (MPER) within the HIV- 1 gp41 envelope is a promising vaccine target. The MPER is highly conserved, functionally constrained, facilitates virus fusion and is targeted by broadly neutralizing monoclonal antibodies. …
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HIV prophylaxis: an essential role for T cells and adjuvants in recombinant mucosal Lactobacillus acidophilus vaccines
… of Lactobacillus acidophilus expressing HIV membrane proximal external region (MPER), a portion of HIV envelope that contains broadly neutralizing antibody binding sites. We evaluated MPER-only expressing strains along with strains expressing adjuvants (interleukin-1β or flagellin) to improve …
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Design and characterization of novel immunogens for AIDS vaccine development and evaluation of a sample inference method for NGS Illumina amplicon data
… revived interest in vaccine development. The membrane proximal external region (MPER) of gp41 and the CD4 binding site (CD4bs) of gp120 have become attractive targets for vaccine development because they contain highly conserved epitopes recognized by some of the broadest neutralizing …
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Solid-state NMR investigation of viral fusion glycoprotein 41 (gp41)
Protein-mediated membrane fusion is an integral part of numerous cellular processes and viral entry. The HIV envelope glycoprotein mediates viral entry into target cells by fusing the viral envelope with the cell membrane. This process requires large-scale and multi-step conformational changes of …
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Characterization of structure and dynamics of membrane proteins from solid-state NMR
… of biomolecules. ssNMR is capable of studying membrane proteins in near-native lipid bilayers and is thus preferred over other biophysical techniques for characterizing the structure and dynamics of membrane proteins. This thesis primarily focuses on the study of the following membrane …
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Lipid-coated micro- and nanoparticles as a biomimetic vaccine delivery platform
… displaying minimal peptide epitopes from the Membrane-Proximal External Region of HIV gp41 (MPER) and enhancing their immunogenicity. Display within a lipid context was needed to provide the chemical environment appropriate for neutralizing antibodies, such as 4E10, to bind efficiently. …
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Enhancement of HIV vaccine efficacy via lipid nanoparticle-based adjuvants
… HIV antigen used was a peptide derived from the membrane proximal external region (MPER) of the gp41 protein. High-titer IgG responses to MPER required the presentation of MPER on liposomes and the inclusion of molecular adjuvants such as monophosphoryl lipid A. Anti-MPER humoral responses were …
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Model Membranes Study the Lipid-Reactivity of HIV-1 Antibodies and Vaccine Antigen
<p>One promising HIV-1 vaccine target is the membrane-proximal external region (MPER) of viral gp41. MPER is poorly immunogenic, however, the two rare neutralizing antibodies (NAbs), 2F5 and 4E10, bind to MPER with great neutralizing ability. Although their neutralizing mechanism represents a …
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The screening of neutralising antibodies against a resistant HIV-1 strain to identify novel epitopes
… 2G12 (anti-V3/glycan), 2F5 and 4E10 (both anti-membrane proximal external region). Of note, the virus is resistant to the extremely broad and potent mAb VRC01 (anti-CD4-bs). QH343.A10 was also found to be resistant to neutralisation by soluble CD4 (sCD4). This made the virus attractive to use in …
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Innate and humoral immune responses to HIV-1
… antibody 4E10. 4E10 recognizes an epitope in the membrane-proximal external region of the HIV envelope protein gp41. Previous attempts to elicit 4E10 by vaccination with envelope-derived or reverse-engineered immunogens have failed. It was presumed that the ontogeny of 4E10-equivalent responses …
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Role of envelope compactness and glycosylation in HIV-1 resistance to neutralising antibody responses
… resistant to commonly-elicited, anti-membrane proximal external region (MPER) antibodies in sera. To further understand its resistance, mutations in the MPER were introduced that are known to delay fusion following CD4-binding and thus increase the time the virus spends in the open …
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The Ontogeny of Vaccine-Induced HIV-1 Glycan-Reactive Antibodies
… the HIV-1 Env glycoprotein trimer including the membrane proximal external region, the gp120-gp41 interface, the CD4 binding site, V1V2-loop plus glycans, and the V3 loop plus glycans. Passive infusion studies with broadly neutralizing antibodies (bnAbs) of various specificities have been shown …