Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Membrane Microdomains"”.
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Regulation of Ras signaling and oncogenesis by plasma membrane microdomains
… study, we assessed the contributions of plasma membrane (PM) microdomain targeting to the functions of H-Ras and R-Ras. These paralogues have identical effector-binding regions, but variant C-terminal targeting domains (tDs) which are responsible for lateral microdomain distribution: activated …
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TORC2 and Eisosomes Are Spatially Interdependent, Requiring Optimal Level of Pi(4,5)P<sub>2</sub> for Their Integrity
… the organization and maintenance of the plasma membrane has been sought due to its numerous roles in cellular function. In the budding yeast Saccharomyces cerevisiae, a novel paradigm has begun to emerge in the understanding of the distribution of plasma membrane microdomains and how they are …
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Blood coagulation reactions on nanoscale membrane surfaces
… coagulation requires the assembly of several membrane-bound protein complexes composed of regulatory and catalytic subunits. The biomembranes involved in these reactions not only provide a platform for these procoagulant proteins, but can also affect their function. Increased exposure of …
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Costimulatory function of CD44
… relocation of the TCR/CD3 complex into the same membrane microdomains is required. The data are interpreted in the sense that the costimulatory function of CD44 relies on its cooperativity with the TCR. Most likely by recruitment of phosphokinases CD44 significantly lowers the threshold for the …
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Characterization of the Tetraspanin protein Tspan18
Tetraspanins are a superfamily of four-transmembrane proteins. They associate laterally with one another and with other transmembrane proteins to form membrane microdomains, in which associated proteins are regulated. Tetraspanins regulate diverse processes such as cell signalling and fusion, …
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STED microscopy in the visible range
… integral patch following fusion with the plasma membrane. Moreover, membrane microdomains were resolved with unprecedented (65 nm) spatial resolution. Furthermore, a novel doughnut-shape of the STED beam utilizing a helical phase ramp and a central singularity was established for STED. Finally, …
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The Role of Membrane Domains In Protein and Lipid Sorting During Endocytic Traffic
… lipid and protein composition of the plasma membrane (PM) must be tightly controlled to maintain cellular functionality, despite constant, rapid endocytosis. Because <em>de novo </em>synthesis of proteins and lipids is energetically costly, the cell depends on active recycling to return …
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Integration of signalling in smooth muscle caveolae
Caveolae and lipid rafts are plasma membrane microdomains that are considered to play a role in cellular signalling. Caveolins and cavins as well as cholesterol are required for the structure and function of caveolae. The papers summarized in this thesis examine the role of caveolae in smooth …
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Rotational motion and organization studies of cell membrane proteins
Cell membranes are dynamic structures with complex organization. The complexity of the cell membrane arises from intrinsic membrane structure, membrane microdomains within the plasma membrane and the membrane cytoskeleton. Plasma membrane receptors are integral membrane proteins with diverse …
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Effect of aging on metabolic status and hormone responsiveness in the ovary
… FBS and additional glucose. We then evaluated membrane lipid order to determine whether animal age affects the membrane structure of granulosa cells. Membrane lipid structure including formation of raft microdomains may affect signaling by receptors involved in reproductive and metabolic …
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Lipid Rafts in Pancreatic Beta- and Alpha-cell Stimulus-secretion Coupling
… examined. Cholesterol is the major component of membrane microdomains, termed lipid rafts. Numerous signaling and transport proteins have been found to be targeted to lipid raft microdomains, where the function of the associated membrane proteins could be distinctly regulated. I have identified …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… to the receptor binding subunit G1 and the transmembrane fusion subunit G2. Genetic studies were employed to understand the structure-function of the GP-C complex, with emphasis on elucidating the role of SSP in arenavirus life cycle. We present genetic evidence that support the inclusion of G2 …
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A Role For Lipid Rafts In Egfr Tki Resistance In Breast Cancer
… we have shown that EGFR localizes to discrete membrane microdomains (lipid rafts) in cell lines that are resistant to EGFR TKI-induced growth inhibition. Depletion of lipid rafts via cholesterol reduction results in sensitivity of EGFR TKI resistant breast cancer cell lines to the EGFR TKI …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… to the receptor binding subunit G1 and the transmembrane fusion subunit G2. Genetic studies were employed to understand the structure-function of the GP-C complex, with emphasis on elucidating the role of SSP in arenavirus life cycle. We present genetic evidence that support the inclusion of G2 …
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Correlation of Caveolin-1 Expression on Tissue Microarray with Prognosis in Renal Cell Carcinoma
… component of caveolae, which are plasma membrane microdomains implicated in the regulation of intracellular signaling pathways. Altered caveolin-1 expression is thought to be involved in several important human diseases including cancer. However, the role of caveolin-1 in renal cell …
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Visualization of cellular signaling by fluorescence resonance energy transfer in response to biochemical and mechanical microenvironments
… it reveals how Ca2+ signaling at the plasma membrane and ER membrane channels can be differently regulated by mechanical stimulation, in particular, distinct roles between active actomyosin contractility and passive cytoskeleton supports. In addition to these Ca2+ signals, I show that …
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Fluorescence resonance energy transfer-based subcellular visualization of FAK/Src activation by chemical and mechanical environment
… and FAK can be localized and activated at plasma membrane, which contains different microdomains called lipid rafts. In this thesis, specific Src/FAK biosensors based on fluorescence resonance energy transfer (FRET) were developed and further targeted to subcompartments of plasma membrane to …
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How to Mend a Broken Heart: Massive Endocytosis and the Role of Lipidic Forces in Membrane Trafficking
Novel forms of membrane internalization defined as massive endocytosis (MEND) were characterized for mechanism and physiological significance in isolated cells and intact cardiac tissue. These non-canonical forms of membrane trafficking are related to perturbations within the outer lipid monolayer …
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Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
… of cholera toxin B-labeled intracellular membranes to the plasma membrane. Cholera toxin-B labels the ganglioside GM1, which is enriched in lipid rafts. These specialized membrane domains are thought to serve as signaling hubs bearing many proteins that in turn, mediate trafficking steps …
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Implication of Bcl-2 family members in apoptosis signalling pathways triggered by growth factor deprivation
… analysis, the short isoform of the integral membrane protein (ITM2Bs) was identified in the present study as a novel regulator of cell death that was upregulated upon IL-2 deprivation. ITM2Bs was shown to belong to the BH3-only members of the Bcl-2 family and promoted apoptosis by …
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