Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 68 for “"Membrane Binding"”.
-
Membrane binding properties of Disabled-2
… is an adapter protein that interacts with cell membranes and it is involved in several biological processes including endocytosis and platelet aggregation. During endocytosis, the Dab2 phosphotyrosine-binding (PTB) domain mediates protein binding to phosphatidylinositol 4,5-bisphosphate (PIP2) …
-
Structural insights into the folding of cohesin and the membrane binding of bactofilin
… along chromosomes through an ATP-dependent DNA binding and release cycle. The cohesin complex is made up of Smc1, Smc3, Scc1 and additional subunits such as Scc3, Scc2, and Pds5. The Scc2 cohesin loader is involved in the “clamping” of DNA by the complex and promotes the hydrolysis of ATP …
-
The Ligand and Membrane-binding Behaviour of the Phosphatidylinositol Transfer Proteins (PITPa & PITPb)
… (PI) or phosphatidylcholine (PC) between membrane compartments in vitro. In Drosophila, the PITP domain is found to be part of a multi-domain protein named retinal degeneration B (RdgBα). The PITP domain of RdgBα shares 40 % sequence identity with PITPα and has been shown to possess PI and …
-
A Study of the Membrane Binding Properties of the Matrix Protein from Human Respiratory Syncytial Virus
… virus (RSV), and its interactions with model membranes composed of a range of lipids, which have been characterized using a variety of techniques. The M protein was expressed in E. coli with a histidine tag and purified by immobilised metal affinity chromatography (IMAP). The protein was found …
-
Use of Fluorescence to Study the Membrane Binding Domain of Cytochrome B(5) and the Induction of Membrane Domain Formation in Phospholipid Vesicles by Myelin Basic Protein
Embargo set by: Seth Robbins for item 86158 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs
-
MYO1C Binds to PiP2 With High Affinity Through a Putative Ph Domain
… that links the dynamic actin cytoskeleton to the membrane, and plays roles in mechano-signal transduction and membrane trafficking. We located and characterized two distinct membrane binding sites within the regulatory and tail domains of this myosin. We found that the tail domain binds tightly …
-
Designing Compounds that Probe the Phosphatidylinositol 3-Kinase Signalosome
… how ATP-competitive inhibitors affect membrane binding of PI3Kα; how known membrane binding inhibitors of coagulation factor V and VIII C2 domains affect PI3Kα- membrane interactions; and how compounds predicted to occupy a newly discovered drug binding site affect activity and …
-
Domain-based Bioinformatics Analysis and Molecular Insights for the Autoregulatory Mechanism of Phafin2
… PH and FYVE, contribute to Phafin2's cell membrane binding. Phafin2 also contains a poly aspartic acid (polyD) motif in its C-terminal region that can specifically autoinhibit the PH domain binding to membrane phosphatidylinositol 3-phosphate (PtdIns3P). Firstly, the study investigated the …
-
Protein-lipid interactions in molecular pathways of Parkinson’s and Alzheimer’s diseases
… and PD through aberrant interactions with lipid membranes. Despite current significant effort, the link between αS-/Aβ42-membrane interactions and disease mechanisms remains elusive. In this work, I applied a set of bulk and microfluidic assays to address this problem by quantifying misfolded αS- …
-
Regulation of proline utilization in Salmonella typhimurium: Autogenous regulation by a membrane-associated dehydrogenase
… and the putA gene, which encodes a bifunctional membrane-associated dehydrogenase that degrades proline to glutamate. The PutA protein also autogenously regulates transcription of the put operon. Induction of the put operon requires proline, oxygen (or another terminal electron acceptor), and …
-
Dissection of the Roles of Synaptotagmins in Calcium Dependent Neurotransmitter Release
… that still remain elusive. Syts have two calcium-binding C2 domains (C2A and C2B) which are crucial for their function. Clarifying how Syt C2 domains interact with the neuronal membrane fusion machinery and the membranes is essential for gaining insights into the mechanisms of calcium-triggered …
-
Mechanism of tocopherol transfer by human α-tocopherol transfer protein (α-hTTP)
… simply diffused from 6-His-a-hTTP to acceptor membranes since the kinetics of transfer were not responsive to a variety of conditions tested. After a series of trouble shooting experiments, we identified a minor contaminant, E coli. outer membrane porin F (OmpF) that co-purified with …
-
Computational investigation of membrane proteins across species and functional classes
Membrane proteins play central roles in cellular communication, metabolism, and homeostasis, yet experimental characterization of their dynamic interactions with membranes remains highly challenging. In this dissertation, I implement advanced molecular dynamics (MD) simulation workflows to …
-
Regulation and Functionality of Stress-Induced Cytochrome P450 Monooxygenases: A Functional Genomics Approach
Whereas Arabidopsis P450s are typically membrane-associated, the hydrophobic nature of membrane-binding regions oftentimes creates difficulties in the solubilization, isolation and study of these proteins using traditional biochemical techniques. Paralleling the study of individual P450s was the …
-
Studies of Protein Folding on Membranes and in Crowded Environments and Bridging the Research-Teaching Gap in K-12 Science
This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization …
-
Studies of protein folding on membranes and in crowded environments and bridging the research-teaching gap in K-12 science
This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization …
-
Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
… with phosphatidylinositol phosphates (PIPs) at membranes, and membrane curvature sensing to trigger actin polymerisation via the Arp2/3 complex. While membrane binding is key in in vitro purified reconstitutions of actin polymerisation, the lipid specificity and contribution of each membrane …
-
A Systems Biology Approach to Study Type III and Type IV Bacterial Effector Properties
… for their ability to associate with eukaryotic membranes. Unexpectedly, 30% of the virulence factor repertoire exhibited transmembrane-spanning domains, fatty-acid acceptor sites, peripheral membrane-binding properties, and/or cryptic phospholipid-targeting motifs. From a global analysis of …
-
Calcium -dependent protein kinases are myristoylated and associated with different membranes in Arabidopsis thaliana
… in many locations in plant cells, including the membrane fraction. However, little is known about the subcellular locations of individual CDPKs or the mechanisms involved in targeting them to those locations. Arabidopsis contains 34 genes that are predicted to encode CDPKs and 28 of the predicted …
-
Mechanistic Insights into the Role of Munc13 in Synaptic Vesicle Docking, Priming, and Fusion
… fusion of synaptic vesicles with the presynaptic membrane. These fusion events are tightly regulated by the influx of Ca2+, which is sensed by the complex protein machinery at the axon terminal. In order for these Ca2+-mediated fusion events to occur in the correct time and place, protein machines …
Page 1 of 4