Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Melanosomes"”.
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Identification of the TPC2 interactome reveals TSPAN10 and OCA7 as key players in the biogenesis of melanosomes
… to be repurposed for the differentiation of melanosomes from other endosomal compartments and specific delivery of melanosome synthesizing enzymes, Tyrosinase and Tyrosinase Related Proteins 1 and 2. Furthermore, correct melanosome maturation requires remodeling of the melanosome membrane, …
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The cellular basis of the Southern African forms of rufous & tyrosinase-positive oculocutaneous albinism
… ephelides, numerous fully melanised stage IV eumelanosomes, in addition to unmelanised stage I melanosomes, were seen in the melanocyte cytoplasm. Both stage IV and unmelanised stage I melanosomes are transferred to keratinocytes. In ephelis-free regions the melanocyte cytoplasm was filled with …
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New tools for the discovery of pigment gene function
… the MelanoIP method for rapidly isolating melanosomes and profiling their labile metabolite contents. We use it to study MFSD12, a transmembrane protein of unknown molecular function that when suppressed causes darker pigmentation in mice and humans. We find that MFSD12 is required to …
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Mechanisms and Regulation of Organelle Transport in Xenopus Melanophores
… to cyclically transport pigment granules, termed melanosomes, either to aggregate to the cell center or disperse throughout the cell in response to hormonal stimulus to effect color changes in the animal. We developed protocols for the biochemical purification of melanosomes from an immortalized …
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Signals Regulating Organelle Transport in Melanophores
… pigment. Furthermore, levels of myosin V on melanosomes are higher in cells dispersing pigment. Therefore the association of myosin V with melanosomes correlates with its dephosphorylation. Immunoprecipitation of cytoplasmic dynein showed that proteins at the molecular weights of the light …
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An ultrastructural and light microscopic study of melanocyte differentiation in chick embryos
… electron microscopy. The melanocytes contained melanosomes with a matrix of irregularly arranged filaments amongst typical eumelanogenic melanosomes. This suggests that these chick melanocytes may synthesize both eumelanosomes and pheomelanosomes in single cells. In a further study on pure …
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Role Of Type Ii Phosphatidylinositol 4-Kinases In Endosomal Tubule Dynamics During Melanosome Biogenesis
Melanosomes are pigment cell-specific lysosome-related organelles (LROs) in which melanin pigments are synthesized and stored. Melanosome maturation requires biosynthetic delivery of melanogenic enzymes (e.g. TYRP1), transporters (e.g. OCA2), and SNAREs (e.g. VAMP7) from early endosomes (EEs). One …
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Hypomelanosis in chickens
… melanocytes, however, they contained very few premelanosomes and fully melanised melanosomes were never observed, suggesting that hypomelanosis results from the arrested development (melanisation) of melanosomes in vivo. Two different experiments were carried out to determine whether this blockage …
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The Physical Basis of Extreme Animal Coloration
… all 16 species there was a continuous layer of melanosomes in the skin. My FDTD modeling showed that the melanosomes in the skin had the optimal size and shape to minimize reflectance. Furthermore, the melanosomes in deep-sea fishes are larger and more elongated for a given size than what is …
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Regulation of Bidirectional Microtubule-Dependent Organelle Transport in Xenopus Laevis Melanophores
… which contain pigment-filled organelles termed melanosomes that can be aggregated to the perinuclear region or dispersed throughout the cytoplasm by the motor proteins kinesin-2, cytoplasmic dynein, and myosin V. While the motor proteins responsible for melanosome transport have been identified, …
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Regulation of Myosin V-Cargo Interactions by Phosphorylation
… the ability of the motor to bind to Xenopus melanosomes. Similarly, myosin V recruitment to mouse phagosomes during phagocytosis is blocked by PAK1 phosphorylation. Thus, the binding of myosin V to cargo is regulated by phosphorylation of the tail domain, and may represent a common mechanism …
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Studying Processive Molecular Motors Inside Live Cells
… labeled peroxisomes and dark pigment carrying melanosomes can be tracked in vivo with high spatial (∼1.5 nm) and temporal (∼1 msec) resolution by the derivatives of FIONA. This has enabled us to resolve the stepwise motion of motor proteins conventional kinesin, cytoplasmic dynein, …
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Are 'exceptionally' preserved skeletal fossils necessarily exceptional chemically and cytologically?
… fossils. Durable cytological features such as melanosomes do appear to follow feather preservation, but traditionally exceptional fossils are not necessarily exceptional on a microscopic scale. Here, we analyze a feathered dinosaur specimen from the Jehol Lagerstätte to assess claims of blood …
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St John's Wort photomedicine for Melonoma
… co-localized with lysosomes and variably with melanosomes, the pigment producing organelles. No co-localization with the cell membrane, mitochondria, endoplasmic reticulum or nucleus was found. Investigating intracellular hypericin after treatment revealed a time-dependent decrease in all …
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Melanin as an Oto-Protective Pigment in Two Fish Species: <i> Poecilia Latipinna </i> and <i>Cyprinus Carpio </i>
… black P. <em>latipinna</em> morphs having larger melanosomes than white morphs. Hearing thresholds did not differ significantly among color morphs before sound exposure in either species. However, hearing thresholds post-sound exposure and the associated threshold shifts significantly differed …
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Transcriptomic and Proteomic Studies of Intercellular Communication Between Melanocytes and Keratinocytes in Human Skin
… produce melanin pigment which resides in melanosomes, organelles that are transferred to keratinocytes. In my thesis work, I focused on identifying downstream results of intercellular communication between melanocytes and keratinocytes for each of these cell types. I characterized the …
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The genomics behind beauty: disentangling the complex coat color genetic architecture of gir breed
… are responsible for melanin synthesis within melanosomes and is regulated by molecular networks that control the production of eumelanin and pheomelanin through specific signaling pathways. Within the context of population genomics, parameters such as linkage disequilibrium (LD) and effective …
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Biochemical Investigations of Macular Degeneration: The Significance of Protein Oxidation including Novel Methods for Its Study
… a complex granule exhibiting properties of both melanosomes and lipofuscin granules called melanolipofuscin (MLF). In contrast with the accumulation of LF in the RPE, MLF accumulation has been reported by Feeney-Burns to more closely reflect the onset of AMD. Although there have been significant …
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Development of High-speed Photoacoustic Imaging technology and Its Applications in Biomedical Research
… near-infrared wavelength of 1064 nm, where the melanosomes absorb more strongly than hemoglobin. Our high-speed PA flow cytography system successfully imaged melanoma CTCs in travelling trunk vessels. We also developed a concurrent laser therapy device, hardware-triggered by the CTC signal, to …
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Submicron-resolution Photoacoustic Microscopy of Endogenous Light-absorbing Biomolecules
… structures in vivo, such as red blood cells and melanosomes in melanoma cells. Compared with previous PAM systems, our high-resolution PAM could resolve capillaries in mouse ears more clearly. As an example application, we demonstrated intracellular temperature imaging, assisted by fluorescence …
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