Global ETD Search
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Showing 1 to 9 of 9 for “"Megaplasmid"”.
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus erythropolis AN12. These megaplasmids, pREA400, pREA250 and pREA100, migrate at approximately 400 kb, 250 kb and 100 kb, respectively. Genetic screening of an AN12 transposon insertion library showed that …
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Investigating the genome complexity of Streptomyces clavuligerus
… four linear plasmids pSCL1, pSCL2, pSCL3 and the megaplasmid pSCL4, which is the largest linear plasmid ever identified at 1.8 Mb in size. In order to study the genomic architecture of S. clavuligerus strains, we established an optimised method of pulsed-field gel electrophoresis, which allowed …
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Genomic diversity of Lactobacillus salivarius
… multireplicon genome architecture. The circular megaplasmids harboured by L. salivarius strains encode strain-specific traits for intestinal survival and probiotic activity. L. salivarius strains are increasingly being exploited for their probiotic properties in humans and animals. In terms of …
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Assembling improved gene annotations in Clostridium acetobutylicum with RNA sequencing
… basepair of prior studies. Curation of the pSOL1 megaplasmid reconciled transcriptome assembly statistics with findings from E. coli . The resulting annotation can be readily explored and downloaded through a customized genome browser, enabling future genomic and transcriptomic research in this …
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Mobilome and antibiotic resistance in Acinetobacter baumannii
… harbouring the blaPER-7, which was detected in a megaplasmid. The Mobilome, in the strains analysed, was composed of a wide variety of genetic elements, such as plasmids, insertion sequences, ISCR-like elements, which reflects the ability of A. baumannii to use different genetic platforms to …
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The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications
… this method, I cloned the large (178 kb) MP1 megaplasmid from D. radiodurans in E. coli. Finally, I developed a strategy to create seamless gene deletions in D. radiodurans which was demonstrated through the sequential genetic knockout of four restriction-modification systems. The tools and …
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Comparative microbial genome analysis of lactobacilli
… plasmids, followed by the repA-type circular megaplasmid, with the chromosome varying least of all. Glycosyl hydrolases, bacteriocins and proteases vary considerably on all replicons while two exopolysaccharide clusters show a lot of variation on the chromosome. In Chapter 4, I focussed on the …
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Entschlüsselung der Genome von <i>Ralstonia eutropha</i> H16 und <i>Methanosphaera stadtmanae</i> und vergleichende Untersuchungen zu Anpassungen der Genomorganisation
… Chr.2: 2,912 MBp) und dem bereits sequenzierten Megaplasmid pHG1 (452,156 Bp; Schwartz et al., 2003) zusammensetzt. Beide Chromosomen zeichnen sich durch ähnliche G+C-Gehalte (66.5 % und 66.3 %) und vergleichbare Anteile von kodierenden Sequenzen (87.8 % und 88.8 %) und Fremd-DNA aus (5 % und 13 …
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Defining genomic drivers of evolution in the entomopathogenic Serratia spp. A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… hypervirulent isolates reside on variants of a megaplasmid, pADAP (amber-disease associated plasmid), which encodes the Afp (antifeeding prophage) and Sep-Toxin complex. Pathogenic isolates of both species are found coexisting with non-pathogenic conspecifics (predicted to be around 44% of the …