Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Megakaryocytes"”.
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Molecular regulation of BRD3 in forward programming of Megakaryocytes
… Because platelets do not have nucleus, megakaryocytes are the best model to study chromatin interactors associated with platelet traits. I used CRISPR/Cas9 to generate a BRD3 KO iPSc model. These cells were capable to differentiate into MKs, using a forward programming protocol, …
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The role of megakaryocytes and platelets in infection and immunothrombosis
Megakaryocytes (MKs), one of the largest and rarest hematopoietic stem cells in the bone marrow, have traditionally played a primary role in hemostasis as precursors to platelets, which are importantly, one of the most abundant cell types in the peripheral circulation. While platelets are studied …
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Distinct functional effects of NMDA receptors in normal and leukaemic megakaryocytes
Human megakaryocytes were previously shown to release glutamate and express glutamategated Ca2+-permeable N-methyl-D-aspartate receptors (NMDARs). Their involvement in megakaryocytic maturation has been shown in 2003 but details remain limited. The importance of Ca2+ homeostasis in malignant …
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Infusing Factor Viii-Expressing Platelets Or Megakaryocytes As A Novel Therapeutic Strategy For Hemophilia A
… patients is ectopically expressing FVIII in megakaryocytes and platelets. This FVIII, termed pFVIII, is stored in alpha granules of platelets and is capable of restoring hemostasis in FVIIInull mice, even in the presence of circulating inhibitors. pFVIII has been proposed to be used for gene …
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Evaluation of the actin architecture in dysplastic megakaryocytes expressing the NUP98-HOXD13 leukemic fusion gene
Some myelodysplastic syndrome (MDS) patients present with macrothrombocytopenia due to impaired megakaryocyte (MK) differentiation. Transgenic mice that express the NUP98-HOXD13 (NHD13) fusion gene is a model for MDS and recapitulates the key features of MDS. The study investigated the hypothesis …
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Novel insights into megakaryopoiesis, thrombopoiesis and acute coronary thrombosis: transcriptome profiling of the haematopoietic stem cell, megakaryocyte and platelet
… of human haematopoietic stem cells (HSCs), megakaryocytes and platelets to gain insights into steady state and accelerated thrombopoiesis that occurs in states of haemostatic demand and in thrombosis by applying these findings to the pathological setting of acute coronary thrombosis. To …
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Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… the megakaryocyte lineage, to produce mature megakaryocytes, the platelet precursor cell type. A major limitation of the forward programming technique is a reliance of lentiviral transduction to overexpress the three TFs, which leads to a number of issues including heterogeneity and high …
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Targeting phosphatidylserine exposure in pro-coagulant platelets.
… These iPSCs were forward programmed to mature megakaryocytes where flippase activity could be assessed. ATP11A-/- and ATP11C-/- single knockouts had similar flippase activity to wild-type megakaryocytes, remaining sensitive to A23187 and NEM. ATP11A-/- ATP11C-/- double knockouts had inhibited …
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The pathogenesis of murine retroviral-induced noninflammatory spongiform polioencephalomyelopathy: Neuronal degeneration is indirectly mediated by resident microglial cells
… and consisted sequentially of: (1) splenic megakaryocytes, (2) splenic and thymic lymphocytes, (3) CNS capillary endothelial cells, and (4) CNS pericytes and microglia. Splenic megakaryocytes, platelets, and cell free viremia contributed to systemic dissemination of virus to the CNS. …
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Development and assessment of supercharged platelets as a novel drug delivery mechanism for promoting cardiac regeneration
… was employed for the genetic engineering of megakaryocytes, the precursor cells of platelets. Lentiviral plasmids, designed for the targeted delivery of proteins to the megakaryocyte and platelet α-granules, were modified to incorporate FGF2, VEGFA and TB4. Subsequently generated lentivirus …
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Evaluating the Diagnostic Value and Molecular Mechanisms of Thrombocytosis in Lung Cancer
… can directly induce proplatelet formation in megakaryocytes, the precursors to platelets. This effect was significantly greater in megakaryocytes exposed to lung cancer conditioned medium than those cultured in supplemented medium containing established drivers of megakaryopoiesis and …
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Functional Study of Hemogen Knockout Mouse Model
… and orthochromatic erythroblast cells and megakaryocytes, but absent in mature red blood cells and other differentiated blood cells. Hemgn knockout animals showed normal steady state and stress hematopoiesis and erythropoiesis. However, upon transplantation, a slight defect in lymphoid …
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Structure of the Human VPS33B/VPS16B Complex
… including the production by bone marrow megakaryocytes of protein-carrying alpha granules, which are absent in the platelets of ARC syndrome patients. Previous students in the Kahr Lab studied the VPS33B/VPS16B complex by a variety of means, including negative-staining electron microscopy …
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Engineering rFVIIa-loaded platelets; a novel approach to treating acute bleeding
… through endocytosis and genetic engineering of megakaryocytes differentiated in vitro from induced pluripotent stem cells (iPSCs). These are evaluated with in vitro assays to model the process of clot formation, as well as an in vivo model of haemostasis to compare their efficacy to conventional …
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An investigation of the structure and function of the receptor-type protein tyrosine phosphatase CD148
… of platelets exist, as has been noted for megakaryocytes. Flow cytometry (FC) experiments indicated that CD148 is uniformly expressed on all platelets; however, a population of CD148-positive unresponsive platelets was detected. My conclusions are that the ECD of CD148 shows a previously …
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Beyond the rare blood group Vel, uncovering the functions of SMIM1 in blood and in other organs
… datasets, I showed that SMIM1 is expressed in megakaryocytes (MKs), platelets, neutrophils and naïve, memory and class-switched B-lymphocytes. I further demonstrated that SMIM1 localisation and the type of multimers it forms in some of those cells are cell type dependent. Second, I assessed the …
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Regulation of the Gene Encoding Thrombin-Activable Fibrinolysis Inhibitor in Non-Hepatic Cells
… TAFI is originated from CPB2 gene expression in megakaryocytes. Moreover, our data imply a potential for site-specific anti-inflammatory control provided by macrophage-derived TAFI. Alternative splicing of the CPB2 mRNA may give rise to variants with an intracellular role, perhaps as a peptidase …
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Lipoids and blood platelets with reference to blood coagulation and the hemorrhagic diseases
… other formed elements such as thrombocytes, megakaryocytes, and stromatolytic erythrocytes 7) the multiplicity of factors which platelets may contribute to the blood clotting and hemostatic mechanisms 8) the occurrence of many clinical disorders due to deficiency of platelet functions. …
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