Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"Medical Biochemistry and Structural Biology"”.
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Investigating the nuclear import protein KPn߀1 as a cancer therapeutic target
… genes has been associated with the development and progression of cancer, many of which are being investigated as potential therapeutic targets. Previous work in our laboratory and others reported the elevated expression of the nuclear import protein Karyopherin Beta 1 (Kpnβ1) in various …
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Modulating the expression and activity of the nuclear import protein, Karyopherin β1, in cancer cells
… gene expression; the dysregulation of thousands of different genes has been associated with the progression of many types of cancer. Previous research from our laboratory aimed at identifying genes differentially expressed in cervical cancer compared to normal patient tissue, found …
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Monogenic hypercholesterolemia in South Africans : familial hypercholesterolemia in Indians and familial defective apolipoprotein B-100
LDL-receptor mutations and familial defective apolipoprotein B-100 (codon 3500) (FOB), the known causes of monogenic hypercholesterolemia (MH), have similar clinical features. The nature of the mutations responsible for MH in South Africans of Indian origin was previously unknown. Similarly, the …
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Biosynthesis and degradation of proteoglycans in cultured smooth muscle cells
… isolated from neonatal rat hearts synthesize and secrete radioactively labelled proteoglycans into two distinct extracellular compartments, the pericellular (cell surface/matrix layer) and the culture medium (extracellular). Cultures grown in the presence of ascorbic acid synthesize …
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Rat angiotensin-converting enzyme : tissue specific expression during pharmacological inhibition
… vasomotor tone via its vasoconstrictor action, and also increases salt and water retention by stimulating the release of aldosterone. ACE inhibitors, such as captopril, enalapril and lisinopril, are highly effective in the treatment of hypertension and congestive cardiac failure. Previous …
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Characterising the anticancer effects of a small molecule with potential to inhibit nuclear import via karyopherin beta1
… nuclear pore assembly, nuclear membrane assembly and DNA replication. Since all these cell regulatory functions are critical for normal cell function, dysregulation of Karyopherin proteins may have an impact on cancer cell survival. Previous research in our laboratory and in that of others has …
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The glutathione S-transferases : kinetics, binding and inhibition
… with a variety of electrophilic molecules, and they are therefore thought to play a major role in drug biotransformation and the detoxification of xenobiotics. The cytosolic GSH S-transferase isoenzymes of rat, man and mouse have been assigned to three groups, Alpha, Mu and Pi, based on …
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The role of seminal fluid in cervical squamous carcinoma progression: Impact on cell proliferation, EMT, motility and gene expression
… is the leading cause of cancer related deaths and the second most common cancer amongst South African women. The key cause for cervical cancer development is sexual transmission and persistent infection with high-risk Human Papillomavirus (HPV). However, it takes several years from infection to …
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Binding of Mycobacterium tuberculosis to complement receptor type 3 expressed in mammalian cells : dependence on serum opsonins
… receptors, of which the mannose receptor and the complement receptor type 3 (CR3) may support nonopsonic binding. CR3, a β₂ integrin, is a target for diverse intracellular pathogens, but its role in nonopsonic binding remains uncertain. We have examined the binding of M. tb. to human CR3 …
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Identification and characterisation of proteases in Mycobacterium tuberculosis
… within its host cell, the macrophage, and its ability to cause extensive tissue necrosis. Exported proteases are commonly associated with virulence in bacterial pathogens, yet their role in Mycobacterium tuberculosis has virtually not been studied. Preliminary experiments showed M. …
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The molecular basis of alpha thalassaemia in a South African population
… endonuclease digestion, Southern blotting and hybridisation with alpha and zeta globin-specific probes were used to investigate the incidence of the the various alpha thalassaemia determinants and their disorders. Results indicate that one determinant in this population results from the …
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Investigating excitatory GABAergic signalling & benzodiazepine resistance in an in vitro model of status epilepticus
… GABAergic signaling in an in vitro model of SE and how this may affect the anticonvulsant efficacy of the benzodiazepine, diazepam. Firstly, I confirm that benzodiazepine-resistant SE is prevalent in a South African paediatric population. Secondly, consistent with its established mechanism of …
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The effect of seminal fluid on TBx2 and TBX3 expression and activity in cervical cancer cells
… cancers in Africa, both in terms of incidence and mortality, and is disproportionately prevalent in developing nations due to a lack of adequate access to healthcare. While new vaccine technologies are rapidly reducing the incidence of Human Papilloma Virus (HPV) infection, the primary …
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The biological properties of three trichothecene mycotoxins produces by fusaris
… neosolaniol monoacetate, was isolated and purified from cultures of Fusarium sambucinum. Since little is known about its toxic properties, the biological effects of this trichothecene were compared to those caused by diacetoxy-scirpenol in male Wistar rats. The lesions caused by the two …
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Non-lysosomal protein degrading systems in chicken skeletal muscle
In an attempt to understand the roles played by the ubiquitin-dependent and calpain pathways in protein degradation in chicken skeletal muscles, biochemical studies were conducted on components of these two systems as well as their potential endogenous and exogenous substrates. ATP- and …
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Purification and some properties of an alkaline protease from rat skeletal muscle
… have been described by a number of researchers and have been purified to varying degrees. Such alkaline proteases may play an important role in the metabolism of myofibrillar and other muscle proteins and as such deserve to be fully characterised. In this study, a major myofibrillar alkaline …
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Natural product derivative activates autophagy in cancer cells
Artemisinin, a natural product and its derivatives are potent antimalarial compounds, which have shown anticancer activity. In this study, we further characterized a novel artemisinin derivative namely EXP57EA which was previously designed and synthesized by the Chemistry Department at the …
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Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes
… in particular, early endosome processing and delivery to the lysosomes. Two models for endosome processing have previously been proposed in the literature, namely the maturation and vesicular traffic models. The general consensus has been an early phase of intermingling of the endocytic …
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Protein degradation in rat skeletal muscle : intracellular, non-lysosomal enzyme systems and their endocrine control
… the work reported in this thesis were submitted and accepted for publication, the abstracts of which follow: SOLUBLE AND PARTICULATE FORMS OF MUSCLE ALKALINE PROTEINASE SHOW DIFFERENTIAL SENSITIVITY TO ENDOGENOUS INHIBITOR(S): Firhaad Ismail and Wieland Gevers, (Biochemistry International, In …
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