Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Mediator complex"”.
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Mechanisms of Transcriptional Control by the Mediator Complex Subunit Cdk8
… of non-protein-coding genes. The multi-subunit Mediator complex functions in RNAPII transcription regulation by acting as a bridge between RNAPII and transcription factors. Cyclin-dependent kinase 8 (Cdk8) is the only enzymatic subunit in the entire Mediator complex, and it resides in a …
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MED9, a Mediator Complex Component, and its Interacting Protein MORC1 Balance Growth and Defense in Arabidopsis
… to the same pathogen. MIP13 encodes MED9, a mediator complex component that interfaces with RNA polymerase II and transcriptional factors to reprogram transcriptional activities. Transcription dynamics assessments at multiple-time points of defense genes, up- and down-regulated, reacting to …
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Identifying Novel Interaction Between CDK8 Kinase Module and The Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… by the transcriptional co-activator, the Mediator complex. Our group has determined that Cyclin Dependent Kinase 8 (CDK8) and Mediator Complex Subunit 12 (MED12), components of the kinase module in the Mediator complex, contain consensus sequences for binding to the N-terminus of USP7. …
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Genome-wide analysis of transcription factors and chromatin in S. cerevisiae
The regulation of transcription initiation is a complex phenomenon, involving DNA-binding activator and repressor proteins, histones and chromatin factors, histone-modifying enzymes, and the general transcription apparatus. Though many of the genes encoding these factors have been identified in …
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Isolation and Characterization of Estrogen Receptor- Interacting Proteins
… hormone receptor associated protein (TRAP)/Mediator coactivator complex, which interacts with ERα and ERβ both in unfractionated nuclear extracts and in purified form equally efficiently. Although some of other interacting proteins displayed subtype specificity, the main focus of the work …
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Developing and Using Methyl-Specific Antibodies to Study The Biological Roles of Arginine Methylation
… novel cellular targets for CARM1, including Mediator Complex Subunit 12 (MED12) and the lysine methyltransferase KMT2D/MLL2. Both of these proteins are implicated in enhancer function. We identified the primary CARM1-mediated MED12 methylation site as arginine 1899. Using methyl-specific …
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Med12 Is A Critical Regulator of Neural Crest Lineage and Nervous System Myelination
<p>The Mediator complex (MED) is a multi-subunit protein complex integral to the eukaryotic transcription machinery. MED12 is a Cdk8- regulatory kinase module subunit directly implicated in human disease and is genetically altered in neurological disease and cancer. Numerous attempts at generating …
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Characterizing the Mechanisms Regulating Enhancer Commissioning by MyoD in Muscle Cells
… Our results reveal a link between MyoD and the Mediator complex, a key component of enhancer-promoter looping. Next, using phenotypic assays and transcriptomics, we demonstrate that knocking down Mediator function severely impedes muscle differentiation by down-regulating essential muscle genes. …
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Identification and functional characterization of the novel MAR-binding protein, SATB2
… enzymes or nucleosome-remodeling complexes, and the stimulation of RNA polymerase via an interaction with the mediator complex. In addition to enhancers and promoters, nuclear matrix attachment regions (MARs) have been implicated in the regulation of gene expression by altering the …
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Regulation Of Transcription By RNA Polymerase II In <i>S. pombe</i>
… with MLL in leukemia and forms a tight complex with ELL-associated factors (EAF). ELL/EAF is an RNA polymerase II elongation factor that in metazoa can assemble into a larger assembly that also includes P-TEFb and other proteins encoded by genes involved in MLL translocations. This …
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Identification and Characterization of the TGFβ/Hippo Crosstalk through Mathematical Modeling
… are intimately interconnected. Hippo pathway mediators Taz/Yap interact with the TGFβ regulated Smads and when Hippo is activated, the cytoplasmically localized Taz/Yap reduces or prevents Smad nuclear accumulation thereby dampening TGFβ induced transcriptional activity. In the nucleus, …
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From Flies to Mice: Drosophila as a Model System to Study Fat Biology
… by directly binding Med23, a component of the Mediator Complex, which connects transcription factors with RNA polymerase II. Taken together, I have shown that both the Hh and Adp pathway have evolutionary conserved functions in fat formation. These results support the idea that Drosophila is a …
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Proteomic characterisation of Estrogen Receptor (ERα) interactome in breast cancer
… for the assembly and chromatin binding of the Mediator complex and general transcription factors, whilst RNA-sequencing and proteomic analysis showed a decrease in expression of genes linked to cell cycle followed ZNF207 knockdown. The effect was likely mediated by changes in the chromatin …
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Biomolecular condensation of intrinsically disordered regions in P. falciparum and H. sapiens transcription factors
… especially in sub-Saharan Africa. The organism's complex life cycle is tightly coupled to a carefully controlled gene expression programme, and its highly divergent transcriptional machinery suggests unique mechanisms of transcriptional control. Biomolecular condensation, driven by intrinsically …
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Role of Phosphorylated Dicer1 In Tumor Progression
… a novel multimeric chromatin-DICER1 nuclear complex comprised of the Mediator complex subunit-12, CBX1, MACROH2A.1 and transcriptional regulators. Altogether, these findings support the model that phosphorylated nuclear DICER1 functions downstream of the KRAS/ERK axis to alter chromatin …
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Interpreting Notch signals: characterisation of dynamic transcription hubs by live imaging
… of how Notch activation results in transcription complex recruitment at gene regulatory regions and how this translates in the activation of target genes. A live imaging approach was taken to understand the mechanisms for robust activation of Notch targets, focusing on the co-activator Mastermind, …