Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 172 for “"Mechanotransduction"”.
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Mechanotransduction at cell-cell contacts
For nearly 40 years, it was debated as to whether the differences in cadherin- dependent cell-cell adhesion energies could solely predict cell sorting. Although adhesion energies do influence cell sorting in vitro, recent studies suggested that that differences in cell adhesion, determined mainly …
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Cadherin specificity in adhesion and mechanotransduction
… Besides, depending on cell context, the cadherin mechanotransduction is subtype-specific. To study how cells respond to passive substrate rigidity, MCF-7 cell spreading on soft gels coated with E-cadherins was examined. The spreading area of cells increases with substrate rigidity. This behavior …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
Living organisms are made up of a multitude of individual cells that are surrounded by biomolecules and fluids. It is well known that cells are highly regulated by biochemical signals; however it is now becoming clear that cells are also influenced by the mechanical forces and mechanical properties …
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E-cadherin mechanotransduction beyond cell-cell junctions
… signal transduction pathways in cadherin-based mechanotransduction. In this thesis, I investigated the role of E-cadherin in mechanotransduction, using modified Magnetic Twisting Cytometry (MTC) and Traction Force Microscopy (TFM) coupled with fluorescence imaging. A major focus of Chapter 2 is …
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Quantitative analysis of subcellular biomechanics and mechanotransduction
… and cytoskeletal remodeling, a process termed mechanotransduction. Intracellular mechanosensors are thought to be activated by conformational changes induced by local cellular deformations. Since these mechanosensors have been speculated to be located in several cellular domains including the …
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Developing platforms to investigate mechanotransduction during wound healing
… matrix assembly, and their role in regulating mechanotransduction to direct cell migration to dictate normal or fibrotic wound healing remains critically understudied. The isoform EDA-fibronectin contains an Extra Domain A insert that is only expressed in adult tissue during wound healing or …
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Molecular and Cellular Mechanisms of LIM Protein Mechanotransduction
… mechanical stimuli into biochemical signals (mechanotransduction) are poorly understood. The actin cytoskeleton serves as a nexus for cellular mechanotransduction, generating and transducing forces into intracellular signals that coordinate cell migration, cellular contractility, organelle …
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The Role of Wnt Signaling in Bone Mechanotransduction
… 4 (Lrp4), in the regulation of bone mass and mechanotransduction. I generated an Lrp4 knockin mouse model harboring a missense mutation found among human patients with abnormally high bone mass. I hypothesize that the mutation compromises sclerostin action on bone cells. Understanding how each …
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Pathways for mechanotransduction in pressurized airway epithelial cells
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2001.
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The mechanics of mechanotransduction : analyses of cell perturbation
… gene expression, motility. This process, termed mechanotransduction, was investigated using computational and theoretical approaches, as well as comparisons with experiments. As a first step, a three-dimensional viscoelastic finite element model was developed to simulate cell micromanipulation by …
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PIEZO1 Channel-Mediated Mechanotransduction as a Driver of Cardiac Remodelling
… to determine how PIEZO1-mediated fibroblast mechanotransduction contributes to hypertrophy and fibrosis under pressure overload, and whether pharmacological inhibition of PIEZO1 signalling could offer therapeutic potential. To address these aims, I generated fibroblast-specific Piezo1 …
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Osteocyte mechanotransduction: changes with age - a parametric finite element study
… of bone is essential to understanding the mechanotransduction within the osteocyte network. The purpose of this study was to analyze the effect of age on the microarchitecture of murine bone and use the data to conduct a finite element analysis of strains imposed on the lacuna. Ten murine …
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THE NON-ERYTHROID MEMBRANE SKELETON AND ITS ROLE IN MECHANOTRANSDUCTION
… mechanics, adhesion, traction and possibly mechanotransduction. In this work, I was able to determine the amount -down to the average copy number per cell- of the main protein components of the non-erythroid membrane skeleton, in model cell lines commonly used in cell-mechanics studies. The …
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Experimental and analytical techniques for studying mechanotransduction in articular cartilage
Tissues are often complex heterogeneous systems, where individual cells coordinate responses to internal and external stimuli. To fully understand cell function in such environments, it is critical to connect the behavior of individual cells with the full tissue-scale response. Articular cartilage …
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Life Under Tension: Computational Studies of Proteins Involved in Mechanotransduction
… that transform forces into biochemical signals (mechanotransduction). The first protein studied is the mechanosensitive channel of small conductance MscS. This membrane channel has been proposed to act as a safety valve during osmotic shock, facilitating the release of ions and small solutes upon …
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VE-cadherin mechanotransduction signaling and its regulation by extracellular matrix
… that increased tension on VE-cadherin triggers a mechanotransduction response that affects endothelial permeability. In this dissertation, I focus on VE-cadherin force-activated signaling cascades that mediate endothelial permeability. At the same time, cadherin-based mechanotransduction also …
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Fluorescence laser tracking microrheology for quantitative studies of cytoskeletal mechanotransduction
… and diversify the scientific knowledge on mechanotransduction, from a molecular and cellular standpoint.
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Mechanotransduction via airway epithelial cells : the effect of compressive stress
… to airway remodeling. To better understand this mechanotransduction mechanism we used an in vitro system of cultured normal human bronchial epithelial cells that could simulate compressive stresses experienced by the epithelium during bronchoconstriction. The application of a transcellular …
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Flow-induced mechanotransduction in cell-cell junctions of endothelial cells
Endothelial cells show an unexpected behavior shortly after the onset of laminar flow: their crawling speed decreases ~40% within the first 30 min, but only in a confluent monolayer of endothelial cells, not in subconfluent cultures, where cell-cell interactions are limited. This led us to study …
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Chondrocyte gene expression and intracellular signaling pathways in cartilage mechanotransduction
Chondrocytes respond to in vivo mechanical loads by regulating the composition of the cartilage extracellular matrix. This study utilized three loading protocols that span the range of forces and flows induced by in vivo loading. Constant (static) compression of cartilage explants induces a …
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