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Showing 1 to 20 of 43 for “"Matrix-assisted laser desorption/ionization (MALDI)"”.
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TARGET MODIFICATION FOR ENHANCED PERFORMANCE MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) MASS SPECTROMETRY
… TARGET MODIFICATION FOR ENHANCED PERFORMANCE MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) MASS SPECTROMETRY MAJOR PROFESSOR: Dr. Gary R Kinsel MALDI MS, a powerful tool for the analysis of biomolecules, has undergone major advancement in instrumentation to yield improvements in …
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DEVELOPMENT OF A NOVEL MATRIX ASSISTED LASER DESORPTION / IONIZATION (MALDI) BASED PEPTIDE QUANTITATION APPROACH
Matrix Assisted Laser Desorption / Ionization (MALDI) Mass Spectrometry (MS) has emerged as an important tool in the field of proteomics mainly because it is simple, quick and efficient. The identification and quantitation of biomarkers, protein targets for drugs, and metabolites are some of the …
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Optimization of sample preparation in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry of macromolecules
<p>New method for the matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) and Fourier transform mass spectrometry (MALDI-FTMS) analysis of low molecular weight polyvinyl acetate (PVAc) was developed and then applied to the characterization of commercially available chewing gum. …
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Identification and Characterization of Bacterial and Bacteriophage Proteins Via Matrix-Assisted Laser Desorption Ionization (Maldi) Mass Spectrometry
… have emerged. Here, it is shown how using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, coupled with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), one can identify and characterize bacterial and bacteriophage proteins.</p>
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Oligosaccharide Analysis via Anion Attachment Using Negative Mode Electrospray (ES) and Matrix Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
… of different acidities were investigated in MALDI mass spectrometry.
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MATRIX-ASSISTED LASER DESORPTION/IONIZATION (MALDI) TARGET MODIFICATION FOR ENHANCED PROTEOMICS ANALYSIS AND PLASMA POLYMER CHARACTERIZATION BY MALDI MASS SPECTROMETRY
… three surface modifications are used to produce MALDI targets having reduced surface-protein binding affinity with a goal of increasing peptide/protein MALDI ion signals and lowering the limits of detection (LODs) for proteins and peptides. The second section discusses a bioselective MALDI …
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Microseparation Strategies for the Assay of Neuronal Samples
… described here are the culmination of HPLC, matrix assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry, and molecular biology techniques to quantify and characterize the novel peptides.
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Membrane Processing of Ethanol -Extracted Corn Oil
… with field-emission gun (ESEM-FEG) and matrix-assisted laser desorption ionization (MALDI) mass spectroscopy. Economics of the dry grind process with the new membrane processes for oil recovery were shown to add considerable value.
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MALDI-TOF MS Data Processing Using Wavelets, Splines and Clustering Techniques.
<p>Mass Spectrometry, especially matrix assisted laser desorption/ionization (MALDI) time of flight (TOF), is emerging as a leading technique in the proteomics revolution. It can be used to find disease-related protein patterns in mixtures of proteins derived from easily obtained samples. In this …
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Detection and Imaging of Neurotransmitters in Rodent Brain Tissue Using FMP-10 and ZnO Nanoparticle Matrices with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is an analytical technique used to visualize the spatial distribution of molecules within biological samples. In our lab, we employ two complementary matrices, 2-fluoro-1-methyl pyridinium (FMP-10) and zinc oxide …
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Development of a novel electron-transfer secondary reaction matrix, characterization of the site–specificity of novel bilin-lyase, and Fundulus grandis protein expression investigation using mass spectrometry
… but a common thread is that each employs matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to solve a problem. Fundamental aspects of MALDI in-plume ionization are implicated in the introduction of a newly developed electron-transfer secondary ionization matrix. The …
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Development of High-Throughput Platforms for Single-Cell Analysis
… of tens of thousands of cells based on matrix assisted laser desorption/ionization (MALDI) mass spectrometry. We evaluated several materials and approaches to chip fabrication suitable for interfacing with a MALDI instrument. We also developed an analytical pipeline for efficient …
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Development Of Matrix Assisted Ionization Methods For Characterization Of Soluble And Insoluble Proteins From Native Environments By Mass Spectrometry
<p>Matrix-assisted laser/desorption ionization (MALDI) and electrospray ionization (ESI) have made a huge impact in the analysis of biological materials. ESI has gained its popularity involving liquid based analysis, efficient fragmentation, chromatographic and electrophoretic separations but has a …
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Distinguishing Macrophage Activation States by Mass Spectrometry
… are commonly prepared to be compatible with matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) experiments. The tested combinations of MALDI preparation techniques and instrument parameters however remained unsuccessful at distinguishing activation states. Through …
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Peak intensity prediction in mass spectra using machine learning methods
… diesem Zweck wird mithilfe von Datensätzen eines Matrix-Assisted Laser Desorption Ionization (MALDI)-Massenspektrometers ein Verfahren entwickelt, das Simulation und Überwachte Lernverfahren kombiniert. Der zweite Teil der Arbeit hat zum Ziel, jene Peptideigenschaften zu ermitteln, die den größten …
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Development Of Inlet And Vacuum Ionization Methods For Characterization Of Biological Materials By Mass Spectrometry
<p>Inlet ionization and vacuum ionization are novel ionization methods to produce electrospray ionization (ESI)-like ions from the solid or liquid states, operating from atmospheric pressure (AP) or vacuum, without the use of voltage or the necessity of high energy input such as a laser or particle …
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Gas-Phase Covalent And Non-Covalent Ion/ion Chemistry Of Biological Macromolecules
… base ion/ion chemistry has been demonstrated on matrix assisted laser desorption/ionization (MALDI)-derived peptide ions. Such Schiff base ion/ion chemistry, whether electrospray or MALDI-derived, can produce complementary or even an increase in structural information. Multiple covalent …
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Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
… (RP-HPLC), and characterization using matrix-assisted laser desorption/ionization (MALDI) mass spectroscopy. The synthesized conjugates with a diameter of internalization, thus falling in a relatively stable range of zeta potential values (13-18 mV at N/P 40). All peptide:siRNA …
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Microfluidic Interfaces for Mass Spectrometry: Methods and Applications
Since the introduction of electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI), there has been an unprecedented growth of biomolecule analysis using mass spectrometry (MS). One of the most popular applications for mass spectrometry is the field of proteomics, which …
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Laser ablation sample transfer for mass spectrometry
… spectrometry was developed that uses an infrared laser to ablate materials under ambient conditions that are captured in a solvent or on a surface. An infrared optical parametric oscillator (OPO) laser system at 3 μm wavelength was focused onto samples for ablation at atmospheric pressure. The …
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