Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 95 for “"Matrigel"”.
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Regulation der Invasion von Bronchialkarzinomzellen unterschiedlicher Differenzierung in die Basalmembran in Kokulturen und in Matrigel-Kammern
… Dazu wurden die Modelle der Kokultur und der Matrigel-Kammern verwendet und miteinander verglichen. Die Invasion von Tumorzellen durch die Basalmembran der Organkultur wurde in histologischen Präparaten analysiert. Die Durchwanderung der Zellen in Matrigel®-beschichteten Kammern und das …
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Cell-cell communication in three dimensional microenvironments
… diacrylate and the basement membrane matrix Matrigel were used. Optical tweezer technology allowed precision placement and formation of homotypic and heterotypic arrays of human U937, HEK 293, and porcine mesenchymal stem cells. Alternatively, two microfluidic devices were designed to pattern …
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Effect of broccoli bioactives on prostate cancer cell invasion in vitro
… vitro model system. DU145 cells were plated into Matrigel invasion chambers placed in 24-well plates with complete growth medium as a chemo-attractant. In the chambers, cells were treated with sulforaphane (SF), indole-3-carbinol (I3C), hydrolyzed neoglucobrassicin (hNGB), and quercetin (Q) …
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Biomimetic and vascularized 3-d liver cancer model
… chitosan mat tube are embedded together on Matrigel. Using the hanging drop method, spheroids are formed and encapsulated in collagen before exposure to the anti-cancer drug doxorubicin. Cell viability, bile canaliculi, and cytochrome p450 activity are measured afterwards, showing greater …
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Rapid formation of cell-mediated microvascular networks in collagen, fibrin, and puramatrix provisional matrices
… collagen I, fibrin, and PuraMatrix, as well as Matrigel. Vessel networks, lined by ECFCs and overlaid by alpha αSMA+ perivascular cells, formed at 50 vessels/mm^2 in collagen and fibrin ECMs, 100 vessels/mm^2 in Matrigel, and 150 vessels/mm^2 in PuraMatrix. When their viscoelastic properties …
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Developing a clinically applicable hydrogel for the treatment of liver disease
… engineering and cell delivery. For example, Matrigel is the most commonly used matrix in hepatocyte differentiation from pluripotent stem cells and liver organoid culture, even though Matrigel is prohibited from clinical use. The work for this Doctor of Philosophy was aimed to address the …
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Tierexperimentelle Untersuchungen zur Adipogenese in Gel-Trägern als Weichgewebsersatz
… preadipocytes. Three different biomaterials (matrigel, collagen g , HYADD-3) were examined regarding their potency to induce adipose tissue. All three gels were implanted in a standardised chamber made out of acetal polymer into immune deficient naked rats. In order to assess the angiogenetic …
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CHARACTERIZING THE MICRO-SCALE MECHANICAL PROPERTIES OF HYDROGELS AND CANCER CELLS
… fibrin, hyaluronic acid, kerateine, laminin, Matrigel, and polyethylene glycol diacrylate (PEGDA), were tested with atomic force microscopy (AFM). We also determined the Young’s modulus (E) of hydrogels with different concentrations. E tended to increase with increased concentration.
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Candidate tumour markers and potential therapeutic targets in colorectal cancer.
… with the DPEP-1 construct migrated through a Matrigel™ invasion assay in greater numbers than untreated cells (P=O.007). RNA interference of DPEP-1 found a significant difference in migration capacity between the mock transfected (MT) cells when compared to DPEP-1 siRNA treated cells …
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Beta 3 integrins : negative regulators of angiogenesis
… occurs in in vivo angiogenesis assayed using a Matrigel plug insert. Similarly to in vitro assays, tumstatin failed to inhibit angiogenesis in beta 3 integrin-deficient animals. These results suggest that avf33 integrin is necessary but not sufficient for the activity of tumstatin. Further …
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Three-dimensional Hyaluronan-based Hydrogels Designed for Breast Cancer Spheroid Formation
… were less sensitive compared to cells grown on Matrigel™, a complex protein extract without HA. Breast CS expressed multidrug resistance protein 1, a drug efflux transporter on bifunctional HA hydrogels, and showed decreased doxorubicin drug penetration. Secondly, HA-oxime hydrogels were …
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Regulatory Immune Responses and Repair Mechanisms in Atherosclerosis
… antigens. In paper I, we developed a matrigel-based method to characterize T helper 2 immune responses against human apolipoprotein B100 (ApoB100). We report that matrigel loaded with the antigen of interest can be used to measure antigen-specific immune cell accumulation and cytokine …
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Delivery of hiPSC Derived Cardiomyocytes via an Injectable Nanofibrous Gelatin Polyurethane Composite Scaffold for Heart Tissue Repair
… and injected into rat LV myocardium within a Matrigel-free solution. After 14 days, retained hiPSC-CMs were identified in the group receiving micro-sized composite scaffolds pre-seeded with hiPSC-CMs, but not in the control groups receiving Matrigel-free solutions containing either free cells …
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Investigation into the role of HMGb1 in relation to myofibroblasts and cancer cells exposed to various conditions
… CCD18 myofibroblasts migration and invasion in matrigel membrane invasion assay setup. The data suggested that these proteases are not upregulated in this set up and thought not to play any major role in the migration and invasion of myofibroblast cells. There remains a possibility for other …
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Empleo de andamios tridimensionales celularizados estimulados mecánica y eléctricamente para la creación de tejido musculo-esquelético
… diferentes a testar; colágeno, colágeno y Matrigel; colágeno, Matrigel, fibrina y albúmina de suero bovino y el resto biotintas de colágeno y alginato a diferentes concentraciones. Se han usado 3 andamios de electrospinning de PCL y gelatina con diferente grado de alineamiento entre sus …
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In vitro permeability studies of antitumor compounds, thioxanthones across two model barriers, and their availability in vivo
… of Engelbreth-Holm Swarm (EHS) mouse sarcoma (Matrigel) and human colorectal adenocarcinoma (Caco-2) multilayers were developed. The permeability characteristics of SR271425 (<em>N</em>-[1-{ [2-(diethylamino) ethyl] amino} -7 -methoxy-9-oxo-9H -thioxanthen-4-yl] methylformamide) a novel …
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Auswirkungen der maternalen HIV-1-Infektion auf die Genexpression endothelialer Vorläuferzellen und differenzierter Endothelzellen nicht-infizierter Kinder
… zur Ausbildung kapillarähnlicher Strukturen in Matrigel und der Expression charakteristischer Endothelzellmarker beobachtet werden. Allerdings zeigte der molekularbiologische Vergleich der Genexpression, dass in Endothelzellen von Kindern HIV-1-infizierter Mütter die Expression von …
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Human trophoblast survival and invasion in the developing placenta: autocrine regulation by hbegf
… invasive CTBs and is also induced by culture on Matrigel, but not on growth factor-reduced Matrigel. TUNEL assays indicate that HBEGF signals through the MAPK14 pathway to inhibit apoptosis in CTBs induced by 2% O<sub>2</sub> or reoxygenation injury. Previous studies have identified that HBEGF is …
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The Src- and Abl- Dependent Regulation of Net1A In Breast Cancer
… cells significantly increases cell motility and Matrigel invasion. Moreover, Net1A is required for Abl1- stimulated cell motility, which is rescued by expression of Net1A Y373D, but not Net1A Y373F. This work demonstrates a novel mechanism controlling Net1A subcellular localization to regulate …
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Optimization of cell adhesion environments for a liver cell bioreactor
… (ECM) signaling through the use of soluble Matrigel or adhesion protein-coated microspheres did not improve hepatocyte viability or function as assessed by liver-specific gene expression. These results suggest the importance of cell-cell rather than cell-surface interactions in maintaining …
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