Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Mass cytometry"”.
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Organotellurium Reagents for Multiparametric Interrogation of Dynamic Biology
… members of a population. The recent emergence of mass cytometry (MC) has offered a new solution to this problem: the ability to simultaneously measure a large number of parameters on a single cell. Unlike traditional fluorescence-based flow cytometry, which suffers from spectral overlap of …
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Synthesis, Characterization, and Application of Metal-chelating Polymers for Mass Cytometric Bioassays
… and application of metal-chelating polymers for mass-cytometric bioassays. Mass cytometry is a cell characterization technique in which cells are injected individually into an ICP-MS detector. Signal is provided by staining cell-surface or intracellular antigens with metal-labeled antibodies …
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NK cell determinants of immunity to mycobacterium tuberculosis in humans
… who were followed up over two years. Using mass cytometry, results showed that cytokine responses by NK cells in the peripheral blood were lower at distal timepoints but increased significantly with time to diagnosis in those who progressed to TB, compared to those who controlled Mtb …
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A Single Cell Approach to TCR Signal Strength
… unclear. Utilising single-cell methods including mass cytometry and single-cell RNA-sequencing, this study demonstrates how naïve and restimulated cytotoxic T cells coordinate their responses against peptides of differing stimulation strengths.
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Investigation of the synovial tissue-homing mechanisms of circulating mononuclear phagocytes in treatment-naïve psoriatic arthritis.
… MPs subsets. I used this to create a suspension mass cytometry panel to further explore 25-different tissue homing markers on blood MPs from healthy, PsA, and cutaneous psoriasis without arthritis (PsC) donors. I discovered a subset of cMos enriched for dendritic cell specific transmembrane …
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Innate Lymphoid Cell Diversity in Pregnancy and Ovarian Cancer
… tissue. To address this, I have developed a mass cytometry panel and stained resting and stimulated ILCs from peripheral blood, decidua and ascites. Within the decidua, a number of ILC subsets are identifiable including three main subsets of decidual NK cells (dNK), dNK1-3. The most abundant …
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Fibroblast Heterogeneity In Pancreatic Cancer Immunity
… <p>We employed single cell RNA sequencing, mass cytometry, and multiplex immunohistochemistry to assess genetically engineered mouse models (GEMMs) in which we could deplete aSMA<sup>+</sup> and FAP<sup>+</sup> CAFs. We found the aSMA<sup>+</sup> and FAP<sup>+</sup> CAFs were distinct …
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Ketogenic Diet is Protective During Endotoxin-Induced Lung Injury
… a proteomic-based array, ion chromatography–mass spectrometry (IC-MS), and multiplex assays in bronchial alveolar lavage fluid, as well as immune mass cytometry studies on lung tissue, we discovered that the ketogenic diet attenuates immune cell chemotaxis. In addition, treating mice with …
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Engineering B Cells For Use In Immunotherapy and Cancer
… that displayed suppressive capabilities and used mass cytometry to characterize their phenotype. However, the immunoregulatory function of B cells was transient. Interrogation of the expanded B cells at the single cell level revealed multiple markers that could potentially be modified to maintain …
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Bayesian causal inference of cell signal transduction from proteomics experiments
… analysis of single-cell "snapshot" data such as mass cytometry, where each cell is a multivariate cell-level replicate of signal transduction at a single time point. We also address the role of large-scale bulk experiments such as mass-spectrometry-based proteomics, and small-scale time-course …
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Chemokine Ligand-3 (CCL3) as a Novel Mediator of Inflammatory Bowel Disease Activity
… immunostained for CCL3, CCR1, and CCR5. Imaging mass cytometry (IMC) was performed to identify CCL3 cell type expression and CCL3-expressing cell spatial relationships. Immunohistochemistry found that there was no correlation between the severity of inflammation and percent of CCL3 positive cells …
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Genomic aberrations as determinants of immune infiltrates in high grade serous ovarian carcinoma
… analysed using gene expression data and imaging mass cytometry will also be discussed.
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Microglial succinate receptor 1 (SUCNR1) sustains chronic CNS inflammation
… numbers of microglia seen via ex vivo suspension mass cytometry (CyTOF) immunophenotyping. Mechanistically, I found that SUCNR1 has opposite effects on macrophages, where it attenuates their pro-inflammatory activation, compared to microglia, where it promotes the response to both pro-inflammatory …
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The immunopathogenic mechanism of severe cutaneous adverse reactions to first line anti-tuberculosis drugs
… an integrated single-cell approach involving: i) mass cytometry by time of flight (n=8), and ii) single cell RNA sequencing (scRNA-seq) (n=3) to characterise immune cells activated by drug. Results Forty-one RegiSCAR validated SCAR cases that reacted to one or more FLTD on rechallenge were …
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Imaging Post-Infarct Myocardial Inflammation with ⁶⁸Ga-DOTATATE PET/MRI and insights into COVID-19 associated myocardial injury
… of circulating immune cell subsets using mass cytometry by time-of-flight (CyTOF) and for serum proteomic analysis. <sup>68</sup>Ga-DOTATATE maximum Standardised Uptake Values (SUV<sub>max</sub>) were derived for myocardial segments with late gadolinium enhancement (LGE; “infarct”) as well …
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The Role of Ifitm3 In The Immune Response of Brca-Deficient High Grade Serous Ovarian Carcinoma
… multiplex immunofluorescence (IF) and imaging mass cytometry (IMC) on our cohort, and HLA mRNA expression was evaluated in HGSOC cell lines. IFITM3 expression was manipulated in HGSOC cells using siRNAs to determine the effect of IFITM3 on the expression of immune-related genes and survival in …
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Mapping the spatial organisation of B cells and immune signalling in TNBC to characterise response to immunotherapy
… of protein and RNA in situ using imaging mass cytometry. We applied this assay to a randomised immunotherapy clinical trial, NeoTRIP, in which patients with TNBC were treated with atezolizumab. Samples were collected from 268 patients at three timepoints: baseline, on-treatment, and …
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Lymphocyte subset variability between human lymphoid tissues
… and spleen from human cadaver were analysed by mass cytometry (cytometry by time of flight [CyTOF]) for deep phenotypic profiling and tissue-specific subset variation. Populations of B and T cells that had significant abundance differences between the three tissues were identified in an …
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Phenotypic and Functional Characterisation of Hofbauer Cells Across Gestation
… and sessile by full term. A high-dimensional mass cytometry (CyTOF) panel was developed and used to show that within the first trimester placenta HBC were a homogeneous population, lacking expression of the HLA class II marker HLA-DR. This contrasted with full-term placenta where HBC displayed …
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