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Showing 1 to 7 of 7 for “"Masp1"”.
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Sequence-structure correlations in the MaSp1 protein of N. clavipes dragline silk
… structures of wildtype as well as modified MaSp1 protein from the N. clavipes spider dragline silk sequences are reported, obtained using an in silico approach based on replica exchange molecular dynamics (REMD) and explicit water molecular dynamics. In particular, the atomistic simulations …
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Characterization of a family of cysteine rich proteins and development of a MaSp1 derived miniature fibroin
… along with several internal block repeats of MaSp1. We also characterize a family of small cysteine-rich proteins (CRPs) and demonstrate that these factors are present within the spinning dope of the major ampullate gland using MS analysis. Biochemical studies and characterization of one of …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
… this study, we developed several codon-optimized MaSp1 minifibroin constructs for recombinant protein expression in bacteria. These recombinant MaSp1 minifibroin constructs were engineered to contain the N-terminal domain (NTD), different copies of internal block repeats (ranging from 2 to 64 …
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Funktion und Einfluss der nicht-repetitiven, terminalen Domänen auf Speicherung und Assemblierung von Spinnenseidenproteinen
… aus zwei Proteinklassen aufgebaut, die als MaSp1 und MaSp2(Major Ampullate Spidroin) bezeichnet werden. Die mehrere hundert Kilodalton großen Spidroine bestehen im Wesentlichen aus einer repetitiven, strukturell ungeordneten Kerndomäne, die von relativ kleinen, ca. 100-140 Aminosäuren langen …
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Evidence that aciniform silk and minor ampullate silk are major constituents of wrapping silk from the black widow
… contains the fibroins AcSp1-like, MiSp1-like, MaSp1 and MaSp2. These are the first studies to reveal that minor, major and acinifonn silks are constituents of wrapping material.</p>
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Characterizing small molecular weight proteins from Latrodectus hesperus dragline and tubuliform silks
… CRP1 protein and its ability to bind MaSp1 components using pull down assays to determine potential interactions. No substantial biochemical evidence was produced to demonstrate protein-protein interactions between the two. Additionally, we show that using RT-PCR analysis from mRNAs …